Updated on 2024/02/15

写真a

 
HAYAKAWA Takeshi
 
Organization
Faculty of Science and Engineering Associate Professor
Other responsible organization
Precision Engineering Course of Graduate School of Science and Engineering, Master's Program
Precision Engineering Course of Graduate School of Science and Engineering, Doctoral Program
Contact information
The inquiry by e-mail is 《here
External link

Degree

  • 博士(工学) ( 名古屋大学 )

  • 修士(理学) ( 東京理科大学 )

Education

  • 2015.3
     

    Nagoya University   Graduate School, Division of Engineering   doctor course   completed

  • 2009.3
     

    Tokyo University of Science   Graduate School, Division of Natural Science   master course   completed

  • 2007.3
     

    Tokyo University of Science   Faculty of Science   graduated

  • 2003.3
     

    埼玉県立春日部高等学校   graduated

Research History

  • 2020.4 -  

    中央大学理工学部准教授

  • 2017.4 - 2020.3

    中央大学理工学部助教

  • 2016.4 - 2017.3

    名古屋大学大学院 未来社会創造機構 特任助教

  • 2015.4 - 2016.3

    名古屋大学大学院 工学研究科 マイクロ・ナノシステム工学専攻 特任助教

  • 2009.4 - 2012.3

    テルモ株式会社研究開発本部商品開発グループ 兼 MEセンターME開発グループ

Professional Memberships

  • Japan Society of Precision Engineering

  • 計測自動制御学会

  • 日本機会学会

  • 化学とマイクロ・ナノシステム学会

  • 日本ロボット学会

  • The Institute of Electrical and Electronics Engineers, Inc. (IEEE)|rn|Robotics and Automation Society

▼display all

Research Interests

  • Micro/ Nano- Robotics

Research Areas

  • Nanotechnology/Materials / Nano/micro-systems  / Nano/Microsystems

  • Informatics / Robotics and intelligent system  / 知能機械学・機械システム

  • Life Science / Biomedical engineering  / Biomedical engineering/Biomaterial science and engineering

  • Informatics / Mechanics and mechatronics  / 知能機械学・機械システム

  • Life Science / Biomaterials  / Biomedical engineering/Biomaterial science and engineering

Papers

  • Rotation of Biological Cells: Fundamentals and Applications

    Tao Tang, Yoichiroh Hosokawa, Takeshi Hayakawa, Yo Tanaka, Weihua Li, Ming Li, Yaxiaer Yalikun

    ENGINEERING   10   110 - 126   2022.3

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    Cell rotation is one of the most important techniques for cell manipulation in modern bioscience, as it not only permits cell observation from any arbitrary angle, but also simplifies the procedures for analyzing the mechanical properties of cells, characterizing cell physiology, and performing microsurgery. Numerous approaches have been reported for rotating cells in a wide range of academic and industrial applications. Among them, the most popular are micro-robot-based direct contact manipulation and field-based non-contact methods (e.g., optical, magnetic, electric, acoustic, and hydrodynamic methods). This review first summarizes the fundamental mechanisms, merits, and demerits of these six main groups of approaches, and then discusses their differences and limitations in detail. We aim to bridge the gap between each method and illustrate the development progress, current advances, and prospects in the field of cell rotation. CO 2021 THE AUTHORS. Published by Elsevier LTD on behalf of Chinese Academy of Engineering and Higher Education Press Limited Company. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

    DOI: 10.1016/j.eng.2020.07.031

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  • Evaluation of the Response Characteristics of On-Chip Gel Actuators for Various Single Cell Manipulations Reviewed

    Hiroki Wada, Yuha Koike, Yoshiyuki Yokoyama, Takeshi Hayakawa

    IEEE ROBOTICS AND AUTOMATION LETTERS   5 ( 4 )   5205 - 5212   2020.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC  

    On-chip gel actuators are potential candidates for single cell manipulation because they can realize low-invasive manipulation of various cells. We propose an on-chip gel actuator driven by light irradiation. By patterning the gel actuator with light absorber, we can control the temperature of the actuator and drive it. The proposed drive method can realize highly localized temperature control of the gel actuator and can be applied to mass integration of on-chip gel actuators. In this study, we evaluate the heat conduction of the actuator during driving and its response characteristics as a function of various design parameters. We theoretically and experimentally evaluate the response characteristics and confirm that the response characteristics can be changed by altering the size of the light absorber. Furthermore, we show some examples of cell manipulation including trapping, transport, and sorting with various sizes of light absorber. Finally, we show proof of concept for the application of the proposed drive method for massive integration of on-chip gel actuators.

    DOI: 10.1109/LRA.2020.3006792

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  • Raman image-activated cell sorting Reviewed

    Nao Nitta, Takanori Iino, Akihiro Isozaki, Mai Yamagishi, Yasutaka Kitahama, Shinya Sakuma, Yuta Suzuki, Hiroshi Tezuka, Minoru Oikawa, Fumihito Arai, Takuya Asai, Dinghuan Deng, Hideya Fukuzawa, Misa Hase, Tomohisa Hasunuma, Takeshi Hayakawa, Kei Hiraki, Kotaro Hiramatsu, Yu Hoshino, Mary Inaba, Yuki Inoue, Takuro Ito, Masataka Kajikawa, Hiroshi Karakawa, Yusuke Kasai, Yuichi Kato, Hirofumi Kobayashi, Cheng Lei, Satoshi Matsusaka, Hideharu Mikami, Atsuhiro Nakagawa, Keiji Numata, Tadataka Ota, Takeichiro Sekiya, Kiyotaka Shiba, Yoshitaka Shirasaki, Nobutake Suzuki, Shunji Tanaka, Shunnosuke Ueno, Hiroshi Watarai, Takashi Yamano, Masayuki Yazawa, Yusuke Yonamine, Dino Di Carlo, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki, Keisuke Goda

    NATURE COMMUNICATIONS   11 ( 1 )   2020.7

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    <title>Abstract</title>The advent of image-activated cell sorting and imaging-based cell picking has advanced our knowledge and exploitation of biological systems in the last decade. Unfortunately, they generally rely on fluorescent labeling for cellular phenotyping, an indirect measure of the molecular landscape in the cell, which has critical limitations. Here we demonstrate Raman image-activated cell sorting by directly probing chemically specific intracellular molecular vibrations via ultrafast multicolor stimulated Raman scattering (SRS) microscopy for cellular phenotyping. Specifically, the technology enables real-time SRS-image-based sorting of single live cells with a throughput of up to ~100 events per second without the need for fluorescent labeling. To show the broad utility of the technology, we show its applicability to diverse cell types and sizes. The technology is highly versatile and holds promise for numerous applications that are previously difficult or undesirable with fluorescence-based technologies.

    DOI: 10.1038/s41467-020-17285-3

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    Other Link: http://www.nature.com/articles/s41467-020-17285-3

  • Light-Driven Hydrogel Microactuators for On-Chip Cell Manipulations Reviewed

    Yuha Koike, Yoshiyuki Yokoyama, Takeshi Hayakawa

    Frontiers in Mechanical Engineering   6   2   2020.1

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    DOI: 10.3389/fmech.2020.00002

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  • Intelligent Image-Activated Cell Sorting Reviewed International journal

    Nao Nitta, Takeaki Sugimura, Akihiro Isozaki, Hideharu Mikami, Kei Hiraki, Shinya Sakuma, Takanori Iino, Fumihito Arai, Taichiro Endo, Yasuhiro Fujiwaki, Hideya Fukuzawa, Misa Hase, Takeshi Hayakawa, Kotaro Hiramatsu, Yu Hoshino, Mary Inaba, Takuro Ito, Hiroshi Karakawa, Yusuke Kasai, Kenichi Koizumi, SangWook Lee, Cheng Lei, Ming Li, Takanori Maeno, Satoshi Matsusaka, Daichi Murakami, Atsuhiro Nakagawa, Yusuke Oguchi, Minoru Oikawa, Tadataka Ota, Kiyotaka Shiba, Hirofumi Shintaku, Yoshitaka Shirasaki, Kanako Suga, Yuta Suzuki, Nobutake Suzuki, Yo Tanaka, Hiroshi Tezuka, Chihana Toyokawa, Yaxiaer Yalikun, Makoto Yamada, Mai Yamagishi, Takashi Yamano, Atsushi Yasumoto, Yutaka Yatomi, Masayuki Yazawa, Dino Di Carlo, Yoichiroh Hosokawa, Sotaro Uemura, Yasuyuki Ozeki, Keisuke Goda

    CELL   175 ( 1 )   266 - +   2018.9

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    A fundamental challenge of biology is to understand the vast heterogeneity of cells, particularly how cellular composition, structure, and morphology are linked to cellular physiology. Unfortunately, conventional technologies are limited in uncovering these relations. We present a machine-intelligence technology based on a radically different architecture that realizes real-time image-based intelligent cell sorting at an unprecedented rate. This technology, which we refer to as intelligent image-activated cell sorting, integrates high-throughput cell microscopy, focusing, and sorting on a hybrid software-hardware data-management infrastructure, enabling real-time automated operation for data acquisition, data processing, decision-making, and actuation. We use it to demonstrate real-time sorting of microalgal and blood cells based on intracellular protein localization and cell-cell interaction from large heterogeneous populations for studying photosynthesis and atherothrombosis, respectively. The technology is highly versatile and expected to enable machine-based scientific discovery in biological, pharmaceutical, and medical sciences.

    DOI: 10.1016/j.cell.2018.08.028

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  • A surgical simulator for peeling the inner limiting membrane during wet conditions Reviewed International journal

    Seiji Omata, Yusei Someya, Shynya Adachi, Taisuke Masuda, Takeshi Hayakawa, Kanako Harada, Mamoru Mitsuishi, Kiyohito Totsuka, Fumiyuki Araki, Muneyuki Takao, Makoto Aihara, Fumihito Arai

    PLOS ONE   13 ( 5 )   e0196131   2018.5

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    The present study was performed to establish a novel ocular surgery simulator for training in peeling of the inner limited membrane (ILM). This simulator included a next-generation artificial ILM with mechanical properties similar to the natural ILM that could be peeled underwater in the same manner as in actual surgery. An artificial eye consisting of a fundus and eyeball parts was fabricated. The artificial eye was installed in the eye surgery simulator. The fundus part was mounted in the eyeball, which consisted of an artificial sclera, retina, and ILM. To measure the thickness of the fabricated ILM on the artificial retina, we calculated the distance of the step height as the thickness of the artificial ILM. Two experienced ophthalmologists then assessed the fabricated ILM by sensory evaluation. The minimum thickness of the artificial ILM was 1.9 ± 0.3 μm (n = 3). We were able to perform the peeling task with the ILM in water. Based on the sensory evaluation, an ILM with a minimum thickness and 1000 degrees of polymerization was suitable for training. We installed the eye model on an ocular surgery simulator, which allowed for the performance of a sequence of operations similar to ILM peeling. In conclusion, we developed a novel ocular surgery simulator for ILM peeling. The artificial ILM was peeled underwater in the same manner as in an actual operation.

    DOI: 10.1371/journal.pone.0196131

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  • On-chip cell sorting by high-speed local-flow control using dual membrane pumps Reviewed

    Shinya Sakuma, Yusuke Kasai, Takeshi Hayakawa, Fumihito Arai

    LAB ON A CHIP   17 ( 16 )   2760 - 2767   2017.8

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    Although researchers have proposed various methods of on-chip cell sorting, high-throughput sorting of large cells remains hampered by the difficulty of controlling high-speed flow over a wide sorting area. To overcome this problem, we proposed high-speed local-flow control using dual membrane pumps driven by piezoelectric actuators placed on the outside of a microfluidic chip in this paper. We evaluated the controllability of shifting the flow profile by the local-flow. The results indicated that we could sort large cells up to approximately 150 mu m in size with an equivalent throughput of 31 kHz. Because our method can control the flow profiles, it is applicable not only to large cells but also to small cells. The cell-sorting efficacy of the proposed method was experimentally evaluated on Euglena gracilis NIES-48 (E. gracilis) cells as large target cells and GCIY-EGFP (GCIY) cells derived from a gastric cancer cell line as small target cells. In E. gracilis cells sorting, the throughput is 23 kHz with a 92.8% success rate, 95.8% purity, and 90.8% cell viability. In GCIY sorting, the throughput is 11 kHz with a 97.8% success rate, 98.9% purity, and 90.7% cell viability. These results confirm that the proposed method sorts differently sized cells with high throughput and hence, overcomes the throughput-size trade-off that exists in conventional on-chip cell sorters.

    DOI: 10.1039/c7lc00536a

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  • Optically driven micro- and nanorobots

    Hayakawa Takeshi, Maruyama Hisataka, Arai Fumihito

    LIGHT ROBOTICS STRUCTURE-MEDIATED NANOBIOPHOTONICS   193-236   2017

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    DOI: 10.1016/B978-0-7020-7096-9.00007-0

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  • On-chip 3D rotation of oocyte based on a vibration-induced local whirling flow Reviewed

    Takeshi Hayakawa, Shinya Sakuma, Fumihito Arai

    MICROSYSTEMS & NANOENGINEERING   1 ( 1 )   1 - 9   2015

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    We propose a novel on-chip 3D cell rotation method based on a vibration-induced flow. When circular vibration is applied to a microchip with micropillar patterns, a highly localized whirling flow is induced around the micropillars. The direction and velocity of this flow can be controlled by changing the direction and amplitude of the applied vibration. Furthermore, this flow can be induced on an open chip structure. In this study, we adopted a microchip with three micropillars arranged in a triangular configuration and an xyz piezoelectric actuator to apply the circular vibration. At the centre of the micropillars, the interference of the vibration- induced flows originating from the individual micropillars induces rotational flow. Consequently, a biological cell placed at this centre rotates under the influence of the flow. Under three-plane circular vibrations in the xy, xz or yz plane, the cell can rotate in both the focal and vertical planes of the microscope. Applying this 3D cell rotation method, we measured the rotational speeds of mouse oocytes in the focal and vertical planes as 63.7 +/- 4.0 degrees s(-1) and 3.5 +/- 2.1 degrees s(-1), respectively. Furthermore, we demonstrated the transportation and rotation of the mouse oocytes and re-positioned their nuclei into a position observable by microscope.

    DOI: 10.1038/micronano.2015.1

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  • Pneumatic Microballoons for Active Control of the Vibration-Induced Flow

    Taku Sato, Kanji Kaneko, Takeshi Hayakawa, Hiroaki Suzuki

    Micromachines   14 ( 11 )   2010 - 2010   2023.10

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    Vibration-induced flow (VIF), in which a mean flow is induced around a microstructure by applying periodic vibrations, is increasingly used as an active flow-control technique at the microscale. In this study, we have developed a microdevice that actively controls the VIF patterns using elastic membrane protrusions (microballoons) actuated by pneumatic pressure. This device enables on-demand spatial and temporal fluid manipulation using a single device that cannot be achieved using a conventional fixed-structure arrangement. We successfully demonstrated that the device achieved displacements of up to 38 µm using the device within a pressure range of 0 to 30 kPa, indicating the suitability of the device for microfluidic applications. Using this active microballoon array, we demonstrated that the device can actively manipulate the flow field and induce swirling flows. Furthermore, we achieved selective actuation of the microballoon using this system. By applying air pressure from a multi-input channel system through a connection tube, the microballoons corresponding to each air channel can be selectively actuated. This enabled precise control of the flow field and periodic switching of the flow patterns using a single chip. In summary, the proposed microdevice provides active control of VIF patterns and has potential applications in advanced microfluidics, such as fluid mixing and particle manipulation.

    DOI: 10.3390/mi14112010

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  • Investigation of the vibration-induced local flow around a micro-pillar under various vibration conditions

    Kanji KANEKO, Zhitai HUANG, Taku SATO, Naoto UJIKAWA, Takeshi HAYAKAWA, Yosuke HASEGAWA, Hiroaki SUZUKI

    Mechanical Engineering Journal   10 ( 1 )   22 - 00223   2023

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    DOI: 10.1299/mej.22-00223

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  • Fabrication of a Spherical-Shape Dielectric Elastomer Actuator Made of an Organogel

    Yuta Tezuka, Asahi Tsujino, Takeshi Hayakawa

    2023 IEEE/SICE INTERNATIONAL SYMPOSIUM ON SYSTEM INTEGRATION, SII   2023

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    In this study, we fabricated three-dimensional (3D) DEA made of an organogel and drove it out-of-plane direction using an experimental system that applied pre-stretch with water pressure. Organogels have a lower Young's modulus and higher relative permittivity than elastomer materials used in conventional DEA such as PDMS. Therefore, fabrication of DEAs using organogel is expected to increase the displacement of DEAs. Thus, we fabricated a dielectric gel that is a kind of organogel with high dielectric constant and excellent elasticity. We evaluated static characteristics and frequency response of water-pre-stretched DEA made of dielectric gel. We also fabricated three-dimensional dielectric gel for a 3D shaped DEA. From a driving experiment, it was confirmed that this 3D DEA can generate 10 times larger displacement than that of PDMS.

    DOI: 10.1109/SII55687.2023.10039186

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  • Analysis of Dynamic Characteristics of a Balloon-Type Dielectric Elastomer Actuator Pre-Stretched by Water Pressure

    Asahi Tsujino, Takeshi Hayakawa

    2023 IEEE/SICE INTERNATIONAL SYMPOSIUM ON SYSTEM INTEGRATION, SII   2023

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    In this paper, we analyzed dynamic characteristics of a balloon-type dielectric elastomer actuator (DEA) prestretched by water pressure. We derived an analytical solution for dynamic characteristics of this DEA based on a physical model of this experimental system. This experimental system consists of an elastomer attached on an acrylic pipe and a water reservoir connected to the acrylic pipe with a hose. Therefore, by changing parameters of these equipment, dynamic characteristics will be changed. We analyzed frequency response of the DEA when we changed these parameters and compared experimental values with theoretical values. Also, we analyzed dynamic responses when various electric voltages were applied to the DEA. The analytical solution can predict frequency responses and dynamic response. In addition, we conducted open loop control of displacement of the DEA. Deformations of DEA was able to be controlled according to desired displacement with the constructed model.

    DOI: 10.1109/SII55687.2023.10039139

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  • Microgripper Using Soft Microactuators for Manipulation of Living Cells. International journal

    Shunnosuke Kodera, Tomoki Watanabe, Yoshiyuki Yokoyama, Takeshi Hayakawa

    Micromachines   13 ( 5 )   2022.5

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    We present a microgripper actuated by a soft microactuator for manipulating a single living cell. Soft actuators have attracted attention in recent years because their compliance which can adapt to soft targets. In this study, we propose a microgripper actuated by soft thermoresponsive hydrogels. The thermoresponsive gel swells in water when the temperature is low and shrinks when the temperature is high. Therefore, the microgripper can be driven by controlling the temperature of the thermoresponsive gel. The gels are actuated by irradiating with infrared (IR) laser to localize heating. The actuation characteristics of the gripper were theoretically analyzed and we designed a gripper that gripped a ≈10 µm size cell. Additionally, we succeeded in actuating the fabricated microgripper with laser irradiation and gripping a single living cell.

    DOI: 10.3390/mi13050794

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  • Detection of nanoparticles in a minute sample using the vibration induced flow

    Kanji Kaneko, Mamiko Tsugane, Taku Sato, Takeshi Hayakawa, Yosuke Hasegawa, Hiroaki Suzuki

    2022 IEEE 17th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)   2022.4

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    DOI: 10.1109/nems54180.2022.9791090

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  • Real-time irradiation system using patterned light to actuate light-driven on-chip gel actuators Reviewed

    Yuha Koike, Shunnosuke Kodera, Yoshiyuki Yokoyama, Takeshi Hayakawa

    ROBOMECH Journal   9 ( 1 )   1 - 10   2022.2

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  • レーザ照射を用いた蠕動運動型マイクロゲルロボットの駆動 Reviewed

    小寺 駿之亮, 小池 優巴, 横山 義之, 早川 健

    日本ロボット学会誌   40 ( 1 )   83 - 86   2022.1

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    Soft robots have been attracting a lot of attentions in recent years because they have completely different characteristics from conventional robots having rigid structures. We have proposed a soft microrobot using a thermoresponsive gel as a soft actuator. This robot can be driven by being irradiated with light for controlling its temperature. In this study, we drive a robot having three gel actuators with a straight shape with peristaltic motion by scanning the robot with a laser. We evaluated actuation timing of each actuator and a displacement of the robot during the peristaltic motion.

    DOI: 10.7210/jrsj.40.83

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  • 集積型ゲルアクチュエータを用いた並列細胞操作システム Reviewed

    小池 優巴, 和田紘樹, 小寺 駿之亮, 横山 義之, 早川 健

    日本ロボット学会誌   39 ( 7 )   673 - 676   2021.9

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:日本ロボット学会  

    On-chip actuator is an essential component to realize single cell analysis in microfluidic device. Previously, we proposed a light-driven gel actuator for single cell manipulations. The advantage of this drive method is it enables local heating for integration of large number of actuators on a microfluidic chip. In this study, we present multiple cell manipulations system by using integrated light-driven gel actuators. We construct the microscope to irradiate patterned light by using digital mirror device. Therefore, we can drive multiple gel actuators selectively. Finally, we demonstrate an example of cell manipulations by using integrated gel actuators and the constructed system.

    DOI: 10.7210/jrsj.39.673

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  • オンチップゲルアクチュエータの三次元形状および駆動特性評価 Reviewed

    和田 紘樹, 小池 優巴, 横山 義之, 早川 健

    日本ロボット学会誌   39 ( 6 )   565 - 568   2021.7

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    Previously, we proposed new light irradiation-based drive method of on-chip gel actuator. In our method, on-chip gel actuators are fixed on a glass substrate with light absorbers. Therefore, deformation of the gel is constricted with fixed part and anisotropically deforms in three dimensions. In this study, we evaluate the three-dimensional shapes and drive characteristics of on-chip gel actuator. Furthermore, we evaluate changes of drive characteristics of the actuators with respect to surface area to volume ratio. From this result, we show the possibilities of control of gel deformation and drive characteristics by changing the design of the proposed gel actuator.

    DOI: 10.7210/jrsj.39.565

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  • Evaluation of the deformation shape of a balloon‑type dielectric elastomer actuator prestretched with water pressure Reviewed

    Natsumi Koike, Takeshi Hayakawa

    ROBOMECH Journal   8 ( 2 )   1 - 1-   2021.1

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  • Laser Scanning Drive of the Peristaltic Micro-Gelrobot with Soft Rigid Hybrid Structures

    Shunnosuke Kodera, Tomoki Watanabe, Yoshiyuki Yokoyama, Takeshi Hayakawa

    2021 IEEE/SICE INTERNATIONAL SYMPOSIUM ON SYSTEM INTEGRATION (SII)   83 - 86   2021

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    We present a micro-gelrobot driven by irradiation of a laser. The proposed micro-gelrobot mimics living organisms and moves by peristaltic motion. In this paper, we show the micro-gelrobot has multiple soft structures and rigid structures. The soft structures work as actuators like muscles and rigid strictures work as supporting bodies like skeletons. As rigid structures, SU-8 which is hard photoresist is used. As soft actuators, bioresist which is a photo-patternable temperature-responsive gel is used. Bioresist swells by absorbing water at lower temperature than 32 degrees C and shrinks by releasing water at higher temperature than 32 degrees C. Thus, we use this changing volume as a displacement of an actuator. In addition, we mixed bioresist with graphene as light absorber to actuate this gel actuator by near infrared laser irradiation. The proposed micro-gelrobot is fabricated by using photolithography which is a common MEMS fabrication process. Additionally, fabrication process of the proposed micro-gelrobot include release process of the robot from the glass substrate. For release of micro-gelrobot, we use sacrificial layer made of dextran which is a kind of polysaccharide. We use digital mirror device (DMD) to irradiate patterned light to actuate multiple gel actuators. We succeeded in driving the proposed micro-gelrobot using laser scanning. In addition, we evaluate a displacement of the peristaltic motion. The proposed micro-gelrobot can move forward by performing a peristaltic motion.

    DOI: 10.1109/IEEECONF49454.2021.9382636

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  • MASSIVE INTEGRATION OF LIGHT DRIVING GEL ACTUATOR FOR SINGLE CELL MANIPULATION

    Yuha Koike, Hiroki Wada, Yoshiyuki Yokoyama, Takeshi Hayakawa

    2020 33RD IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2020)   1094 - 1097   2020

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    We propose drive method of on-chip gel actuator using light irradiation for massive integration of gel actuators. The gel actuator is made of temperature responsive gel and patterned on a chip with light absorbing material. Thus, it can be driven by irradiation of light to control its temperature. By using this method, we can selectively drive an actuator among massively integrated actuators by irradiating patterned light and it can be applied to high-throughput cell manipulations. In this study, we demonstrated an example of cell manipulation by using this method. We made a flow channel for cell transportation by irradiating light. We succeeded in making straight channel by irradiating actuators with sheet laser. In this channel, we observed that motile cells moved and we succeeded in trapping the motile cells by turning off the laser and swelling the actuators.

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  • Author Correction: A practical guide to intelligent image-activated cell sorting. Reviewed International journal

    Akihiro Isozaki, Hideharu Mikami, Kotaro Hiramatsu, Shinya Sakuma, Yusuke Kasai, Takanori Iino, Takashi Yamano, Atsushi Yasumoto, Yusuke Oguchi, Nobutake Suzuki, Yoshitaka Shirasaki, Taichiro Endo, Takuro Ito, Kei Hiraki, Makoto Yamada, Satoshi Matsusaka, Takeshi Hayakawa, Hideya Fukuzawa, Yutaka Yatomi, Fumihito Arai, Dino Di Carlo, Atsuhiro Nakagawa, Yu Hoshino, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki, Nao Nitta, Keisuke Goda

    Nature protocols   14 ( 11 )   3273 - 3273   2019.11

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    An amendment to this paper has been published and can be accessed via a link at the top of the paper.

    DOI: 10.1038/s41596-019-0252-5

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  • Structurally isolated photoactuation of graphene-mixed temperature-responsive hydrogels in soft-rigid series structure Reviewed

    Tomoki Watanabe, Yoshiyuki Yokoyama, Takeshi Hayakawa

    ROBOMECH JOURNAL   6 ( 1 )   11   2019.9

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGERNATURE  

    <jats:title>Abstract</jats:title>
    <jats:p>This paper presents fabrication and actuation methods for a soft microrobot with a hybrid structure composed of soft microactuators and a rigid supporting body. This hybrid structure enables actuation of the microrobot with independent driving of multiple actuators to provide complex movement like that of living microorganisms. We use the temperature-responsive hydrogel poly(<jats:italic>N</jats:italic>-isopropylacrylamide) (PNIPAAm) as a soft microactuator. PNIPAAm swells with water at low temperature but shrinks at high temperature. This volume change thus allows PNIPAAm to be used as an actuator by controlling its temperature. We successfully fabricated the microrobot with its soft-rigid hybrid structure composed of PNIPAAm and rigid photoresist using a multilayered microfabrication process. In addition, we used a sacrificial layer process to release the fabricated microrobot from the substrate to allow it to move freely. To actuate the microrobot, we mixed PNIPAAm with graphene, which has a high photothermal conversion efficiency. The temperature of the soft actuator when mixed with graphene can be increased by irradiating it with light. Therefore, actuation of the microrobot is achieved by sequentially irradiating the microactuators with focused light. We present the fabrication, release and partial actuation of the microrobot to demonstrate the feasibility of the proposed microrobot with the soft-rigid hybrid structure in this paper.</jats:p>

    DOI: 10.1186/s40648-019-0140-3

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  • High-speed microparticle isolation unlimited by Poisson statistics

    Iino Takanori, Okano Kazunori, Lee Sang Wook, Yamakawa Takeshi, Hagihara Hiroki, Hong Zhen-Yi, Maeno Takanori, Kasai Yusuke, Sakuma Shinya, Hayakawa Takeshi, Arai Fumihito, Ozeki Yasuyuki, Goda Keisuke, Hosokawa Yoichiroh

    LAB ON A CHIP   19 ( 16 )   2669-2677 - 2677   2019.8

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    <p>We demonstrate an on-chip microparticle sorter with an ultrashort switching window using femtosecond laser pulses to overcome the fundamental limitation of the sorting performance described by Poisson statistics.</p>

    DOI: 10.1039/c9lc00324j

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  • A practical guide to intelligent image-activated cell sorting. Reviewed International journal

    Akihiro Isozaki, Hideharu Mikami, Kotaro Hiramatsu, Shinya Sakuma, Yusuke Kasai, Takanori Iino, Takashi Yamano, Atsushi Yasumoto, Yusuke Oguchi, Nobutake Suzuki, Yoshitaka Shirasaki, Taichiro Endo, Takuro Ito, Kei Hiraki, Makoto Yamada, Satoshi Matsusaka, Takeshi Hayakawa, Hideya Fukuzawa, Yutaka Yatomi, Fumihito Arai, Dino Di Carlo, Atsuhiro Nakagawa, Yu Hoshino, Yoichiroh Hosokawa, Sotaro Uemura, Takeaki Sugimura, Yasuyuki Ozeki, Nao Nitta, Keisuke Goda

    Nature protocols   14 ( 8 )   2370 - 2415   2019.8

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    Intelligent image-activated cell sorting (iIACS) is a machine-intelligence technology that performs real-time intelligent image-based sorting of single cells with high throughput. iIACS extends beyond the capabilities of fluorescence-activated cell sorting (FACS) from fluorescence intensity profiles of cells to multidimensional images, thereby enabling high-content sorting of cells or cell clusters with unique spatial chemical and morphological traits. Therefore, iIACS serves as an integral part of holistic single-cell analysis by enabling direct links between population-level analysis (flow cytometry), cell-level analysis (microscopy), and gene-level analysis (sequencing). Specifically, iIACS is based on a seamless integration of high-throughput cell microscopy (e.g., multicolor fluorescence imaging, bright-field imaging), cell focusing, cell sorting, and deep learning on a hybrid software-hardware data management infrastructure, enabling real-time automated operation for data acquisition, data processing, intelligent decision making, and actuation. Here, we provide a practical guide to iIACS that describes how to design, build, characterize, and use an iIACS machine. The guide includes the consideration of several important design parameters, such as throughput, sensitivity, dynamic range, image quality, sort purity, and sort yield; the development and integration of optical, microfluidic, electrical, computational, and mechanical components; and the characterization and practical usage of the integrated system. Assuming that all components are readily available, a team of several researchers experienced in optics, electronics, digital signal processing, microfluidics, mechatronics, and flow cytometry can complete this protocol in ~3 months.

    DOI: 10.1038/s41596-019-0183-1

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  • Label-free chemical imaging flow cytometry by high-speed multicolor stimulated Raman scattering Reviewed

    Yuta Suzuki, Koya Kobayashi, Yoshifumi Wakisaka, Dinghuan Deng, Shunji Tanaka, Chun-Jung Huang, Cheng Lei, Chia-Wei Sun, Hanqin Liu, Yasuhiro Fujiwaki, Sangwook Lee, Akihiro Isozaki, Yusuke Kasai, Takeshi Hayakawa, Shinya Sakuma, Fumihito Arai, Kenichi Koizumi, Hiroshi Tezuka, Mary Inaba, Kei Hiraki, Takuro Ito, Misa Hase, Satoshi Matsusaka, Kiyotaka Shiba, Kanako Suga, Masako Nishikawa, Masahiro Jona, Yutaka Yatomi, Yaxiaer Yalikun, Yo Tanaka, Takeaki Sugimura, Nao Nitta, Keisuke Goda, Yasuyuki Ozeki

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   116 ( 32 )   15842 - 15848   2019.8

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    Combining the strength of flow cytometry with fluorescence imaging and digital image analysis, imaging flow cytometry is a powerful tool in diverse fields including cancer biology, immunology, drug discovery, microbiology, and metabolic engineering. It enables measurements and statistical analyses of chemical, structural, and morphological phenotypes of numerous living cells to provide systematic insights into biological processes. However, its utility is constrained by its requirement of fluorescent labeling for phenotyping. Here we present label-free chemical imaging flow cytometry to overcome the issue. It builds on a pulse pair-resolved wavelength-switchable Stokes laser for the fastest-to-date multicolor stimulated Raman scattering (SRS) microscopy of fast flowing cells on a 3D acoustic focusing microfluidic chip, enabling an unprecedented throughput of up to similar to 140 cells/s. To show its broad utility, we use the SRS imaging flow cytometry with the aid of deep learning to study the metabolic heterogeneity of microalgal cells and perform marker-free cancer detection in blood.

    DOI: 10.1073/pnas.1902322116

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  • High-throughput label-free molecular fingerprinting flow cytometry Reviewed

    Kotaro Hiramatsu, Takuro Ideguchi, Yusuke Yonamine, SangWook Lee, Yizhi Luo, Kazuki Hashimoto, Takuro Ito, Misa Hase, Jee-Woong Park, Yusuke Kasai, Shinya Sakuma, Takeshi Hayakawa, Fumihito Arai, Yu Hoshino, Keisuke Goda

    SCIENCE ADVANCES   5 ( 1 )   eaau0241   2019.1

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    Flow cytometry is an indispensable tool in biology for counting and analyzing single cells in large heterogeneous populations. However, it predominantly relies on fluorescent labeling to differentiate cells and, hence, comes with several fundamental drawbacks. Here, we present a high-throughput Raman flow cytometer on a microfluidic chip that chemically probes single live cells in a label-free manner. It is based on a rapid-scan Fourier-transform coherent anti-Stokes Raman scattering spectrometer as an optical interrogator, enabling us to obtain the broadband molecular vibrational spectrum of every single cell in the fingerprint region (400 to 1600 cm(-1)) with a record--high throughput of similar to 2000 events/s. As a practical application of the method not feasible with conventional flow cytometry, we demonstrate high-throughput label-free single-cell analysis of the astaxanthin productivity and photosynthetic dynamics of Haematococcus lacustris.

    DOI: 10.1126/sciadv.aau0241

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  • BIOMIMETIC MICRO-GEL ROBOT HAVING A SOFT-RIGID HYBRID STRUCTURE

    Tomoki Watanabe, Yoshiyuki Yokoyama, Takeshi Hayakawa

    2019 20TH INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS & EUROSENSORS XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)   84 - 87   2019

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    In recent years, soft robots are attracting attention. The important point of fabrication of soft robot is mimicking soft-rigid hybrid structures like living organisms. Most of soft robots reported are in macro-scale, but soft microrobot has not been reported much. To realize soft microrobot, we propose micro-gel robots having a soft-rigid hybrid structure in this study. The soft parts are made from thermoresponsive gel actuator and it can be actuated by irradiating laser on it. The rigid parts are made of SU-8 that supports entire body of the micro-gel robot. This hybrid structure of the soft and rigid materials can realize complicated motion like living organisms. We succeeded in fabrication of the proposed micro-gel robot by using sacrificial layer process. Furthermore, we performed proof of concept of the actuation of the robot with laser irradiation.

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  • Numerical and Experimental Analyses of Three-Dimensional Unsteady Flow around a Micro-Pillar Subjected to Rotational Vibration Reviewed

    Kanji Kaneko, Takayuki Osawa, Yukinori Kametani, Takeshi Hayakawa, Yosuke Hasegawa, Hiroaki Suzuki

    Micromachines   9 ( 12 )   668   2018.12

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    The steady streaming (SS) phenomenon is gaining increased attention in the microfluidics community, because it can generate net mass flow from zero-mean vibration. We developed numerical simulation and experimental measurement tools to analyze this vibration-induced flow, which has been challenging due to its unsteady nature. The validity of these analysis methods is confirmed by comparing the three-dimensional (3D) flow field and the resulting particle trajectories induced around a cylindrical micro-pillar under circular vibration. In the numerical modeling, we directly solved the flow in the Lagrangian frame so that the substrate with a micro-pillar becomes stationary, and the results were converted to a stationary Eulerian frame to compare with the experimental results. The present approach enables us to avoid the introduction of a moving boundary or infinitesimal perturbation approximation. The flow field obtained by the micron-resolution particle image velocimetry (micro-PIV) measurement supported the three-dimensionality observed in the numerical results, which could be important for controlling the mass transport and manipulating particulate objects in microfluidic systems.

    DOI: 10.3390/mi9120668

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  • Microfluidic single-particle chemical analyzer with dual-comb coherent Raman spectroscopy Reviewed

    Takuro Ideguchi, rn|Tasuku Nakamura, rn|Shigekazu Takizawa, rn|Miu Tamamitsu, rn|Sangwook Lee, rn|Kotaro Hiramatsu, rn|Venkata Ramaiah-Badarla, rn|Jee-woong Park, rn|Yusuke Kasai, rn|Takeshi Hayakawa, rn|Shinya Sakuma, rn|Fumihito Arai, rn|Keisuke Goda

    Optics Letters   43 ( 16 )   4057 - 4060   2018.9

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  • Microfluidic single-particle chemical analyzer with dual-comb coherent Raman spectroscopy Reviewed

    Takuro Ideguchi, Tasuku Nakamura, Shigekazu Takizawa, Miu Tamamitsu, Sangwook Lee, Kotaro Hiramatsu, Venkata Ramaiah-Badarla, Jee-woong Park, Yusuke Kasai, Takeshi Hayakawa, Shinya Sakuma, Fumihito Arai, Keisuke Goda

    OPTICS LETTERS   43 ( 16 )   4057 - 4060   2018.8

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    Label-free particle analysis is a powerful tool in chemical, pharmaceutical, and cosmetic industries as well as in basic sciences, but its throughput is significantly lower than that of fluorescence-based counterparts. Here we present a label-free single-particle analyzer based on broadband dual-comb coherent Raman scattering spectroscopy operating at a spectroscopic scan rate of 10 kHz. As a proof-of-concept demonstration, we perform broadband coherent anti-Stokes Raman scattering measurements of polystyrene microparticles flowing on an acoustofluidic chip at a high throughput of >1000 particles per second. This high-throughput label-free particle analyzer has the potential for high-precision statistical analysis of a large number of microparticles including biological cells. (C) 2018 Optical Society of America

    DOI: 10.1364/OL.43.004057

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  • Parallel trapping of single motile cells based on vibration-induced flow Reviewed

    Takeshi Hayakawa, rn|Yusuke Akita, rn|Fumihito Arai

    Microfluidics and Nanofluidics   22 ( 4 )   42   2018.3

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    DOI: 10.1007/s10404-018-2062-4

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  • Three-Dimensional Blood Vessel Model with Temperature-Indicating Function for Evaluation of Thermal Damage during Surgery Reviewed

    Takeshi Hayakawa, Hisataka Maruyama, Takafumi Watanabe, Fumihito Arai

    Sensors   18 ( 2 )   345   2018.1

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    DOI: 10.3390/s18020345

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  • Fabrication of Micro-Gel Robot Having a Soft-Rigid Hybrid Structure

    Tomoki Watanabe, Yoshiyuki Yokoyama, Takeshi Hayakawa

    2018 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS)   2018

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    In recent years, soft robots attracting large attention. We aim to realize a microrobot capable of complicated movement mimicking living microorganisms. In this study, we propose a soft microgel robot that can perform peristaltic motion driven by light irradiation.

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  • On-Chip Micromanipulation Method Based on Mode Switching of Vibration-Induced Asymmetric Flow Reviewed

    Takeshi Hayakawa, rn|Fumihito Arai

    Proceedings of the 2017 IEEE International Conference on Robotics and Automation (ICRA)   6631 - 6636   2017.5

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  • The Training System Using Bionic-eye for Internal Limiting Membrane Peeling Reviewed

    Yusei Someya, Seiji Omata, Takeshi Hayakawa, Mamoru Mitsuishi, Naohiko Sugita, Kanako Harada, Yasuo Noda, Takashi Ueta, Kiyota Totsuka, Fumiyuki Araki, Hajime Aihara, Fumihito Arai

    2017 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS)   2017

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  • PARALLEL TRAPPING OF SINGLE MOTILE CELLS USING VIBRATIN-INDUCED FLOW ON MICROFLUIDIC CHIP Reviewed

    Takeshi Hayakawa, Yuusuke Akita, Fumihito Arai

    30TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2017)   1281 - 1284   2017

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    We propose an on-chip cell manipulation method for a parallel trapping of single motile cells. The proposed method enables a parallel trapping of large (&gt;= 50 mu m) motile cells, that is difficult to be achieved with conventional cell manipulation methods. We realize the trapping method by using a vibration-induced flow which is induced by applying a vibration to a microfluidic chip having microstructures on its surface. By applying a rectilinear vibration to a chip with a micropillar array, a localized flow pattern to trap single motile cells are generated around the micropillars. We succeeded in a parallel trapping of single Euglena (size is approximately 50-100 mu m) by using the proposed method. Furthermore, we demonstrated one application of the method for an evaluation of motility of motile cells with a single cell level.

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  • Ultra-high-speed on-chip cell sorting by using local flow control

    KASAI Yusuke, SAKUMA Shinya, HAYAKAWA Takeshi, ARAI Fumihito

    The Proceedings of JSME annual Conference on Robotics and Mechatronics (Robomec)   2017   2P1 - P06   2017

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    <p>We propose on-chip cell sorting system using piezo-driven dual membrane pumps. In our method, we integrate a microfluidic chip consisting of glass-Si-glass with piezoelectric actuators. The controllability and the response time of proposed flow control method are evaluated by visualizing and observing the streamline of sample flow. Then euglenas are sorted by using proposed on-chip cell sorting system. In this paper, we achieved that equivalent throughput of sorting was 50 kHz in the case of single shot and 12.5 kHz in the case of continuous sorting. In addition, we achieved 90.8% success rate and 86.4% purity of sorting, and 100% viability of euglenas after sorting.</p>

    DOI: 10.1299/jsmermd.2017.2P1-P06

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  • Design of Vibration-Induced Flow Pattern

    HAYAKAWA Takeshi, AKITA Yusuke, ARAI Fumihito

    The Proceedings of JSME annual Conference on Robotics and Mechatronics (Robomec)   2017   2P1 - P04   2017

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    <p>We propose an on-chip cell manipulation method for a parallel trapping of single motile cells. The proposed method enables a parallel trapping of large (≳ 50 μm) motile cells, that is difficult to be achieved with conventional cell manipulation methods. We realize the trapping method by using a vibration-induced flow which is induced by applying a vibration to a microfluidic chip having microstructures on its surface. By applying a rectilinear vibration to a chip with a micropillar array, a localized flow pattern to trap single motile cells are generated around the micropillars. We succeeded in a parallel trapping of single Euglena gracilis (size is approximately 50-100 μm) by using the proposed method. Furthermore, we demonstrated one application of the method for an evaluation of motility of motile cells with a single cell level.</p>

    DOI: 10.1299/jsmermd.2017.2P1-P04

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  • Surgical Training Model having Temperature Indicating Function

    HOSONO Keisuke, MARUYAMA Hisataka, SUZUKI Taishi, WATANABE Takafumi, HAYAKAWA Takeshi, TANAKA Tomohisa, ARAI Fumihito

    The Proceedings of JSME annual Conference on Robotics and Mechatronics (Robomec)   2017   1P1 - L02   2017

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    <p>This paper reports the stereoscopic model of three-dimensional blood vessel having a temperature Indicator for measurement of temperature variation during surgical training. This model consists of two hydrogel materials to mimic Young's modulus of human artery and rapid fabrication of the model using photo-polymerization. Polyethylene glycol diacrylate (PEGDA) is used for photo-crosslinkable resin and polyvinyl alcohol (PVA) is used to mimic surface properties of the blood vessel. Reversible temperature indicator is impregnated into the blood vessel model to visualize the temperature change of the model. The vessel model is fabricated by dip coating on the wax model, photo-polymerization, and lost wax method. We demonstrate color change of the fabricated vessel model by heating of the model.</p>

    DOI: 10.1299/jsmermd.2017.1P1-L02

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  • Laser-driven gel microtool for single-cell manipulation based on temperature control with a photothermal conversion material Reviewed

    T. Hayakawa, M. Kikukawa, H. Maruyama, F. Arai

    APPLIED PHYSICS LETTERS   109 ( 25 )   1 - 4   2016.12

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    We propose a laser-driven hybrid gel microtool for stable single-cell manipulation. The microtool is made of a microbead dyed with multiwalled carbon nanotubes (MWNT) and thermosensitive poly (N-isopropylacrylamide) gel coating. The gel adheres to cells at high temperatures but not at low temperatures. We can manipulate single cells without direct laser irradiation by adhering the cells to the gel on the microtool using the cell-adhesion property of the gel. The microtool is heated by trapping it with optical tweezers to make its surface cell-adhesive during the manipulation. Furthermore, we can control the optical heating property of the microtool by dyeing the microbeads with MWNT ink. The laser-heating-induced temperature increase of the microtool can be controlled from 4.2 degrees C to 23.5 degrees C by varying the concentration of MWNT ink. We succeeded in fabricating the proposed microtool and demonstrated single-cell transportation using the microtool without direct laser irradiation of the cell. Published by AIP Publishing.

    DOI: 10.1063/1.4971827

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  • Mode switching of on-chip manipulation method using vibration-induced flow for single particle loading Reviewed

    Takeshi Hayakawa|rn|Shinya Sakuma|rn|Fumihito Arai

    Proceedings of The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)   156 - 157   2016.11

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  • バイオニックアイを用いた内境界膜剥離手術トレーニングシステム

    染矢 雄成, 小俣 誠二, 早川 健, 光石 衛, 杉田 直彦, 原田 香奈子, 野田 康雄, 上田 高志, 戸塚 清人, 荒木 章之, 相原 一, 新井 史人

    日本コンピュータ外科学会誌   18 ( 4 )   323 - 324   2016.11

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  • Retinal Vessel Model Fabricated on a Curved Surface Structure for a Simulation of Microcannulation Reviewed

    Takeshi Hayakawa, ほ

    ROBOMECH Journal   3 ( 19 )   1 - 9   2016.9

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    A remarkable number of vitreoretinal surgeries are performed each year despite their difficulty. As a result, a high demand exists for a mock-up simulator of retinal vessels to simulate these surgeries. Thus, we propose an artificial retinal vessel model for simulating microcannulation surgery. Using laser lithography, polydimethylsiloxane molding, and hydraulic transfer techniques, we fabricated microchannels approximately ≃10 μm on a 24-mm-diameter curved surface structure that mimics the human eye. In the fabrication, the channel size and wall thickness were controlled to mimic a touch of retinal vessels, which gives important information for microcannulation. We succeeded in fabrication of the proposed model and liquid circulates within the microchannels of this model without leaking. Furthermore, we demonstrated a simulation of microcannulation and measurement of applied force to the model during the simulation using a force sensor placed at the bottom of the model. The results of such experiments are useful to quantitatively evaluate medical techniques.

    DOI: 10.1186/s40648-016-0059-x

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  • High-speed On-chip Local Flow Control by Synchronized Actuation of Piezo-driven Dual Membrane Pumps Reviewed

    Yusuke Kasai, Shinya Sakuma, Takeshi Hayakawa, Fumihito Arai

    2016 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS)   257   2016

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    We constructed high-speed on-chip local flow control system. The system consists of a microfluidic chip and piezoelectric actuators. The microfluidic chip has microchannels and two membrane pumps. The piezoelectric actuators are set on the membrane pumps, and we can sort cells upward and downward with synchronized actuation of piezo-driven dual membrane pumps. In this research, we demonstrate the on-chip sorting using microbeads.

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  • Training System Using Bionic-eye for Internal Limiting Membrane Peeling Reviewed

    Yusei Someya, Seiji Omata, Takeshi Hayakawa, Mamoru Mitsuishi, Naohiko Sugita, Kanako Harada, Yasuo Noda, Takashi Ueta, Kiyoto Totsuka, Fumiyuki Araki, Hajime Aihara, Fumihito Arai

    2016 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS)   67 - 68   2016

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    This paper reports on a training system for internal limiting membrane (ILM) peeling. ILM peeling task is difficult even for skilled surgeons, because ILM is a quite thin film on retina and there is a risk to damage the retina at peeling. In order to obtain the skill of ILM peeling and even evaluate newly developed medical devices, the surgical model that can be used for quantitative evaluation is highly demanded. Therefore, here we propose a surgical model for eye surgery named "Bionic-eye". Fundus of Bionic-eye has artificial ILM and retina. In addition, we assembled Bionic-eye on a whole body surgical model of human named "Bionic-humanoid".

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  • 3D Blood Vessel Model with Temperature Indicator for Evaluating Thermal Damage during Surgery Reviewed

    Takafumi Watanabe, Hisataka Maruyama, Takeshi Hayakawa, Fumihito Arai

    2016 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS)   69 - 70   2016

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    This paper presents the 3D vessel model having a temperature Indicator for evaluating thermal damage during surgery. The materials of model are polydimethylsiloxane (PDMS) and thermochromic dye which indicates color change according to temperature increase. The vessel model is fabricated by dip coating on 3D wax model and lost wax method. However, acetone for removing wax prevents the temperature responsive function of the material. Thus, the surface of the model is coated with protective layer of polyvinyl alcohol (PVA).

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  • Mechanical Characterization of Oocyte Using an Opened Microchannel Environment Reviewed

    Kou Nakahara|rn|Shinya Sakuma|rn|Takeshi Hayakawa|rn|Fumihito Arai

    Proceedings of The IEEE 26th International Symposium on Micro-NanoMechatronics and Human Science (MHS 2015).   323 - 324   2015.11

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  • Cell Adhesion Control of Optically-Driven Microtool Using Thermo-Sensitive Gel and Laser Heating Reviewed

    Maki Kikukawa, n|Takeshi Hayakawa, rn|Hisataka Maruyama, rn|Fumihito Arai

    Proceedings of The IEEE 26th International Symposium on Micro NanoMechatronics and Human Science (MHS 2015).   117   2015.11

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  • Functional Vessel Model Having Temperature Indicator for Surgical Simulator Reviewed

    Hisataka Maruyama, ほ

    Proceedings of The IEEE 26th International Symposium on Micro-NanoMechatronics and Human Science (MHS 2015).   115 - 116   2015.11

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  • 3D Rotation of Oocyte Based on Vibration-Induced Flow Control Integrated with Label-Free Polarizing Observation Reviewed

    Takeshi Hayakawa, rn|Shinya Sakuma, rn|Fumihito Arai

    Proceedings of The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)   32 - 34   2015.10

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  • On-Chip Transportation and Measurement of Mechanical Characteristics of Oocytes in an Open Environment Reviewed

    Kou Nakahara, Shinya Sakuma, Takeshi Hayakawa, Fumihito Arai

    MICROMACHINES   6 ( 5 )   648 - 659   2015.5

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    We propose a system that transports oocytes and measures their mechanical characteristics in an open environment using a robot integrated microfluidic chip (chip). The cells are transported through a micropillar array in the chip, and their characteristics are measured by a mechanical probe and a force sensor. Because the chip has an open microchannel, important cells such as oocytes are easily introduced and collected without the risk for losing them. In addition, any bubbles trapped in the chip, which degrade the measurement precision, are easily removed. To transport the oocytes through the open microchannel, we adopt a transportation technique based on a vibration-induced flow. Under this flow, oocytes arrive at the measurement point, where their mechanical characteristics are determined. We demonstrate the introduction, transportation, measurement of mechanical characteristics, and collection of oocytes using this system.

    DOI: 10.3390/mi6050648

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  • Cell Adhesion Control of Microtool Using Thermo-Responsive Polymer and Laser Heating Reviewed

    Maki Kikukawa, Takeshi Hayakawa, Hisataka Maruyama, Fumihito Arai

    2015 IEEE 15TH INTERNATIONAL CONFERENCE ON NANOTECHNOLOGY (IEEE-NANO)   77 - 80   2015

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    We proposed novel optically-driven microtool which can control cell adhesion on its surface by laser heating. The microtool is made of polystyrene microbead coated with thermo-responsive hydrogel, Poly (N-isopropylacrylamide) (PNIPAAm). Target cells adhere to the surface of the microtool at the temperature higher than Lower Critical Solution Temperature (LCST) and do not adhere at the temperature lower than LCST. Thus, the microtool can transport target cell by controlling adhesion of the cell on its surface by changing the temperature. The microtool is heated by irradiation of infrared laser used for optical tweezers and the temperature is controlled by regulating laser power and environmental temperature. First, relationship between temperature and cell adhesion rate of PNIPAAm was evaluated. Second, we experimentally measured the controllability of the temperature by temperature-sensitive fluorescent reagent. We confirmed that the temperature of the tool was increased 7.5 degrees C by irradiating the laser. Finally, we demonstrated adhesion and transportation of the single Synechocystis SPP. 6803 by irradiating laser with 65 mW.

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  • On-Chip Cell Transportation Based on Vibration-Induced Local Flow in Open Chip Environment Reviewed

    Takeshi Hayakawa, Shinya Sakuma, Fumihito Arai

    2015 IEEE/RSJ INTERNATIONAL CONFERENCE ON INTELLIGENT ROBOTS AND SYSTEMS (IROS)   1409 - 1414   2015

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    We present a novel cell manipulation method based on vibration-induced flow in open-chip environment. By applying circular vibration to a chip having micropillar patterns on its surface, local whirling flow is induced around the micropillars. Therefore, by patterning micropillar array on a chip and applying the circular vibration to the chip, we can achieve cell transportation along the array in an open chip environment. We theoretically analyzed this phenomenon with a single micropillar, and experimentally evaluated behaviours of manipulated microbeads with two micropillars. We confirm there are different manipulation modes according to the pitch of micropillars. We designed the micropillar array for an oocyte transportation based on these evaluations. We succeeded in the transportation of oocytes with up to 35.6 mu m/s and confirmed that the velocity can be changed by changing the frequency and amplitude of the applied vibration.

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  • CELL MANIPULATION METHOD BASED ON VIBRATION-INDUCED LOCAL FLOW CONTROL IN OPEN CHIP ENVIRONMENT Reviewed

    Takeshi Hayakawa, Shinya Sakuma, Fumihito Arai

    2015 28TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2015)   200 - 203   2015

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    We present a novel cell manipulation method using vibration-induced local flow in open chip environment. By applying circular vibration to micropillars on a chip, local whirling flow is induced around the micropillars. From the observation of this unique phenomenon, we propose the concept of cell manipulation in open chip environment. We analyze this phenomenon theoretically, and evaluate the effect of the frequency and the amplitude of applied vibration. We design the micropillar array according to the analysis for transportation for oocytes. We apply the proposed method to transportation of mouse oocytes and confirm that the velocity of transportation is approximately 25 mu m/s.

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  • A Single Cell Extraction Chip Using Vibration-Induced Whirling Flow and a Thermo-Responsive Gel Pattern Reviewed

    Takeshi Hayakawa, Shinya Sakuma, Takeshi Fukuhara, Yoshiyuki Yokoyama, Fumihito Arai

    MICROMACHINES   5 ( 3 )   681 - 696   2014.9

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    We propose a single cell extraction chip with an open structure, which utilizes vibration-induced whirling flow and a single cell catcher. By applying a circular vibration to a micropillar array spiral pattern, a whirling flow is induced around the micropillars, and target cells are transported towards the single cell catcher placed at the center of the spiral. The single cell catcher is composed of a single-cell-sized hole pattern of thermo-responsive gel. The gel swells at low temperatures (less than or similar to 32 degrees C) and shrinks at high temperatures (greater than or similar to 32 degrees C), therefore, its volume expansion can be controlled by an integrated microheater. When the microheater is turned on, a single cell is trapped by the hole pattern of the single cell catcher. Then, when the microheater is turned off, the single cell catcher is cooled by the ambient temperature. The gel swells at this temperature, and the hole closes to catch the single cell. The caught cell can then be released into culture wells on a microtiter plate by heating the gel again. We conducted single cell extraction with the proposed chip and achieved a 60% success rate, of which 61% cells yielded live cells.

    DOI: 10.3390/mi5030681

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  • Fabrication of an On-Chip Nanorobot Integrating Functional Nanomaterials for Single-Cell Punctures Reviewed

    Takeshi Hayakawa, Shota Fukada, Fumihito Arai

    IEEE TRANSACTIONS ON ROBOTICS   30 ( 1 )   59 - 67   2014.2

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    Cell manipulations and cell surgeries are key techniques in biotechnology today. Micro/nanorobots integrated on a microfluidic chip (on-chip robot) are a promising technology for cell manipulations and cell surgeries because of their operator skill independency and robustness for external environments. These features enable high-throughput cell manipulations and cell surgeries on a microfluidic chip. However, it is difficult to apply previous on-chip robots for small cells of order approximate to 10 mu m because the manipulation or surgery probes of those robots are a few micrometers in size. This size has been restricted by their fabrication, employing a standard mask lithography process. We fabricated on-chip robots of nanometer size by femtosecond laser exposure (nanorobots). The processing resolutions were 270 nm (linewidth) and 600 nm (thickness). Furthermore, our fabrication technique enabled the nanorobot to have a hybrid structure integrating functional nanomaterials (hybrid nanorobot). By integrating the various functional nanomaterials on the nanorobot, we can create a new function for the nanorobot. In this study, we fabricated a hybrid nanorobot with carbon nanotubes (CNTs) of high photothermal efficiency. We demonstrated a single-cell puncture with this nanorobot by irradiating the CNTs with an infrared laser and generating heat at that point. Additionally, we demonstrated an optical manipulation of the nanorobot that makes it possible to perform a cell puncture with high spatial flexibility and high positioning accuracy.

    DOI: 10.1109/TRO.2013.2284402

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  • SINGLE CELL SEPARATION BY USING ACCESSIBLE MICROFLUIDIC CHIP Reviewed

    Takeshi Hayakawa, Takeshi Fukuhara, Keitaro Ito, Fumihito Arai

    2014 IEEE 27TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS)   1035 - 1038   2014

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    Here we proposed a novel device named CACS (Convective Accumulation-based Cell Separator) system that is composed of a cell-trapper unit with accessible (open-structured) microfluidic device and a cell-picker device with high aspect ratio PDMS pillars. Utilizing this systematic instrument, we demonstrated proof-of-concept for cell separation through the process of (1) single cell trap in a well (2) single cell picking by pillar-based tweezers and (3) cell recovery. The optimized system for mammalian cells had enabled us to trap-in and pick-up target cells in 100% efficiency as far as tested. Moreover, picked cells showed 74.5% viability, as a result of a less-invasive cell separation technique. Furthermore, a series of different PDMS pillars indicated their varied efficiency in pick-up and recovery steps. We also found out that the aspect ratio of PDMS pillar is critical for tweezing a cell of interest. To improve on the performance of CACS system, further tuning is necessary, especially on the recovery unit for achieving an efficient and damage-free separation. Though CACS system shows low-throughput at this moment, its simplicity is applicable for any cell type by optimizing each pipeline.

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  • On-Chip Cell Manipulation by Vibration-Induced Whirling Flow Reviewed

    Takeshi Hayakawa, Shinya Sakuma, Fumihito Arai

    2014 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS)   196   2014

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    We present a cell manipulation method by using vibration induced whirling flow. The local flow is induced just around a micropillar by applying circular vibration to the micropillar. By patterning a micropillar array on a chip and applying circular vibration to the chip, flow for cell transport can be generated along the array. As an application, we demonstrate a single cell extraction using the vibration-induced flow and single cell catcher made by thermo-responsive gel. Target cells are transported to the single cell catcher by vibration induced flow and caught by the single cell catcher. We tried 30 times single cell extraction, succeeded in 100% single cell catch and 60% extraction to external culture well.

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  • 3A1-L05 Single Cell Separation Device by Using Bioresist(Nano/Micro Manipulation System)

    HAYAKAWA Takeshi, FUKUHARA Takeshi, YOKOYAMA Yoshiyuki, ARAI Fumihito

    The Proceedings of JSME annual Conference on Robotics and Mechatronics (Robomec)   2014   _3A1-L05_1 - _3A1-L05_3   2014

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    We propose a simple and low cost single cell separation device by using a thermoresponsive gel called "Bioresist". Proposed device is consist of a microheater and microhole pattern of Bioresist (Cell catcher), and it can be fabricated by a standard MEMS fabrication process. Cell catcher can shrink and swell by heating and cooling the medium, and hold a target cell when it swells. For single cell separation, only the device is needed and it is possible to realize single cell separation with very low initial cost. In this paper, we report feasibility study of single cell separation and dispense with proposed device. We succeeded in single cell separation with 90 % success rate and single cell dispense with 15 % success rate.

    DOI: 10.1299/jsmermd.2014._3a1-l05_1

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  • Single Cell Puncture with Optically Manipulated Hybrid Nanorobot Reviewed

    Takeshi Hayakawa|rn|Fumihto Arai

    Proceedings of The 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)   1042 - 1044   2013.10

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  • Fabrication and Manipulation of 3D Hybrid Nanorobot For Single Cell Puncture Reviewed

    Takeshi Hayakawa, Fumihito Arai

    2013 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS)   291 - 292   2013

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    We proposed hybrid nanorobot that can be manipulated by optical tweezers and has hybrid structure integrating functional nanomaterials. We employed femtosecond laser exposure for fabrication of the nanorobot having 3D structure with nanometric size and integrating functional nanomaterials. We succeeded in fabricating nanorobot with carbon nanotubes (CNTs) that has a high photothermal efficiency. We demonstrated single cell puncture with the nanorobot by irradiating infrared (IR) laser to CNT and generating heat on that point. The puncture shape was dependent on irradiated lase power. Furthermore, we evaluated optical manipulation characteristics of the nanorobot.

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  • High Thermal Conductive Nano Pillars for Temperature Distribution Measurement of a Single Cell Reviewed

    Takeshi Hayakawa, Hisataka Maruyama, Fumihito Arai

    2012 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE (MHS)   488 - 490   2012

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    Single cell analysis is attracting large attention because that makes possible more quantitative and detailed analysis compared to conventional cell analysis. In this study, we proposed single cell temperature measurement devise by using high thermal conductive nano pillars and temperature sensitive fluorescent reagent. We fabricated the temperature measurement device and performed temperature calibration, finite element analysis and cell incubation on the device.

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  • Photo-Assisted Electronic Transport in Impurity-Doped Carbon Nanotubes Reviewed

    Daisuke Hirai, Takeshi Hayakawa, Satoru Konabe, Kazuyuki Watanabe, Takahiro Yamamoto

    JAPANESE JOURNAL OF APPLIED PHYSICS   48 ( 8 )   1 - 4   2009.8

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    We report a theoretical study on the electronic structures and photo-assisted transport properties of three types of impurity-doped carbon nanotubes: boron or nitrogen substituted and pyridine-like nitrogen-doped. The pyridine-like nitrogen-doped carbon nanotubes exhibit a nearly flat impurity band between the valence and the conduction bands, whereas the boron- and nitrogen-substituted nanotubes show dispersive impurity bands overlapping the valence and the conduction bands, respectively. Due to the existence of the flat band, the pyridine-like nitrogen-doped carbon nanotubes show unipolar transport and their conductances are enhanced by increasing the power and frequency of the light. (C) 2009 The Japan Society of Applied Physics

    DOI: 10.1143/JJAP.48.08JB02

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Books

  • Light Robotics - Structure-mediated Nanobiophotonics

    Takeshi Hayakawa, Hisataka Maruyama, rn|Fumihito Arai( Role: Joint author2-7. Optically Driven Micro- and Nano- Robots)

    Elsevier  2017.5 

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    Total pages:482   Responsible for pages:44   Language:English   Book type:Scholarly book

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  • 細胞の特性計測・操作と応用

    早川 健, 新井 史人( Role: Joint author担当:光による操作(2.1 pp.87-109)/音響流れによる操作(2.3 pp.126-146))

    コロナ社  2016.12 

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    Total pages:270   Responsible for pages:44   Language:Japanese   Book type:Scholarly book

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  • LIGHT DRIVING OF ON-CHIP MICRO GEL ACTUATOR TOWARDS MASSIVE INTEGRATION

    Yuha Koike, Yoshiyuki Yokoyama, Takeshi Hayakawa

    2019 20TH INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS & EUROSENSORS XXXIII (TRANSDUCERS & EUROSENSORS XXXIII)   728 - 731   2019

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    In this paper, we present driving method of on-chip hydrogel microactuator by using light irradiation. The presented driving method enables local heating of temperature responsive hydrogel actuator. The hydrogel actuator is made of temperature responsive gel and fabricated on a microfluidic chip with light absorbing micropattern. Thus, the actuators can be driven by irradiating light on it and increase its temperature. The advantage of this driving method is it can achieve local heating of the actuator and reducing heat dissipation to substrate and environment. Furthermore, the local heating enables to put the multiple actuators in short distance without unintended actuation of neighbor actuators by heat dissipation. Additionally, heating with light irradiation does not need any wiring on the chip. Therefore, proposed driving method enables to integrate large number of hydrogel actuators on a chip and independently drive it. This massive integration of large number of on-chip hydrogel microactuators will realize high-throughput and highly functional various single cell manipulations on a microfluidic chip.

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  • 内境界膜剥離手術トレーニング用眼球モデルの作成

    高尾宗之, 荒木章之, 戸塚清人, 上田高志, 野田康雄, 相原一, 染矢雄成, 小俣誠二, 早川健, 新井史人, 原田香奈子, 光石衛

    日本眼科学会雑誌   121 ( 臨増 )   300 - 300   2017.3

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  • Training system for retinal surgery with bionic eye model

    Seiji OMATA, Yusei SOMEYA, Shun’ya ADACHI, Takeshi HAYAKAWA, Kanako HARADA, Mamoru MITSUISHI, Kiyoto TOTSUKA, Fumiyuki ARAKI, Muneyuki TAKAO, Hajime AIHARA, Fumihito ARAI

    バイオメカニクス研究センター&エレクトロニクス実装学会九州支部合同研究会   2017.3

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  • Polymer-based blood vessel models with micro-temperature sensors in Eve

    Mizue Mizoshiri, Yasuaki Ito, Takeshi Hayakawa, Hisataka Maruyama, Junpei Sakurai, Seiichi Ikeda, Fumihito Arai, Seiichi Hata

    Proceedings of SPIE - The International Society for Optical Engineering   10167   2017

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    Cu-based micro-temperature sensors were directly fabricated on poly(dimethylsiloxane) (PDMS) blood vessel models in EVE using a combined process of spray coating and femtosecond laser reduction of CuO nanoparticles. CuO nanoparticle solution coated on a PDMS blood vessel model are thermally reduced and sintered by focused femtosecond laser pulses in atmosphere to write the sensors. After removing the non-irradiated CuO nanoparticles, Cu-based microtemperature sensors are formed. The sensors are thermistor-type ones whose temperature dependences of the resistance are used for measuring temperature inside the blood vessel model. This fabrication technique is useful for direct-writing of Cu-based microsensors and actuators on arbitrary nonplanar substrates.

    DOI: 10.1117/12.2265050

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  • バイオニックヒューマノイド:内境界膜剥離術手術訓練のための眼球モデル

    小俣誠二, 染矢雄成, 足達俊哉, 早川健, 原田香奈子, 原田香奈子, 光石衛, 戸塚清人, 荒木章之, 高尾宗之, 相原一, 新井史人

    日本機械学会ロボティクス・メカトロニクス講演会講演論文集(CD-ROM)   2017   2017

  • フェムト秒レーザーを用いた超高速細胞分取システム

    飯野敬矩, 萩原宏規, 前野貴則, LEE S. W., 笠井宥佑, 佐久間臣耶, 早川健, 新井史人, 細川陽一郎

    応用物理学会春季学術講演会講演予稿集(CD-ROM)   64th   2017

  • フェムト秒レーザー誘起衝撃力を用いたオープンチップ中の高速流体からの細胞分取

    飯野敬矩, 飯野敬矩, 山川健, 山川健, 萩原宏規, 萩原宏規, 佐久間臣耶, 佐久間臣耶, 早川健, 早川健, 益田泰輔, 益田泰輔, 新井史人, 新井史人, 細川陽一郎, 細川陽一郎

    応用物理学会春季学術講演会講演予稿集(CD-ROM)   63rd   2016

  • 外部駆動高速アクチュエータを統合した局所的オンチップ流体制御

    笠井宥佑, 佐久間臣耶, 早川健, 新井史人

    日本ロボット学会学術講演会予稿集(CD-ROM)   34th   2016

  • 振動誘起流れのモード切替による微小構造体のオンチップ運動制御

    早川健, 佐久間臣耶, 新井史人

    化学とマイクロ・ナノシステム学会研究会講演要旨集   33rd   2016

  • 超高速局所流体制御を用いたオンチップセルソーティングシステム

    佐久間臣耶, 笠井宥佑, 早川健, 新井史人

    化学とマイクロ・ナノシステム学会研究会講演要旨集   34th   2016

  • オンチップロボティクスが切り拓く世界

    伊藤啓太郎, 佐久間臣耶, 早川健, 新井史人

    化学とマイクロ・ナノシステム   15 ( 1 )   2016

  • 多層マイクロ流路を用いた細胞3Dフォーカシング

    笠井宥佑, 佐久間臣耶, 早川健, 新井史人

    計測自動制御学会システムインテグレーション部門講演会(CD-ROM)   17th   2016

  • 28pm3-B-6 3D Vessel Model Having Temperature Indicator for Surgical Simulator

    MARUYAMA Hisataka, WATANABE Takafumi, Tanaka Tomohisa, HAYAKAWA Takeshi, ARAI Fumihito

    2015 ( 7 )   "28pm3 - B-6-1"-"28pm3-B-6-2"   2015.10

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    This paper reports the 3D vessel model having temperature history indicator for surgical simulator. The temperature indicator consists of polydimethylsiloxane (PDMS) and thermochromic pigment presenting non-reversible color change according to temperature increase. The indicator has transparency to observe the catheter inside the model during simulation. Distribution of maximum increased temperature is calculated from the color change of the indicator using image analysis. In the range of the indicator is from 50 to 94 degree of C, the accuracy of the temperature measurement is approximately 2.0 degrees of C. The renal artery model having temperature sensor was fabricated by rapid prototyping of wax mold, dip-coating of temperature indicator, and remove of was mold.

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  • 1P1-O09 Cell Manipulation in Open-Chip Environment Based on Vibration-Induced Flow

    HAYAKAWA Takeshi, SAKUMA Shinya, ARAI Fumihito

    2015   "1P1 - O09(1)"-"1P1-O09(4)"   2015.5

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    We present a novel cell manipulation method based on vibration-induced flow in open-chip environment. By applying circular vibration to a chip having micropillars, local flow is induced around the micropillars. Therefore, by patterning micropillar array on the chip and applying the circular vibration, we can achieve cell transportation along the array in the open chip environment. We theoretically analyzed this phenomenon with a single micropillar, and experimentally evaluated behaviors of manipulated microbeads with two micropillars. We confirmed there are different manipulation modes according to the pitch of micropillars. We designed the micropillar array for transportation of mouse oocytes based on these evaluations. We succeeded in transportation of oocytes with up to 35.6 μm/s and confirmed that the transportation velocity can be changed by changing the frequency and amplitude of the applied vibration.

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  • 1A1-X07 Transportation of oocyte using vibration-induced flow and measurement of mechanical characteristics

    Nakahara Kou, Sakuma Shinya, Hayakawa Takeshi, Arai Fumihito

    2015   "1A1 - X07(1)"-"1A1-X07(3)"   2015.5

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    When high-frequency circular vibration is applied to a micropillar in fluid, flow is generated around the micropillar. In this research, we propose an innovative system of oocyte transportation by using vibration-induced flow. It is integrated on a chip with on-chip probe and force sensor for mechanical characterization of oocyte. This system enables us to measure the mechanical property of oocyte in open chip environment. As a result, the bubbles on the chip can be eliminated easily. Moreover, loading and picking up of oocytes is easily done by using conventional mechanical micromanipulator. In this paper, we constructed on-chip transportation and mechanical characterization of oocyte, and demonstrated effectiveness of our proposed method by experiments.

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  • 化学とマイクロ・ナノシステム学会誌 Vol.14, No.1 (2015)

    2015.3

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  • 振動誘起流れを用いたオンチップ卵子回転操作

    早川健, 佐久間臣耶, 新井史人

    日本ロボット学会学術講演会予稿集(CD-ROM)   33rd   2015

  • 振動誘起流れを用いた細胞のオンチップ搬送・回転制御

    早川健, 佐久間臣耶, 新井史人

    化学とマイクロ・ナノシステム   14 ( 1 )   2015

  • オープンなマイクロ流路環境を利用した卵子の機械的特徴量計測

    中原康, 佐久間臣耶, 早川健, 新井史人

    日本ロボット学会学術講演会予稿集(CD-ROM)   33rd   2015

  • 振動誘起流れを用いた細胞のオンチップ搬送・回転制御

    早川健, 佐久間臣耶, 新井史人

    化学とマイクロ・ナノシステム学会研究会講演要旨集   30th   2014

  • 振動誘起流れを用いたオンチップ細胞操作

    早川健, 佐久間臣耶, 新井史人

    日本ロボット学会学術講演会予稿集(CD-ROM)   32nd   2014

  • 7AM2-C-6 Thermal gel actuator by using Bioresist

    ITO K., HAYAKAWA T., SAKUMA S., YOKOYAMA Y., ARAI F.

    2013 ( 5 )   249 - 250   2013.11

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    We propose an innovative simple and smart microfluidic actuator, which is made of photoprocessible thermoresponsible gel, for the disposable biomedical application. The smart gel is called "Bioresist", and arbitraly micropatterns can be achieved by the standard photolithography technique. The actuator is actuated only by local heat control of microheater, and several microfluidic functions are achieved by its expansion/contracting motion. We would remark that the required voltage which can control the expanding/contracting motion is only about 1.5 V. Furthermore, proposed method is easy to integrate into microfluidic chip.

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  • 1A1-O08 Photo Fabrication of 3D Nanorobot with Nanometric Probe(Nano/Micro Manipulation System)

    HAYAKAWA Takeshi, FUKADA Shota, ARAI Fumihito

    2013   "1A1 - O08(1)"-"1A1-O08(4)"   2013.5

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    In this paper, we proposed a 3D hybrid nanorobot for a single cell surgery including functional nanomaterials. We succeeded in fabricating the 3D hybrid nanorobot with a carbon nanotube (CNT) by using femtosecond laser exposure. CNT has high-efficient photothermal characteristics and it can be used for a cell puncture. As a feasibility study of the proposed single cell puncture, we performed the single cell puncture with this nanorobot by irradiating an IR laser on the CNT. The target cell was partially punctured with the size of approximately 10μm. We can realize the arbitrary local cell puncture with this hybrid nanorobot.

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Presentations

  • 振動誘起流れのデザイン:運動性細胞の並列補足制御

    早川 健, rn|秋田 祐甫, 新井 史人

    日本機械学会ロボティクス・メカトロニクス講演会 (ROBOMECH2017)  2017.5 

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  • 局所流体制御による超高速オンチップ細胞ソーティング

    笠井宥佑, rn|佐久間臣耶, rn|早川健, 新井史人

    日本機械学会ロボティクス・メカトロニクス講演会 (ROBOMECH2017)  2017.5 

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  • バイオニックヒューマノイド:内境界膜剝離術手術訓練のための眼球モデル

    小俣 誠二, rn|染矢 雄成, rn|早川 健, rn|原田, 香奈子, rn|光石 衛, rn|戸塚 清人, rn|荒木 章之, rn|相原 一, 新井 史人

    日本機械学会ロボティクス・メカトロニクス講演会 (ROBOMECH2017)  2017.5 

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  • On-Chip Micromanipulation Method Based on Mode Switching of Vibration-Induced Asymmetric Flow International conference

    Takeshi Hayakawa, rn|Fumihito Arai

    Proceedings of the 2017 IEEE International Conference on Robotics and Automation (ICRA)  2017.5  IEEE

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  • Parallel Trapping of Single Motile Cells Using Vibration-Induced Flow on Microfluidic Chip International conference

    Takeshi Hayakawa, rn|Yuusuke Akita, rn|Fumihito Arai

    Proceedings of The 30th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2017).  2017.1  IEEE

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  • 多層マイクロ流路を用いた細胞3Dフォーカシング

    笠井 宥佑, 佐久間, 臣耶, n|早川 健, 新井 史人

    第17回計測自動学会 システムインテグレーション部門講演会  2016.12 

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  • 温度応答型三次元⾎管モデルを⽤いた温度履歴計測

    渡邉 貴文, rn|丸山 央峰, 早川 健, 新井史人

    第2回 日本機械学会イノベーション講演会  2016.11 

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  • High-speed On-chip Local Flow Control by Synchronized Actuation of Piezo-driven Dual Membrane Pumps International conference

    Yusuke Kasai|rn|Shinya Sakuma|rn|Takeshi Hayakawa|rn|Fumihito Arai

    Proceedings of The IEEE 27th International Symposium on Micro-NanoMechatronics and Human Science (MHS 2016).  2016.11  IEEE

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  • Training System Using Bionic-eye for Internal Limiting Membrane Peeling International conference

    Yusei Someya, ほ

    Proceedings of The IEEE 27th International Symposium on Micro-NanoMechatronics and Human Science (MHS 2016).  2016.11  IEEE

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  • 3D Blood Vessel Model with Temperature Indicator for Evaluating Thermal Damage during Surgery International conference

    Takafumi Watanabe|rn|Hisataka Maruyama|rn|Takeshi Hayakawa|rn|Fumihito Arai

    Proceedings of The IEEE 27th International Symposium on Micro-NanoMechatronics and Human Science (MHS 2016).  2016.11  IEEE

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  • Mode switching of on-chip manipulation method using vibration-induced flow for single particle loading International conference

    Takeshi Hayakawa|rn|Shinya Sakuma|rn|Fumihito Arai

    Proceedings of The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)  2016.11  Proceedings of The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)

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  • 振動誘起流れを用いた運動性細胞の並列トラップ操作

    早川 健, rn|秋田 祐甫, 新井 史人

    第34回日本ロボット学会学術講演会  2016.9 

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  • 外部駆動高速アクチュエータを統合した局所的オンチップ流体制御

    笠井 宥佑, 佐久間, 臣耶, n|早川 健, 新井 史人

    第34回日本ロボット学会学術講演会  2016.9 

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  • 超高速局所流体制御を用いたオンチップセルソーティングシステム

    佐久間 臣耶, rn|笠井 宥佑, n|早川 健, 新井 史人

    化学とマイクロ・ナノシステム学会|rn|第34回研究会  2016.9 

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  • 熱損傷評価のための温度応答型三次元血管モデル

    渡邉 貴文

    日本機械学会 ロボティクス・メカトロニクス講演会2016(Robomech2016)  2016.6 

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  • 振動誘起流れのモード切替による微小構造体のオンチップ運動制御

    早川 健, 佐久間, 臣, 新井史人

    化学とマイクロ・ナノシステム学会第33回研究会  2016.4 

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  • フェムト秒レーザー誘起衝撃力を用いたオープンチップ中の高速流体からの細胞分取

    飯野 敬矩

    第63回応用物理学会春季学術講演会  2016.3 

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  • 温度履歴表示機能を有する手術シミュレーション用血管モデル

    渡邉 貴文, rn|丸山 央峰, 早川 健, 新井史人

    第1回 日本機械学会イノベーション講演会  2015.11 

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  • Mechanical Characterization of Oocyte Using an Opened Microchannel Environment International conference

    Kou Nakahara|rn|Shinya Sakuma|rn|Takeshi Hayakawa|rn|Fumihito Arai

    Proceedings of The IEEE 26th International Symposium on Micro-NanoMechatronics and Human Science (MHS 2015).  2015.11  IEEE

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  • Cell Adhesion Control of Optically-Driven Microtool Using Thermo-Sensitive Gel and Laser Heating International conference

    Maki Kikukawa, n|Takeshi Hayakawa, rn|Hisataka Maruyama, rn|Fumihito Arai

    Proceedings of The IEEE 26th International Symposium on Micro NanoMechatronics and Human Science (MHS 2015).  2015.11  IEEE

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  • Functional Vessel Model Having Temperature Indicator for Surgical Simulator International conference

    Hisataka Maruyama, ほ

    Proceedings of The IEEE 26th International Symposium on Micro-NanoMechatronics and Human Science (MHS 2015).  2015.11  IEEE

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  • 手術シミュレータのための温度履歴計測機能を有する3次元血管モデル

    丸山央峰, ほ

    日本機械学会 第7回マイクロ・ナノ工学シンポジウム  2015.10 

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  • 3D Rotation of Oocyte Based on Vibration-Induced Flow Control Integrated with Label-Free Polarizing Observation International conference

    Takeshi Hayakawa, rn|Shinya Sakuma, rn|Fumihito Arai

    Proceedings of The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)  2015.10  The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)

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  • 振動誘起流れを用いたオンチップ卵子回転操作

    早川 健, 佐久間, 臣, 新井史人

    第33回日本ロボット学会学術講演会  2015.9 

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  • オープンなマイクロ流路環境を利用した卵子の機械的特徴量計測

    中原 康, r, 佐久間, 臣耶, 早川 健, 新井史人

    第33回日本ロボット学会学術講演会  2015.9 

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  • On-Chip Cell Transportation Based on Vibration-Induced Local Flow in Open Chip Environment International conference

    Takeshi Hayakawa, rn|Shinya Sakuma, rn|Fumihito Arai

    Proceedings of The 2015 IEEE/RSJ International Conference on Intelligent Robots and Systems (IROS 2015)  2015.9  IEEE Robotics and Automation Society

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  • Cell Adhesion Control of Microtool Using Thermo-Responsive Polymer and Laser Heating International conference

    Maki Kikukawa, n|Takeshi Hayakawa, rn|Hisataka Maruyama, rn|Fumihito Arai

    Proceedings of The 15th International Conference on Nanotechnology (IEEE NANO 2015)  2015.7  IEEE Nanotechnology Council

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  • 温度応答性ポリマーを用いたマイクロツール表面の細胞接着制御

    菊川 真希, rn|早川 健, 丸山 央峰, 新井 史人

    日本機械学会 ロボティクス・メカトロニクス講演会2015(Robomech2015)  2015.5 

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  • 振動誘起流れを用いたオープンチップでの細胞操作

    早川 健, 佐久間, 臣, 新井史人

    日本機械学会 ロボティクス・メカトロニクス講演会2015(Robomech2015)  2015.5 

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  • 振動誘起流れによる卵子の搬送と機械的特徴量計測

    中原 康, r, 佐久間, 臣耶, 早川 健, 新井史人

    日本機械学会 ロボティクス・メカトロニクス講演会2015(Robomech2015)  2015.5 

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  • Cell manipulation method based on vibration-induced local flow control in open chip environment International conference

    Takeshi Hayakawa, rn|Shinya Sakuma|rn|Fumihito Arai

    Proceesings of The 28th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2015).  2015.1  IEEE

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  • On-Chip Cell Manipulation by Vibration-Induced Whirling Flow International conference

    Takeshi Hayakawa|rn|Shinya Sakuma|rn|Fumihito Arai

    Proceesings of The IEEE 25th International Symposium on Micro-Nano Mechatronics and Human Science (MHS 2014).  2014.11  IEEE

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  • 振動誘起流れを用いた細胞のオンチップ搬送・回転制御

    早川 健, 佐久間, 臣, 新井史人

    化学とマイクロ・ナノシステム学会第30回研究会  2014.10 

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  • 振動誘起流れを用いたオンチップ細胞操作

    早川 健, 佐久間, 臣, 新井史人

    第32回日本ロボット学会学術講演会  2014.9 

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  • バイオレジストを用いた単一細胞分離デバイス

    早川 健, rn|福原 武志, 横山 義之, 新井史人

    日本機械学会 ロボティクス・メカトロニクス講演会2014(Robomech2014)  2014.5 

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  • Single Cell Separation by Using Accessible Microfluidic Chip International conference

    Takeshi Hayakawa|r, Takeshi Fukuhara|rn|Keitaro Ito|rn|Fumihto Arai

    Proceedings of The 27th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2014).  2014.1  IEEE

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  • 機能性ナノマテリアルを搭載した3次元光駆動ハイブリッドナノロボット

    早川 健, 新井 史人

    化学とマイクロ・ナノシステム学会第28回研究会  2013.12 

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  • バイオレジストを用いたゲルアクチュエータ

    伊藤 啓太郎

    日本機械学会 第5回マイクロ・ナノ工学シンポジウム  2013.11 

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  • Fabrication and Manipulation of 3D Hybrid Nanorobot For Single Cell Puncture International conference

    Takeshi Hayakawa|rn|Fumihto Arai

    Proceedings of The IEEE 24th International Symposium on Micro-Nano Mechatronics and Human Science (MHS 2013)  2013.11  IEEE

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  • Single Cell Puncture with Optically Manipulated Hybrid Nanorobot International conference

    Takeshi Hayakawa|rn|Fumihto Arai

    Proceedings of The 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)  2013.10  The 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)

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  • ナノプローブを有するナノロボットの3次元光創製

    早川 健, rn|深田 翔太, 新井 史人

    日本機械学会 ロボティクス・メカトロニクス講演会2013(Robomech2013)  2013.5 

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  • High Thermal Conductive Nano Pillars for Temperature Distribution Measurement of a Single Cell International conference

    Takeshi Hayakawa|r, Hisataka Maruyama|rn|Fumihto Arai

    Proceedings of The IEEE 23rd International Symposium on Micro-Nano Mechatronics and Human Science (MHS 2012)  2012.11  IEEE

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  • 高熱伝導ナノピラーを有する単一細胞温度計測デバイス

    早川 健, rn|丸山 央峰, 新井 史人

    日本ロボット学会 第30回記念 学術講演会  2012.9 

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  • Photoconductance of Carbon Nanotubes: NEGF+DFT Calculations

    Takeshi Hayakawa|rn|Daisuke Hirai|rn|Takahiro Yamamoto|rn|Kazuyuki Watanabe

    International Symposium on Surface Science and Nanotechnology (ISSS-5)  2008.11 

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  • 光照射による半導体CNTの電気伝導制御

    早川健, rn|山本貴博, 渡辺一之

    日本物理学会2008年秋季大会  2008.9 

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  • カーボンナノチューブの光誘起電気伝導計算

    早川 健, rn|山本 貴博, 渡辺 一之

    日本物理学会第63回年次大会  2008.3 

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Awards

  • 令和5年度科学技術分野の文部科学大臣表彰 若手科学者賞

    2023.4   文部科学省   マイクロ/ナノスケール物理現象に基づく微細操作技術の研究

    早川 健

  • 優秀講演論文賞

    2022.12   日本機械学会マイクロ・ナノ工学部門   空圧駆動マイクロピラーデバイスによる振動誘起流れの生起制御

    佐藤 拓, 金子 完治, 早川 健, 鈴木 宏明

  • CM Ho best paper in Micro/Nanofluidics

    2022.4   The 17th IEEE International Conference on Nano/Micro Engineered & Molecular Systems (IEEE-NEMS 2022)   Detection of Nanoparticles in A Minute Sample Using the Vibration Induced Flow

  • Best Paper Awards

    2021.12   32nd 2021 International Symposium on Micro-NanoMechatronics and Human Science (From Micro & Nano Scale Systems to Robotics & Mechatronics Systems)   Evaluation of Dynamic Response of Water-pre-stretched Dielectric Elastomer Actuator

    Asahi Tsujino, Takeshi Hayakawa

  • ROBOMECH表彰 (学術研究分野)

    2021.6   日本機械学会ロボティクス・メカトロニクス部門   振動誘起流れを用いたマイクロミキサーのPIVによる性能評価

    松井 駿幸, 鈴木 宏明, 早川 健

  • ケミナス若手優秀賞

    2020.5   化学とマイクロ・ナノシステム学会   振動誘起流れを用いたオンチップ細胞操作手法の提案

    早川 健

  • 日本コンピュータ外科学会 2016年度講演論文賞

    2017.10   日本コンピュータ外科学会   バイオニックアイを用いた内境界膜剥離手術トレーニングシステム

    染矢雄成, rn|小俣誠二, n|早川健, n|光石衛, n|杉田直彦, n|原田香奈子, rn|野田康雄, n|上田隆, n|戸塚清人, n|荒木章之, n|相原一, 新井史人

  • Best Paper Award

    2016.11   IEEE 27th International Symposium on Micro-NanoMechatronics and Human Science (MHS2016)   High-speed On-chip Local Flow Control by Synchronized Actuation of Piezo-driven DualMembrane Pumps

  • 最優秀研究賞

    2016.9   化学とマイクロ・ナノシステム学会第34回研究会   超高速局所流体制御を用いたオンチップセルソーティングシステム

  • IEEE Robotics and Automation Society Japan Chapter Young Award

    2015.10   IEEE Robotics and Automation Society Japan Chapter   On-Chip Cell Transportation Based on Vibration-Induced Local Flow in Open Chip Environment

  • 日本ロボット学会研究奨励賞

    2015.9   日本ロボット学会   振動誘起流れを用いたオンチップ細胞操作

  • IEEE Nagoya Section Conference Presentation Award

    2015.4   IEEE Nagoya Section   Cell Manipulation Method Based on Vibration-Induced Local Flow Control in Open Chip Environment

  • 優秀ポスター賞

    2014.10   化学とマイクロ・ナノシステム学会第30回研究会   振動誘起流れを用いた細胞のオンチップ搬送・回転制御

  • Best Poster Award

    2013.11   IEEE 24th International Symposium on Micro-NanoMechatronics and Human Science (MHS2013)   Fabrication and Manipulation of 3D Hybrid Nanorobot For Single Cell Puncture

  • 第三回計算科学フロンティアフォーラム|rn|学生ポスターセッション 大学別最優秀賞

    2008.9   計算科学フロンティアフォーラム   光照射による半導体CNTの電気伝導制御

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Research Projects

  • 生体内模擬環境を利用した細胞内CAの遠隔制御評価

    Grant number:JPMJMS2217-6-1  2022.12 - 2026.3

    科学技術振興機構  ムーンショット型研究開発事業  中央大学

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  • ワイドレンジ周波数機械振動を用いたマルチスケール微細操作システムの創製

    Grant number:22H01454  2022.4 - 2025.3

    日本学術振興会  科学研究費助成事業  基盤研究(B)  中央大学

    早川 健, 鈴木 宏明, 工藤 謙一

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    Grant amount: \17810000 ( Direct Cost: \13700000 、 Indirect Cost: \4110000 )

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  • 合体・分解型多機能マイクロゲルロボットの創製

    Grant number:21K18703  2021.7 - 2023.3

    日本学術振興会  科学研究費助成事業  挑戦的研究(萌芽)  中央大学

    早川 健

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    Grant amount: \6370000 ( Direct Cost: \4900000 、 Indirect Cost: \1470000 )

    本研究では,マイクロゲルをソフトアクチュエータとして搭載したロボットに,分子認識機能を利用した合体・分解機能を付与し,単純な構造のロボットを合体させることで多機能を創発する「合体・分解型多機能マイクロゲルロボット」の実現を目的とする.
    今年度は,まず提案するロボットの加工と駆動,及び合体・分解昨日の原理確認を行った.
    提案するロボットを作製するために,温度応答性ゲルであるPNIPAAmをマイクロゲルアクチュエータとして用い,そこにシクロデキストリンとアゾベンゼンを混合し,フォトリソグラフィーによりマイクロパターンを作製できることを確認した.また,この材料を用いてロボットを作製し,そこにレーザーパターン光照射系を用いて光を照射することにより,ゲルアクチュエータを部分的に駆動し,ロボットの移動を行うことに成功した.移動に適したロボットのデザインを数パターン作製し,それぞれのロボットで移動と方向転換ができるかどうか,を評価した
    さらに,作製したロボットに可視光と紫外線を照射することで,ロボット同士の付着力が変わることを確認した.この評価には,傾けた基板を用意し,その上をロボットが滑走する速度を評価することで,付着力(摩擦力)の変化を評価した.この評価から,提案している合体・分解型のマイクロロボットの原理確認を行うことに成功した.
    今後は,付着力の定量評価を行うとともに,様々なデザインのロボットを作製して,駆動と合体・分解に適した設計を決定する予定である.

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  • Mass production of cell spheroid based on a vibration-induced flow

    Grant number:19H02115  2019.4 - 2022.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)  Chuo University

    Hayakawa Takeshi

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    Authorship:Principal investigator 

    Grant amount: \17810000 ( Direct Cost: \13700000 、 Indirect Cost: \4110000 )

    In this research, we proposed mass production method of sell spheroids based on a vibration-induced flow. Vibration-induced flow is local flow induced around vibrating microstructure. Thus, by patterning micropillars in ring arrangement on a chip and applying vibrations to the chip, vortex flow is induced at the center of the structures. By utilizing this vortex flow, we succeeded in forming cell spheroids. Furthermore, by patterning array of the structure, we succeeded in fabricating a few hundred cell spheroids. Next, we developed shape evaluation system of cell spheroids. We also used vibration-induced flow to evaluate 3D shape of cell spheroids. By rotating cell spheroids and observing with microscope, we can evaluate 3D shape of cell spheroids. We succeeded in developing rotation method of microobjects such as cell spheroids and demonstration of feedback control of orientation of microparticles. We will construct 3D shape information of cell spheroids with this system.

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  • ソフト&ウェット高分子材料への三次元配線技術

    2021 - 2022

    小笠原敏晶記念財団  一般研究助成  中央大学

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    Authorship:Principal investigator 

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  • 振動誘起流れを用いたナノバイオ物質の分離・濃縮技術

    2021    

    科学技術振興機構  産学が連携した研究開発成果の展開 研究成果展開事業 研究成果最適展開支援プログラム(A-STEP) トライアウト トライアウトタイプ(標準)  中央大学

    早川 健

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    Authorship:Principal investigator 

    申請者は,マイクロ構造体に振動を印加することで構造体の周りに流れが発生する振動誘起流れという現象を用いた微細操作技術を提案してきた.本研究では,この技術を応用してウイルスやDNA,RNAなどのナノバイオ物質を分離・濃縮する技術を実現する.本技術の応用先である新興感染症研究や衛生検査等において,従来は生体内に微量に含まれるウイルスや細菌のDNA,RNAを増幅して解析していたが,本研究によりこれらを分離・濃縮する技術が実現されれば,前工程を省略し検査時間を大幅に短縮することが可能となる.本研究ではナノバイオ物質を濃縮可能なマイクロデバイスの開発を行い,従来技術の100倍以上の濃縮性能を達成する.

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    J-GLOBAL

  • マイクロ共振構造体を用いた微小流体制御技術の開発

    2019 - 2020

    三豊科学技術振興協会  研究助成  中央大学

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    Authorship:Principal investigator 

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  • 三次元・透明ソフトアクチュエータの作製

    2019 - 2020

    服部報公会  工学研究奨励援助金  中央大学

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    Authorship:Principal investigator 

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  • 振動誘起流れによる微小物体の超精密操作を目指した力学モデル構築

    2017.4 - 2019.3

    NSKメカトロニクス技術高度化財団  民間助成金 

    早川 健

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \2000000

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  • 振動誘起流れ現象の三次元流れ解析および三次元細胞操作への応用

    2017.4 - 2019.3

    文部科学省  科学研究費補助金(日本学術振興会・文部科学省)-若手研究(B) 

    早川 健

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \4290000

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  • セレンディピターの流体制御システムの開発

    2017.4 - 2019.3

    JST ImPACT  受託研究費 

    合田圭介

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    Grant type:Competitive

    Grant amount: \3500000

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  • 振動誘起局所流れを用いた詰まることのない細胞フィルター

    2018 - 2019

    公益社団法人 日揮・実吉奨学会  研究助成  中央大学

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  • 振動誘起流れの周波数変調を用いたオープンチップでの細胞フィルター,トラップ操作

    2015.4 - 2017.3

    文部科学省  科学研究費補助金(日本学術振興会・文部科学省)-若手研究(スタートアップ) 

    早川 健

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \2990000

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  • 超高速光駆動によるマイクロツールの細胞内挿入及び単一細胞温度計測

    2013.4 - 2015.3

    文部科学省  文部科学省科学研究費補助金,学振特別研究員(DC2) 

    早川 健

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \2000000

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Intellectual property rights

  • 誘電エラストマアクチュエータの製造方法、及び、誘電エラストマアクチュエータ

    小池 夏実,中野 駿

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    Application no:特願2020-169884  Date applied:2020.10.7

    Announcement no:特開2022-061748  Date announced:2022.4.19

    Applicant (Organization):学校法人中央大学

  • 単一粒子分離デバイス、単一粒子分離キット及び単一粒子移送方法

    佐藤 康史

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    Application no:特願2019-014358  Date applied:2019.1.30

    Announcement no:特開2020-120608  Date announced:2020.8.13

    Applicant (Organization):学校法人中央大学

  • 液体混合装置及び液体混合方法

    鈴木 宏明,松井 駿幸,金子 完治

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    Application no:特願2018-203242  Date applied:2018.10.29

    Announcement no:特開2020-069411  Date announced:2020.5.7

    Registration no:特許第7178012号  Date registered:2022.11.16 

    Applicant (Organization):学校法人中央大学

  • 粒子分離用装置及び粒子分離方法

    北田 尚暉

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    Application no:特願2018-106401  Date applied:2018.6.1

    Announcement no:特開2019-208418  Date announced:2019.12.12

    Registration no:特許第7311870号  Date registered:2023.7.11 

    Applicant (Organization):学校法人中央大学

Allotted class

  • 2023   力学Ⅰ   Department

  • 2023   力学Ⅱ   Department

  • 2023   力学演習   Department

  • 2023   卒業研究Ⅰ   Department

  • 2023   卒業研究Ⅱ   Department

  • 2023   機械力学Ⅱ   Department

  • 2023   精密機械工学のための数学Ⅰ   Department

  • 2023   精密機械工学のための数学Ⅱ   Department

  • 2023   精密機械工学プロジェクト   Department

  • 2023   精密機械工学概論   Department

  • 2023   マイクロマニピュレーション特論   Graduate school

  • 2023   マイクロ・ナノロボティクス特論   Graduate school

  • 2023   精密工学特殊論文研修Ⅰ   Graduate school

  • 2023   精密工学特殊論文研修Ⅱ   Graduate school

  • 2023   精密工学特殊論文研修Ⅲ   Graduate school

  • 2023   精密工学特殊論文研修Ⅳ   Graduate school

  • 2023   精密工学特殊論文研修Ⅴ   Graduate school

  • 2023   精密工学特殊論文研修Ⅵ   Graduate school

  • 2023   精密工学特論   Graduate school

  • 2023   精密工学論文研修第一   Graduate school

  • 2023   精密工学論文研修第三   Graduate school

  • 2023   精密工学論文研修第二   Graduate school

  • 2023   精密工学論文研修第四   Graduate school

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Committee Memberships

  • 2023.1 - 2024.12

    計測自動制御学会   代議員  

  • 2023.4 - 2024.3

    Treasurer  

  • 2023.4 - 2024.3

    日本機械学会   東京ブロック 第30期(2023年度) 商議員  

  • 2022.4 - 2023.3

    Treasurer  

  • 2022.4 - 2023.3

    日本機械学会   東京ブロック 第29期(2022年度) 商議員  

  • 2020.12 - 2022.10

    日本ロボット学会   研究奨励賞選考委員会委員  

  • 2020.12 - 2022.10

    日本ロボット学会   学術講演会関連賞選考小委員  

  • 2022.1 - 2022.5

    化学とマイクロ・ナノシステム学会   第45回研究会 実行委員  

  • 2021.4 - 2022.3

    Treasurer  

  • 2021.1 - 2021.5

    化学とマイクロ・ナノシステム学会   第43回研究会 実行委員  

  • 2020.4 - 2021.3

    Treasurer  

  • 2020.7 - 2020.12

    化学とマイクロ・ナノシステム学会   第42回研究会 実行委員  

  • 2020.1 - 2020.12

    計測自動制御学会 システムインテグレーション部門   ノミネーション委員  

  • 2020.1 - 2020.12

    計測自動制御学会 システムインテグレーション部門   特命会計アドバイザ  

  • 2019.4 - 2020.3

    Treasurer  

  • 2018.4 - 2020.3

    精密工学会   学術交流委員会  

  • 2019.1 - 2019.12

    計測自動制御学会 システムインテグレーション部門   会計幹事  

  • 2019.1 - 2019.12

    Treasurer  

  • 2018.12 - 2019.7

    計測自動制御学会   SICE60周年に向けた未来社会・産業・SICEの将来像検討チーム  

  • 2018.4 - 2019.3

    Treasurer  

  • 2018.1 - 2018.12

    計測自動制御学会 システムインテグレーション部門   会計副幹事  

  • 2017.4 - 2018.3

    精密工学会 2018年度春季大会   運営委員  

  • 2017.4 - 2017.12

    Treasurer  

  • 2017.1 - 2017.12

    計測自動制御学会 システムインテグレーション部門   会計幹事補  

  • 2017.9 -  

    日本電気学会   マイクロ・ナノバイオ医療デバイス調査専門委員会  

  • 2016.4 - 2016.12

    Treasurer  

  • 2015.4 - 2015.12

    第16回計測自動制御学会システムインテグレーション部門講演会(SI2015)   現地担当委員  

  • 2015.4 - 2015.12

    26th 2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS2015)   Treasurer  

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