Updated on 2024/02/01

写真a

 
AZAI Chihiro
 
Organization
Faculty of Science and Engineering Associate Professor
Other responsible organization
Biological Sciences Course of Graduate School of Science and Engineering, Master's Program
Biological Sciences Course of Graduate School of Science and Engineering, Doctoral Program
Contact information
The inquiry by e-mail is 《here
External link

Degree

  • 博士(理学) ( 大阪大学 )

  • 修士(理学) ( 大阪大学 )

Education

  • 2010.6
     

    Osaka University   doctor course   completed

  • 2007.3
     

    Osaka University   master course   completed

  • 2005.3
     

    Kobe University   graduated

Research History

  • 2023.4 - Now

    Chuo University   Department of Biological Sciences, Faculty of Science and Engineering   Associate Professor

  • 2018.4 - 2023.3

    Ritsumeikan University   Department of Bioinformatics, College of Llife Sciences   Lecturer (Assistant Professor)

  • 2013.4 - 2018.3

    Ritsumeikan University   Department of Bioinformatics, College of Llife Sciences   Assistant Professor

  • 2011.4 - 2013.3

    Nagoya University   Graduate School of Science   Research Fellow (PD) of the Japan Society for the Promotion of Science

  • 2009.4 - 2011.3

    Osaka University   Graduate School of Science   Research Fellow (DC) of the Japan Society for the Promotion of Science

Professional Memberships

  • International Society of Photosynthesis Research

  • The Japanese Society of Plant Physiologists

  • The Japanese Society of Photosynthesis Research

  • The Biophysical Society of Japan

Research Interests

  • Photosynthesis, Electron transfer, Molecular evolution, Photosynthetic bacteria

Research Areas

  • Life Science / Genome biology

  • Life Science / Structural biochemistry

  • Life Science / Molecular biology

  • Life Science / Biophysics

  • Life Science / Functional biochemistry

  • Life Science / Evolutionary biology

  • Life Science / Plant molecular biology and physiology

  • Life Science / Applied microbiology

  • Life Science / Applied biochemistry

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Papers

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Books

MISC

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Presentations

  • Electrostatic Binding of Water-soluble Subunits on the Photosynthetic Reaction Center of Green Sulfur Bacteria

    Tomomi Inagaki, Kazuki Terauchi, Chihiro Azai

    The 64th Annual Meeting of the Japanese Society of Plant Physiologists 

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    Event date: 2023.3    

    Language:Japanese   Presentation type:Oral presentation (general)   Country:Japan  

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  • Sulfur-Dependent Photoinhibition in Anaerobic Culture of Green Sulfur Bacteria

    Masahiko Higashiguchi, Kazuki Terauchi, Chihiro Azai

    The 64th Annual Meeting of the Japanese Society of Plant Physiologists  2023.3 

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    Event date: 2023.3    

    Language:Japanese   Presentation type:Oral presentation (general)   Country:Japan  

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  • Light harvesting mechanism of the smallest type-I reaction center of green sulfur bacteria: mutational modifications and theoretical analysis

    Chihiro Azai, Hirozo Oh-oka, Shigeru Itoh, Hirotaka Kito, Akihiro Kimura

    The 64th Annual Meeting of the Japanese Society of Plant Physiologists  2023.3 

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    Event date: 2023.3    

    Language:Japanese   Presentation type:Oral presentation (general)   Country:Japan  

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  • 原核生物のゲノム情報から紐解く光合成の起源と進化 Invited

    西原 亜理沙, 塚谷 祐介, 浅井 智広, 延 優

    第2回原核光合成生物シンポジウム  ( 東北大学・川内キャンパス(ハイブリッド) )   2023.3 

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    Event date: 2023.3    

    Language:Japanese   Presentation type:Oral presentation (invited, special)   Country:Japan  

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  • 緑色硫黄細菌で見つかった嫌気的な光エネルギー散逸過程 Invited

    浅井 智広

    藍藻の分子生物学2022  ( かずさアカデミアホール )   2022.12 

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    Event date: 2022.12    

    Language:Japanese   Presentation type:Oral presentation (invited, special)   Country:Japan  

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  • Revisiting the function of special pair chlorophylls in type-1 reaction centers Invited International conference

    Chihiro Azai

    The International Symposium on Photosynthesis and Chloroplast Regulation  ( Kobe Fashion Mart, Kobe )   2022.11  The Grant-in-Aid for Scientific Research on Innovative Areas, New Photosynthesis

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    Event date: 2022.11    

    Language:English   Presentation type:Oral presentation (invited, special)   Country:Japan  

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  • 昨日の帰納はキノンの機能:タイプ1光合成反応中心にキノンは要るのか? Invited

    浅井智広

    第二回光合成タンパク質勉強会  ( オンライン )   2022.10 

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    Event date: 2022.10    

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)   Country:Japan  

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  • 緑色硫黄細菌の光合成反応中心複合体での三重項カロテノイドの生成

    浅井 智広, 木田 雅俊, 稲垣 知実, 小澄 大輔

    第34回カロテノイド研究談話会  ( 関西学院大学・西宮上ヶ原キャンパス(ハイブリッド) )   2022.9  日本カロテノイド研究会

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    Event date: 2022.9    

    Language:Japanese   Presentation type:Oral presentation (general)   Country:Japan  

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  • Charge separation induced by a lower-energy bacteriochlorophyll in the reaction center complex of Heliobacterium modesticaldum International conference

    Chihiro Azai, Risa Kojima, Kazuki Hinago, Masatoshi Kida, Tetsuya Yamamoto, Hirozo Oh-oka, Daisuke Kosumi, Yutaka Nagasawa

    The 17th International Symposium on Phototrophic Prokaryotes (ISPP2022)  ( ACC Liverpool, Liverpool )   2022.8 

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    Event date: 2022.8    

    Language:English   Presentation type:Oral presentation (general)   Country:United Kingdom  

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  • 緑色硫黄細菌Ⅰ型反応中心の励起状態の理論計算と主要BChl-a除去実験

    伊藤 繁, 木村 明洋, 鬼頭 宏任, 浅井 智広, 大岡 宏造

    第12回日本光合成学会年会  ( オンライン )   2022.5  日本光合成学会

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    Event date: 2022.5    

    Language:Japanese   Presentation type:Oral presentation (general)   Country:Japan  

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  • 緑色硫黄細菌の光合成反応中心複合体の穏和な解体

    稲垣 知実, 寺内 一姫, 浅井 智広

    第12回日本光合成学会年会  ( オンライン )   2022.5  日本光合成学会

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    Event date: 2022.5    

    Language:Japanese   Presentation type:Oral presentation (general)  

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  • 緑色硫黄細菌とメタン生成菌の人工共生系の構築 Invited

    浅井 智広

    新学術領域研究「革新的光物質変換」最終公開シンポジウム  ( 淡路夢舞台国際会議場(ハイブリッド) )   2022.3  新学術領域研究「革新的光物質変換」総括班

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    Event date: 2022.3    

    Language:Japanese   Presentation type:Oral presentation (general)   Country:Japan  

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  • Photosynthetic hydrogen production in green sulfur bacteria

    3rd International Solar Fuels Conference & International Conference on Artificial Photosynthesis-2019  2019.11 

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  • Time-Resolved Spectroscopy of Electron Transfer Processes upon Selective Excitation in the Reaction Center Complex from Heliobacterium modesticaldum

    3rd International Solar Fuels Conference & International Conference on Artificial Photosynthesis-2019  2019.11 

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  • Energy Transfer Pathways from Carotenoids in The Photosynthetic Reaction Center Complex of Green Sulfur Bacteria

    3rd International Solar Fuels Conference & International Conference on Artificial Photosynthesis-2019  2019.11 

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  • Femtosecond Time-Resolved Transient Absorption Spectroscopy of Reaction Center of Green Sulfur Bacteria: Elucidation of Primary Dynamics of Photosynthesis

    3rd International Solar Fuels Conference & International Conference on Artificial Photosynthesis-2019  2019.11 

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  • Functional implication of carotenoid glucosides in the anaerobic photosynthesis of green sulfur bacteria

    Young meeting of 3rd International Solar Fuels Conference  2019.11 

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  • Anaerobic energy dissipation by carotenoid glycoside in photosynthetic green sulfur bacteria

    1st US-Japan Binational Meeting  2019.10 

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  • Improved preparation of the reaction center complex from the green sulfur bacterium Chlorobaculum tepidum

    The 57th Annual Meeting of the Biophysical Society of Japan  2019.9 

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  • Carotenoid glycoside quenches bacteriochlorophyll a fluorescence in the photosynthetic reaction center complex of green sulfur bacteria

    The 57th Annual Meeting of the Biophysical Society of Japan  2019.9 

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  • Isolation of the Rieske/cytochrome b complex from green sulfur bacteria and interaction of the Rieske protein with cytochrome c-556

    The 57th Annual Meeting of the Biophysical Society of Japan  2019.9 

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  • 緑色硫黄細菌の光合成反応中心に結合したカロテノイドのアンテナ機能

    第33回カロテノイド研究談話会  2019.9 

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  • Functional Consequence of Glycosyl Transfer to Carotenoid in Photosynthesis of Green Sulfur Bacteria

    15th International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments (SNCPP19)  2019.6 

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  • 緑⾊硫⻩細菌のRieske/cytb複合体の精製検討とcyt c-556との相互作⽤部位の解析

    第10回 日本光合成学会年会  2019.5 

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  • 緑⾊硫⻩細菌から均⼀な反応中⼼複合体標品を得るための⼯夫

    第10回 日本光合成学会年会  2019.5 

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  • 緑⾊硫⻩細菌反応中⼼の光合成初期過程ダイナミクス

    第10回 日本光合成学会年会  2019.5 

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  • 酸素によるシアノバクテリア時計タンパク質KaiCのATPase活性阻害

    第10回 日本光合成学会年会  2019.5 

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  • 光応答性CheAタンパク質の創⽣ 〜異なる2つのクラスのヒスチジンキナーゼのキメラ化〜

    第10回 日本光合成学会年会  2019.5 

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  • カロテノイド配糖体エステルの緑⾊硫⻩細菌における光合成機能

    第10回 日本光合成学会年会  2019.5 

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  • 合成生物学時代のタンパク質の“ファイン”アート:ドメイン構造を超えたヒスチジンキナーゼのキメラ化

    圧力が拓く生命科学の新領域「圧力生命科学」研究成果報告会 〜あっと驚くタンパク質研究〜  2019.3 

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  • Primary dynamics of photosynthetic process in the reaction center of green sulfur bacteria

    The Chemical Society of Japan  2019.3 

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  • Defect of photosynthetic activity in the cruC gene mutant of the green sulfur bacterium Chlorobaculum tepidum, which lost the ability to synthesize carotenoid glucoside ester

    第60回 日本植物生理学会年会  2019.3 

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  • Interaction analysis between the Rieske/cyt b complex and c-type cytochromes in green sulfur bacteria

    第60回 日本植物生理学会年会  2019.3 

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  • Enhancement of charge separation in the photosynthetic reaction center of the green sulfur bacterium Chlorobaculum tepidum with external phylloquinones

    第60回 日本植物生理学会年会  2019.3 

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  • Creation of blue-light-responsive CheA by fusion of two different-class histidine kinases

    第60回 日本植物生理学会年会  2019.3 

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  • Circadian oscillation of cyanobacterial clock protein KaiC under anaerobic conditions

    第60回 日本植物生理学会年会  2019.3 

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  • 緑色硫黄光合成細菌におけるシアノバクテリア生物時計の細胞内再構成

    第13回日本ゲノム微生物学会年会  2019.3 

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  • 概日振動発振における時計タンパク質KaiCのC末端領域の機能解析

    第13回日本ゲノム微生物学会年会  2019.3 

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  • [FeFe]型ヒドロゲナーゼHydA1 の緑⾊硫⻩細菌Chlorobaculum tepidum細胞内での成熟化に対する鉄イオン添加の促進効果

    新学術領域研究「⾰新的光物質変換」第2回公開シンポジウム  2019.1 

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  • 緑⾊硫⻩細菌の光合成反応中⼼コアアンテナ系におけるカロテノイド配糖体エステルによる超⾼速消光

    新学術領域研究「⾰新的光物質変換」第2回公開シンポジウム  2019.1 

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  • Solubilization and purification of the Rieske/cytochrome b complex in green sulfur bacteria

    The 56th Annual Meeting of the Biophysical Society of Japan  2018.9 

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  • The orientation of menaquinone in the heliobacterial reaction center analyzed with the EPR spectroscopy

    The 56th Annual Meeting of the Biophysical Society of Japan  2018.9 

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  • Symmetry or asymmetry? - Site-specific structural modification of the homodimeric photosynthetic reaction center of green sulfur bacteria

    The 56th Annual Meeting of the Biophysical Society of Japan  2018.9 

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  • X-ray structure of the type-I reaction center from Heliobacterium modesticaldum at 3.2 Å resolution

    The 56th Annual Meeting of the Biophysical Society of Japan  2018.9 

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  • 緑色硫黄細菌Chlorobaculum tepidumのカロテノイド配糖体エステル欠損の光合成活性への影響

    第32回カロテノイド研究談話会  2018.9 

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  • Fluorescence lifetime analysis of the green sulfur bacterium Chlorobium tepidum mutant devoid of a reaction center specific carotenoid glycoside

    ISPR Conference on Microbial Photosynthesis  2018.8 

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  • Glycolipid and chlorophyll a biosyntheses are required for the heterologous assembly of the type-I photochemical reaction center in purple bacteria

    16th International Symposium on Phototrophic Prokaryotes  2018.8 

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  • Reconstitution of circadian oscillation of KaiC from the nonheterocystous nitrogen-fixing cyanobacterium Leptolyngbya boryana

    16th International Symposium on Phototrophic Prokaryotes  2018.8 

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  • BciA works as an auxiliary but highly active divinyl reductase in the purple photosynthetic bacterium Rhodobacter capsulatus

    16th International Symposium on Phototrophic Prokaryotes  2018.8 

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  • Menaquinone (MQ) in the reaction center of Heliobacterium modesticaldum studied by ESP-EPR signal of P800+MQ–: its implication in the 3D structure

    16th International Symposium on Phototrophic Prokaryotes  2018.8 

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  • Creation of phototactic CheA - Chimerization of two different classes of histidine kinases -

    14th International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments (SNCPP18)  2018.6 

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  • 緑色硫黄細菌のRieske/cyt b型シトクロム複合体の可溶化・精製の試み

    第9回 日本光合成学会年会  2018.5 

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  • 紅色光合成細菌Rhodobactor capsulatusのBciAによる迅速なクロロフィリドaのC8ビニル基還元

    第9回 日本光合成学会年会  2018.5 

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  • シアノバクテリアの時計タンパク質KaiCにおけるCII六量体リングのリン酸化と構造変化

    第59回 日本植物生理学会年会  2018.3 

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  • 異なるクラスのヒスチジンキナーゼを融合した人工青色光受容体タンパク質の作製

    第59回 日本植物生理学会年会  2018.3 

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  • 光化学系I型反応中心の紅色光合成細菌への導入

    第59回 日本植物生理学会年会  2018.3 

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  • 軟X線顕微鏡を用いた糸状性シアノバクテリアにおける光合成発生酸素の直接観察

    第59回 日本植物生理学会年会  2018.3 

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  • 光応答性CheA タンパク質の設計:異なるクラスのヒスチジンキナーゼのキメラ化

    第12回日本ゲノム微生物学会年会  2018.3 

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  • Analyses of electron transfer reactions on donor and acceptor sides in heliobacterial photosynthetic reaction center

    The 55th Annual Meeting of the Biophysical Society of Japan  2017.9 

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  • Visualization of intracellular element concentration in cyanobacteria with soft x-ray live cell imaging

    The 55th Annual Meeting of the Biophysical Society of Japan  2017.9 

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  • Conformational change by phosphorylation and ATP hydrolysis in the cyanobacterial circadian oscillator KaiC

    The 55th Annual Meeting of the Biophysical Society of Japan  2017.9 

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  • 緑色硫黄細菌Chlorobaculum tepidumの異種遺伝子発現系による、緑藻由来[FeFe]型ヒドロゲナーゼの高発現と水素生産

    第8回 日本光合成学会年会  2017.5 

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  • ヘリオバクテリア反応中心コアタンパクとFd1/Fd2の再構成実験

    第8回 日本光合成学会年会  2017.5 

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  • 分子状酸素の直接観察を目指したシアノバクテリア細胞内の元素分布の軟X線顕微鏡イメージング

    第8回 日本光合成学会年会  2017.5 

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  • 緑⾊硫⻩光合成細菌におけるシアノバクテリア時計タンパク質の強制発現

    第8回 日本光合成学会年会  2017.5 

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  • 環境の酸素レベルによるシアノバクテリア⽣物時計再構成系の周期⻑変化

    第8回 日本光合成学会年会  2017.5 

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  • シアノバクテリア時計タンパク質KaiC のCIドメインのATPase 活性に依存した2状態変換

    第58回 日本植物生理学会年会  2017.3 

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  • 軟X線顕微鏡による糸状性シアノバクテリアAnabaena sp. PCC 7120内の窒素分布の可視化

    第58回 日本植物生理学会年会  2017.3 

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  • 絶対嫌気性の光合成細菌Chlorobaculum tepidumの異種遺伝子発現系を利用した緑藻[FeFe]型ヒドロゲナーゼの細胞内成熟化

    第58回 日本植物生理学会年会  2017.3 

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  • Reconstitution experiment of the PshB protein with the photosynthetic reaction center core protein of Heliobacteriaum modesticaldum

    第58回 日本植物生理学会年会  2017.3 

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  • N-terminal nucleotide sequence dependency of anaerobic protein overexpression in the photosynthetic green sulfur bacterium Chlorobaculum tepidum

    2017 International Conference on Artificial Photosynthesis  2017.3 

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  • 窒素固定性シアノバクテリアLeptolyngbya boryanaにおける時計タンパク質KaiCの生化学的解析

    第11回 ゲノム微生物学会年会  2017.3 

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  • 光合成反応中心複合体に潜む構造機能相関の実験的探索

    第9回 BKCバイオインフォマティクス研究会  2017.2 

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  • 透過型軟X線顕微鏡によるシアノバクテリアの元素選択的な細胞内構造の観察

    PF研究会「PF挿入光源ビームラインBL-19の戦略的利用に関する研究会」  2017.1 

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  • Analyses of transient absorption changes and low-temperature fluorescence in the photosynthetic reaction center of heliobacteria

    第54回 日本生物物理学会  2016.11 

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  • Conversion between two hexameric states of KaiC for cyanobacterial circadian oscillation

    IGER International Symposium on Biological Rhythms  2016.11 

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  • Circadian Clock in the Nonheterocystous Nitrogen Fixing Cyanobacterium Leptolyngbya boryana

    4th Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation  2016.10 

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  • シアノバクテリア時計タンパク質KaiCにおけるATP加水分解に伴う構造変換

    生物資源研究センター第1回シンポジウム  2016.9 

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  • Determination of Carbon-to-Nitrogen Ratio in the Filamentous and Heterocystous Cyanobacterium Anabaena sp. PCC 7120 with Single-cell Soft X-ray Imaging

    13th International Conference on X-ray Microscopy  2016.8 

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  • Functions of Evolutionally Conserved Antenna Chlorophylls in Type-1 Photosynthetic Reaction Centers

    Photosynthetic and Respiratory Complexes: From Structure to Function  2016.8 

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  • Mutagenic analysis of conserved antenna chlorophylls in type-1 photosynthetic reaction centers

    The 17th International Congress on Photosynthesis Research  2016.8 

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  • Orientations of FA/FB and temperature dependencies of their signal intensities in the homodimeric type 1 reaction center of Heliobacterium modesticaldum

    The 17th International Congress on Photosynthesis Research  2016.8 

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  • Structural homology around the special pair chlorophylls among type-1 reaction centers

    Satellite Meeting on Photosynthesis  2016.7 

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  • Influence of codon bias on the anaerobic protein overproduction in the phototrophic green sulfur bacterium Chlorobaculum tepidum

    Satellite Meeting on Photosynthesis  2016.7 

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  • The conformational change and protomer exchange of KaiC hexamer in the cyanobacterial circadian clock

    The 16th Annual Meeting of the Protein Science Society of Japan  2016.6 

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  • ヘリオバクテリア光合成反応中心のエネルギー移動経路に関する解析

    第7回 日本光合成学会年会  2016.5 

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  • 緑色硫黄細菌での異種遺伝子発現におけるコドン使用頻度の影響

    第7回 日本光合成学会年会  2016.5 

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  • ホモダイマー型光合成反応中心の構造と機能:光合成の古くて新しいお話

    第16回光科学若手研究会  2016.4 

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  • ヘリオバクテリア光合成反応中心の低温蛍光解析

    第57回 日本植物生理学会年会  2016.3 

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  • ヘリオバクテリア由来の1 型光合成反応中心の活性測定

    第57回 日本植物生理学会年会  2016.3 

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  • 緑色硫黄細菌Chlorobaculum tepidumにおけるGFP 発現の塩基配列依存性

    第10回 日本ゲノム微生物学会年会  2016.3 

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  • シアノバクテリア生物時計再構成系における周期長決定機構の解析

    第10回 日本ゲノム微生物学会年会  2016.3 

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  • 緑藻型[FeFe]ヒドロゲナーゼとH クラスター生合成系の緑色硫黄光合成細菌における嫌気的共発現

    第4回公開シンポジウム 新学術領域研究「人工光合成による太陽光エネルギーの物質変換:実用化に向けての異分野融合」  2016.1 

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  • 軟X線顕微鏡による糸状性シアノバクテリアの炭素窒素比の決定

    第29回 日本放射光学会年会・放射光科学合同シンポジウム  2016.1 

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  • Stability and spectroscopic characterization of heliobacterial reaction center core protein incorporated into nanoporous silica particles

    The 53rd Annual Meeting of the Biophysical Society of Japan  2015.9 

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  • Direct observation of nitrogen fixation in filamentous cyanobacteria by using soft X-ray microscopy

    The 53rd Annual Meeting of the Biophysical Society of Japan  2015.9 

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  • Construction of artificial heterodimeric photosynthetic reaction center in the green sulfur bacterium Chlorobaculum tepidum

    15th International Symposium on Phototrophic Prokaryotes  2015.8 

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  • Bacteriochlorophyll a synthesis in the green sulfur bacterium Chlorobaculum tepidum mutant lacking BchX, a subunit of chlorophyllide a oxidoreductase

    15th International Symposium on Phototrophic Prokaryotes  2015.8 

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  • A Rieske/Cytochrome b Complex in the Green Sufur Bacterium Chlorobaculum tedium

    15th International Symposium on Phototrophic Prokaryotes  2015.8 

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  • Correlation between hexameric states and phosphorylation levels of the cyanobacterial circadian clock protein KaiC

    15th International Symposium on Phototrophic Prokaryotes  2015.8 

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  • Heliobacterial type-I homodimer reaction center uses menaquinone as the secondary electron acceptor, at a little different location from that of phylloquinone in PSI

    15th International Symposium on Phototrophic Prokaryotes  2015.8 

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  • The roles of two structural states of the KaiC hexamer in the cyanobacterial clock protein complex

    第15回 日本蛋白質科学会年会  2015.6 

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  • Characterization of C8-vinyl reduction activities of BciA from Rhodobacter capsulatus

    11th International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments (SNCPP15)  2015.5 

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  • Ion exchange chromatography and gel filtration chromatography analyses of cyanobacterial clock protein KaiA

    11th International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments (SNCPP15)  2015.5 

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  • Insight into the function of alanine-422 residue of KaiC involved in autonomous synchronization of circadian phosphorylation rhythm

    11th International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments (SNCPP15)  2015.5 

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  • Temperature compensation of KaiC phosphorylation in cyanobacterial circadian clock: Biochemical analysis of two homologous domains of KaiC

    11th International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments (SNCPP15)  2015.5 

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  • ホモダイマー型光合成反応中心の分子生物学的な構造機能解析

    第6回 日本光合成学会年会およびシンポジウム  2015.5 

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  • ホモダイマー光合成反応中心複合体の人工的ヘテロダイマー化

    第62回 日本生化学会近畿支部例会  2015.5 

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  • シアノバクテリア生物時計再構成系におけるKaiCの二状態変化とATPase活性

    第62回 日本生化学会近畿支部例会  2015.5 

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  • タイプ1 光合成反応中心の保存されたアンテナクロロフィルの役割

    第56回 日本植物生理学会年会  2015.3 

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  • A cytochrome bc complexe is Rieske/cytochrome b-type in photosynthetic green sulfur bacteria

    第56回 日本植物生理学会年会  2015.3 

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  • 光合成細菌Rhodobacter capsulatusにおけるジビニル還元酵素BciAのC8ビニル基還元活性解析

    第56回 日本植物生理学会年会  2015.3 

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  • 大腸菌におけるシアノバクテリア時計遺伝子の発現とその制御解析

    第9回 日本ゲノム微生物学会年会  2015.3 

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  • 緑色硫黄細菌におけるシアノバクテリア時計遺伝子の異種発現

    第9回 日本ゲノム微生物学会年会  2015.3 

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  • 糸状シアノバクテリアの元素選択的観察

    第28回 日本放射光学会年会  2015.1 

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  • Discrimination between Two Structural States of the KaiC Hexamer in the Cyanobacterial Clock Protein Complex

    The 3rd International Symposium "Dynamical ordering of biomolecular systems for creation of integrated functions"  2015.1 

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  • Isolation of Rieske/cytochrome b complex from a green sulfur bacterium Chlorobaculum tepidum

    The 52nd Annual Meeting of the Biophysical Society of Japan  2014.9 

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  • Two Disconnected Antenna Chlorophyll Pools in Type-1 Photosynthetic Reaction Center of Green Sulfur Bacteria

    The 52nd Annual Meeting of the Biophysical Society of Japan  2014.9 

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  • Structural studies of [FeFe] hydrogenase maturation from green alga Chlamydomonas reinhardtii

    The 52nd Annual Meeting of the Biophysical Society of Japan  2014.9 

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  • Effect of redox state of KaiA on the cyanobacterial circadian clock in vitro

    10th International Workshop on Supramolecular Nanoscience of Chemically Programmed Pigments (SNCPP14)  2014.5 

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  • ヘリオバクテリアI型反応中心のキノン反応・部位はPSIと異なる

    第5回 日本光合成学会年会  2014.5 

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  • シアノバクテリア生物時計再構成系におけるKaiCの周期的構造変化解析

    第5回 日本光合成学会年会  2014.5 

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  • 緑色硫黄細菌のタイプI型RC はPS Iとは異なるエネルギー移動系をもつ

    第5回 日本光合成学会年会  2014.5 

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  • 緑色硫黄細菌Chlorobaculum tepidumにおいてbchX遺伝子はバクテリオクロロフィルa生合成に必須ではない

    第55回 日本植物生理学会年会  2014.3 

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  • 多孔質ガラス中のナノ空間における光化学系IIコア複合体からcyt c6への電子伝達

    第55回 日本植物生理学会年会  2014.3 

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  • 多孔性シリカ粒子に吸着したヘリオバクテリア反応中心コアタンパクの分光学的諸性質

    第55回 日本植物生理学会年会  2014.3 

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  • Site-specific structural modification around the special pair of homodimeric photosynthetic reaction center in green sulfur bacteria

    The 16th International Congress on Photosynthesis Research  2013.8 

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  • Studies on the photosynthetic reaction centre from the green sulfur bacterium Chlorobaculum tepidum

    The 16th International Congress on Photosynthesis Research  2013.8 

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  • Heliobacterial Reaction Center Core Complex in Nanoporous Silica Material

    The 16th International Congress on Photosynthesis Research  2013.8 

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  • Inactivation of BchX, a subunit of chlorophyllide a oxidoreductase, in the green sulfur bacterium Chlorobaculum tepidum

    Light Harvesting Satellite Meeting 2013  2013.8 

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  • Studies on the photosynthetic reaction centre from the green sulphur bacterium Chlorobaculum tepidum

    Light Harvesting Satellite Meeting 2013  2013.8 

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  • 光合成反応中心複合体における非対称な電子移動

    CREST有機太陽電池シンポジウム  2013.7 

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  • 緑色硫黄細菌の一次電子供与体P840の酸化還元差スペクトルの帰属再検討

    第4回 日本光合成学会年会  2013.5 

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  • 部位特異的変異導入によるホモダイマ―型光合成反応中心の構造改変

    第54回 日本植物生理学会年会  2013.3 

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  • 光合成反応中心の構造機能相関の解明に向けた分子生物学的なアプローチ

    第1回 立命館大学BKC 生体分子ネットワークセミナー  2013.1 

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  • 緑色硫黄細菌のホモダイマ—光合成反応中心において部位特異的変異が一次電子供与体周辺の構造に与える影響

    第50回 日本生物物理学会年会  2012.9 

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  • Studies on the reduction processes from chlorophyll a estrified with geranylgeraniol to that with ∆2,6-phytadienol in the green sulfur bacterium Chlorobaculum tepidum

    14th International Symposium on Phototrophic Prokaryotes  2012.8 

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  • The gene expression system in the Green Sulfur Bacterium Chlorobaculum tepidum by Conjugative Plasmid Transfer

    14th International Symposium on Phototrophic Prokaryotes  2012.8 

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  • 緑色硫黄細菌の光合成反応中心の部位特異的変異体:一次電子供与体P840周辺の水素結合構造の改変を例として

    第3回 日本光合成学会大会  2012.6 

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  • 人工ヘテロダイマー光合成反応中心複合体の作製と精製

    第53回 日本植物生理学会年会  2012.3 

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  • The molecular biological method for the construction of artificial heterodimeric photosynthetic reaction center in green sulfur bacteria

    The 5th Asia and oceania Conference on Photobiology  2011.8 

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  • 絶対嫌気性の光合成細菌Chlorobaculum tepidumの外来遺伝子発現系

    第2回 日本光合成学会大会  2011.6 

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  • Reaction of A1-menaquinone in type I reaction center of Heliobacterium modesticaldum at cryogenic temperature

    15th International Congress of Photosynthesis (PS2010)  2010.8 

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  • The His-tagged reaction center complex of the green sulfur bacterium Chlorobaculum tepidum

    15th International Congress of Photosynthesis (PS2010)  2010.8 

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  • 人工的ヘテロダイマー化を可能にするホモダイマー型光合成反応中心への部位特異的変異導入法

    第18回 光合成の色素系と反応中心に関するセミナー  2010.7 

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  • コアタンパク質の偽二倍体化によるホモダイマー型光合成反応中心のヘテロダイマー化

    第1回 日本光合成学会大会  2010.6 

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  • ホモダイマー型光合成反応中心のアフィニティ精製と分光学的性質

    第47回 日本生物物理学会年会  2009.10 

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  • The role of cytochrome c-554 in the electron transfer pathways in the green sulfur bacterium Chlorobium tepidum

    13th International Symposium on Phototrophic Prokaryotes  2009.8 

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  • 緑色硫黄細菌Chlorobium tepidumの光合成反応中心複合体のアフィニティクロマトグラフィによる調製

    第50回 日本植物生理学会年会  2009.3 

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  • 緑色硫黄細菌Chlorobium tepidumのホモダイマー型光化学系反応中心への変異導入法の検討

    第49回 日本植物生理学会年会  2008.3 

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  • 緑色硫黄光合成細菌の電子伝達経路の分岐とその生理的意義

    第14回 日本光生物学協会年会  2007.7 

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  • Detection of quinone function in the homodimeric type-I reaction center of Heliobacterium modesticaldum

    14th International Congress on Photosynthesis (PS2007)  2007.7 

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  • Bifurcated electron donations from quinol oxidoreductase and soluble CycA to cytochrome cz of the photosynthetic reaction center complex in the green sulfur bacterium Chlorobium tepidum

    14th International Congress on Photosynthesis (PS2007)  2007.7 

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  • 緑色硫黄細菌における反応中心への競合的な電子供与反応

    第15回 光合成の色素系と反応中心に関するセミナー  2007.6 

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  • Fast electron donation from CycA to the homodimeric type-I reaction center of the green sulfur bacteria Chlorobium tepidum

    5th East Asian Biophysics Symposium & 44th Annual Meeting of the Biophysical Society of Japan  2006.11 

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  • Involvement of CycA in thiosulfate oxidatiton in the green sulfur bacteria Chlorobium tepidum

    12th International Symposium on Phototrophic Prokaryotes  2006.8 

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  • 緑色硫黄細菌Chlorobium tepidumの光合成電子伝達経路とリンクした硫黄酸化経路

    第43回 日本生物物理学会年会  2005.11 

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  • 紅藻オキツノリ着生藻の形態的、生理的特性

    第29回 日本藻類学会大会  2005.3 

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Awards

  • The 16th International Congress on Photosynthesis Research Poster Award

    2013.8   International Society of Photosynthesis Research  

  • 第3回日本光合成学会大会 優秀発表賞

    2012.6   日本光合成学会  

  • 第2回日本光合成学会大会 優秀発表賞

    2011.6   日本光合成学会  

Research Projects

  • 光合成における超硫黄分子の貢献

    Grant number:21H05266  2021.9 - 2026.3

    日本学術振興会  科学研究費助成事業  学術変革領域研究(A)  東北大学

    石丸 泰寛, 河野 優, 浅井 智広

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    Grant amount: \81900000 ( Direct Cost: \63000000 、 Indirect Cost: \18900000 )

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  • 超高速1分子スペクトル分光法で探る生体光反応の機能的ロバスト性

    Grant number:22H02030  2022.4 - 2025.3

    日本学術振興会  科学研究費助成事業  基盤研究(B)  東京工業大学

    近藤 徹, 浅井 智広

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    Grant amount: \17550000 ( Direct Cost: \13500000 、 Indirect Cost: \4050000 )

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  • Exploring photoprotective function of carotenoid under strictly anaerobic conditions

    Grant number:21K06220  2021.4 - 2024.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)  Ritsumeikan University

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    Grant amount: \4160000 ( Direct Cost: \3200000 、 Indirect Cost: \960000 )

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  • Development of photosynthetic methane production by hydrogen production in green sulfur bacteria

    Grant number:20H05119  2020.4 - 2022.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)  Ritsumeikan University

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    Grant amount: \6500000 ( Direct Cost: \5000000 、 Indirect Cost: \1500000 )

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  • Identification of pH-dependent electron transfer reactions in homodimeric reaction center complex

    Grant number:19H04734  2019.4 - 2021.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)  Ritsumeikan University

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    Grant amount: \5330000 ( Direct Cost: \4100000 、 Indirect Cost: \1230000 )

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  • Development of photosynthetic methane production by hydrogen production in green sulfur bacteria

    Grant number:18H05181  2018.4 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)  Ritsumeikan University

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    Grant amount: \5200000 ( Direct Cost: \4000000 、 Indirect Cost: \1200000 )

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  • Unveiling proton-motive force generation by bacterial type-1 photosynthetic reaction ceters

    Grant number:17H05731  2017.4 - 2019.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)  Ritsumeikan University

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    Grant amount: \6240000 ( Direct Cost: \4800000 、 Indirect Cost: \1440000 )

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  • Construction of artificial blue light receptor for synthetic phototaxis of purple photosynthetic bacteria

    Grant number:16K14907  2016.4 - 2018.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research  Ritsumeikan University

    Azai Chihiro, SHIMOJI Mamiko

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    Grant amount: \3640000 ( Direct Cost: \2800000 、 Indirect Cost: \840000 )

    Through the course of this project, we tried to construct a chimera of class I and II histidine kinases (HK) to construct a photoresponsive HK that enable us switch the rotation direction of bacterial flagellar motors by light irradiation. We designed an artificial blue-light receptor protein “YC”, which is a chimera of LOV-HK and CheA, based on homology in their atomic structures. Swarming assay of the YC-expressed E. coli showed acquired phototactic response by the light-dependent autophosphorylation activity of YC. The recombinant YC protein had a photoconvertable flavin cofactor that adducts to the conserved Cys residue of the LOV domain. This is the first successful report for functional fusion of the class I and II HKs.

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  • The mechanisms to control electron transfer reactions in the homodimeric photosynthetic reaction center based on its molecular structure

    Grant number:15K07026  2015.4 - 2018.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)  Osaka University

    Oh-oka Hirozo, Itoh Shigeru, Kurisu Genji, Azai Chihiro

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    Grant amount: \5200000 ( Direct Cost: \4000000 、 Indirect Cost: \1200000 )

    Light-dependent charge separation in photosynthesis is driven by the reaction center (RC) membrane protein complex that is classified into four types; the green bacterial or heliobacterial, the purple bacterial, the photosystem (PS) I and II RCs. Compared to the latter three types of RCs, the molecular architecture of heliobacterial RC is still poorly understood, in part due to the lack of high-resolution structural information. Structure of the RC from Heliobacterium modesticaldum has been solved to a resolution of 3.2 angstrom. Although the X-ray structure, which was reported by Arizona group in 2017, contained no quinone molecule within its, our crystal structure has indicated that it was contained and seemed to function as a secondary acceptor A1. The ESP-EPR signals, which was derived from the charge separated state of P800+A1-, suggested that the quinone molecule had an almost similar orientation to but a little bit different location from that in PS I.

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  • 高い水素発生活性を実現する[FeFe]型ヒドロゲナーゼの構造デザイン

    Grant number:15H00886  2015.4 - 2017.3

    日本学術振興会  科学研究費助成事業  新学術領域研究(研究領域提案型)  立命館大学

    浅井 智広

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    Grant amount: \3120000 ( Direct Cost: \2400000 、 Indirect Cost: \720000 )

    緑藻Chlamydomonas reinhardtiiの[FeFe]ヒドロゲナーゼHydA1 は、コンパクトで有りながら非常に高い水素発生活性をもつ酵素として知られているが、活性中心であるHクラスターの形成と保持に厳密に嫌気的な環境を必要とし、生化学的な解析や応用研究が困難である。本研究課題では、酸素分圧1 ppm 以下の嫌気環境でのタンパク質生産を容易に実現できる、絶対嫌気性の光合成細菌Chlorobaculum tepidumの外来遺伝子発現系を利用し、高い水素発生活性を保持したHydA1の大量生産系を確立することを主な目的としていた。
    28年度までの研究により、C. tepidumにおいて高レベルのタンパク質発現は遺伝子のN末端領域の塩基配列に強く依存することを明らかにした。29年度はこの研究成果を利用し、Hクラスター形成に必要な成熟化タンパク質であるHydE、HydF、HydGのHydA1との高レベル共発現を試みた。全長にわたるコドン使用頻度の最適化と、N末端に付加するアフィニティータグの塩基配列を検討した結果、Strepタグ付きの遺伝子の導入によってC. tepidum内でHydEFGを高発現できることがわかった。HydA1と共発現させた結果、ほぼ全てのHydA1にHクラスターを保持させることに成功した。
    驚くべきことに、HydA1とHydEFGのC. tepidum共発現株は、培養中に光依存的に水素ガスを発生させていることがわかった。HydA1は本来、光合成伝達系で働くフェレドキシンPetFをプロトン還元の電子供与体としているが、その分子認識は厳密ではなく、C. tepidumの細胞内では別の電子供与体が機能している可能性が示唆された。

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  • Functional analysis of quinone in the photosynthetic reaction center complex of green sulfur bacteria

    Grant number:25891025  2013.8 - 2015.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Research Activity Start-up  Ritsumeikan University

    AZAI Chihiro

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    Grant amount: \2730000 ( Direct Cost: \2100000 、 Indirect Cost: \630000 )

    A gene expression system by a broad host range plasmid was established in the green sulfur bacterium Chlorobaculum tepidum, which is a strictly anaerobic photosynthetic bacterium. Large-scale preparation of the homodimeric photosynthetic reaction center (RC) complex of green sulfur bacteria was easily achieved by over-expression of the His-tagged RC core-protein and its affinity purification.
    Electron transfer reactions in the green sulfur bacterial RC complex were observed by transient absorption spectroscopy from sub-nanosecond to millisecond time domains. The electron transfer pathway was affected by additives in the reaction solution; it was suggested that there is an unidentified cofactor serving as an alternate terminal electron acceptor of the normal iron-sulfur cluster in the green sulfur bacterial RC complex.

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Allotted class

  • 2023   卒業研究Ⅰ   Department

  • 2023   卒業研究Ⅱ   Department

  • 2023   応用生物学   Department

  • 2023   植物分子生理学   Department

  • 2023   生命科学英語上級1   Department

  • 2023   生命科学英語上級2   Department

  • 2023   生命科学英語初級   Department

  • 2023   生理・生化学実験   Department

  • 2023   生命科学特殊論文研修Ⅰ   Graduate school

  • 2023   生命科学特殊論文研修Ⅱ   Graduate school

  • 2023   生命科学特殊論文研修Ⅲ   Graduate school

  • 2023   生命科学特殊論文研修Ⅳ   Graduate school

  • 2023   生命科学特殊論文研修Ⅴ   Graduate school

  • 2023   生命科学特殊論文研修Ⅵ   Graduate school

  • 2023   生命科学特論   Graduate school

  • 2023   生命科学論文研修第一   Graduate school

  • 2023   生命科学論文研修第三   Graduate school

  • 2023   生命科学論文研修第二   Graduate school

  • 2023   生命科学論文研修第四   Graduate school

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Teaching Experience

  • English JP2

    Institution:Ritsumeikan University

  • 英語JP2

    Institution:立命館大学

  • Graduation Research 2

    Institution:Ritsumeikan University

  • 卒業研究2

    Institution:立命館大学

  • Experiments in Molecular Biology

    Institution:Ritsumeikan University

  • 分子生物学実験

    Institution:立命館大学

  • Experiments in Basic Biology

    Institution:Ritsumeikan University

  • 生物学基礎実験

    Institution:立命館大学

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