Updated on 2024/02/17

写真a

 
YOSHIDA Shotaro
 
Organization
Faculty of Science and Engineering Assistant Professor
Other responsible organization
Electrical, Electronic, and Communication Engineering Course of Graduate School of Science and Engineering, Master's Program
Contact information
The inquiry by e-mail is 《here
Profile

2011 Bachelor in Electrical and Information Engineering, The University of Tokyo, Japan. Major: Artificial Intelligence, Computer Science 2013 Master in Mechano-Informatics, The University of Tokyo, Japan. Major: BioMEMS, Neuroscience 2013-2016 JSPS research fellow (DC1) 2016 Ph.D in Mechano-Informatics, The University of Tokyo, Japan. Major: BioMEMS, Tissue engineering, Neuroscience 2016-2018 Post-doc researcher in Shoji Takeuchi lab, The University of Tokyo, Japan. Working on cell-sized liposomes and artificial cells. 2018-2020 Assistant Professor at Matsuhiko Nishizawa Lab, Tohoku University, Japan. Working on electrochemistry and bioelectronics. 2020-present Assistant Professor (PI), Department of Electrical, Electronic, and Communication Engineering, Faculty of Science and Engineering, Chuo University, Japan

External link

Degree

  • 博士(情報理工学) ( 東京大学 )

  • 修士(情報理工学) ( 東京大学 )

Education

  • 2016.3
     

    The University of Tokyo   Graduate School of Information Science and Technology   Mechano-Informatics (Doctoral Course)   doctor course   completed

  • 2013.3
     

    The University of Tokyo   Graduate School of Information Science and Technology   Mechano-Informatics (Master Course)   master course   completed

  • 2011.3
     

    The University of Tokyo   Faculty of Engineering   Information and Communication Engineering   graduated

  • 2009.3
     

    The University of Tokyo   College of Arts and Sciences   others

Research History

  • 2020.4 - Now

    Chuo University   Faculty of Science and Engineering Department of Electrical, Electronic, and Communication Engineering   Assistant Professor

  • 2018.4 - 2021.3

    IIS, The University of Tokyo

  • 2018.4 - 2020.3

    Tohoku University   Department of Finemechanics   Assistant Professor

  • 2016.4 - 2018.3

    The University of Tokyo   Institute of Industrial Science   Project researcher

  • 2013.4 - 2016.3

    日本学術振興会 特別研究員(DC1)

Professional Memberships

  • JAPANESE NEURAL NETWORK SOCIETY

  • THE JAPAN SOCIETY OF MECHANICAL ENGINEERS

  • THE INSTITUTE OF ELECTRICAL ENGINEERS OF JAPAN

  • 「細胞を創る」研究会

  • THE SOCIETY FOR CHEMISTRY AND MICRO-NANO SYSTEMS

Research Interests

  • マイクロ流体デバイス

  • Lab-on-a-chip

  • マイクロパターニング

  • MEMS

  • 神経科学

  • 情報科学

  • 有機エレクトロニクス

  • マイクロナノマシン

  • ソフトロボティクス

  • ソフトエレクトロニクス

  • バイオエレクトロニクス

  • μ-TAS

  • エネルギーデバイス

  • 皮膚科学

  • 再生医療

  • 組織工学

  • 電気化学

  • ウェアラブルデバイス

  • 神経回路

  • 人工知能

  • ブレインオンチップ

  • 人工細胞

  • バイオセンサ

Research Areas

  • Informatics / Intelligent informatics

  • Life Science / Basic brain sciences

  • Nanotechnology/Materials / Nanobioscience

  • Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Electron device and electronic equipment

  • Life Science / Neuroscience-general

  • Life Science / Biophysics

  • Informatics / Robotics and intelligent system

  • Informatics / Mechanics and mechatronics

  • Nanotechnology/Materials / Organic functional materials

  • Nanotechnology/Materials / Nano/micro-systems

  • Life Science / Biomedical engineering

  • Life Science / Biomaterials

  • Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Electric and electronic materials

  • Informatics / Intelligent robotics

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Papers

  • Enzyme electrode for glucose oxidation using low‐solubility 4‐aminodiphenylamine derivatives as electron mediator Reviewed

    Yosuke Masakari, Naoya Totsuka, Yasutomo Shinohara, Shotaro Yoshida, Hiroya Abe, Kotaro Ito, Matsuhiko Nishizawa

    Electrochemical Science Advances   2 ( 4 )   e2100036   2021.6

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1002/elsa.202100036

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/elsa.202100036

  • Porous Microneedle-Based Wearable Device for Monitoring of Transepidermal Potential Reviewed

    Yuina Abe, Ryohei Takizawa, Natsumi Kimura, Hajime Konno, Shotaro Yoshida, Matsuhiko Nishizawa

    Biomedical Engineering Advances   1   100004   2021.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.bea.2021.100004

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  • Transdermal electroosmotic flow generated by a porous microneedle array patch Reviewed

    Shinya Kusama, Kaito Sato, Yuuya Matsui, Natsumi Kimura, Hiroya Abe, Shotaro Yoshida, Matsuhiko Nishizawa

    Nature Communications   12 ( 1 )   658   2021.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    <title>Abstract</title>A microneedle array is an attractive option for a minimally invasive means to break through the skin barrier for efficient transdermal drug delivery. Here, we report the applications of solid polymer-based ion-conductive porous microneedles (PMN) containing interconnected micropores for improving iontophoresis, which is a technique of enhancing transdermal molecular transport by a direct current through the skin. The PMN modified with a charged hydrogel brings three innovative advantages in iontophoresis at once: (1) lowering the transdermal resistance by low-invasive puncture of the highly resistive stratum corneum, (2) transporting of larger molecules through the interconnected micropores, and (3) generating electroosmotic flow (EOF). In particular, the PMN-generated EOF greatly enhances the transdermal molecular penetration or extraction, similarly to the flow induced by external pressure. The enhanced efficiencies of the EOF-assisted delivery of a model drug (dextran) and of the extraction of glucose are demonstrated using a pig skin sample. Furthermore, the powering of the PMN-based transdermal EOF system by a built-in enzymatic biobattery (fructose / O2 battery) is also demonstrated as a possible totally organic iontophoresis patch.

    DOI: 10.1038/s41467-021-20948-4

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    Other Link: http://www.nature.com/articles/s41467-021-20948-4

  • Totally organic electrical skin patch powered by flexible biobattery Reviewed

    Shotaro Yoshida, Hiroya Abe, Yuina Abe, Shinya Kusama, Kenichi Tsukada, Ryo Komatsubara, Matsuhiko Nishizawa

    Journal of Physics: Energy   2 ( 4 )   044004   2020.10

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    An electrical skin patch that can be flexibly attached to the skin and activated in 30 s by adding water was developed by integrating a built-in flexible glucose/O(2)biobattery. The latter consisted of a glucose dehydrogenase (GDH)-modified anode and an iron(II) phthalocyanine (FePc)-modified cathode. The quick activation of the patch components by water addition deep inside the patch was achieved by using a flexible water-absorbing sponge containing glucose and buffer electrolyte. A patch current of about 10 mu A was maintained for more than 12 h by optimizing the amount of glucose and electrolyte contained in the sponge tank. The entire patch was soft and highly flexible to conform to curved skin surfaces, owing to its thinness (<2 mm) and the flexibility of all the patch components, including the enzyme electrodes based on the carbon fabric.

    DOI: 10.1088/2515-7655/abb873

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    Other Link: https://iopscience.iop.org/article/10.1088/2515-7655/abb873/pdf

  • Hydrogel-based sealed microchamber arrays for rapid medium exchange and drug testing of cell spheroids Reviewed

    Shotaro Yoshida, Kensuke Sumomozawa, Kuniaki Nagamine, Matsuhiko Nishizawa

    Biomedical Microdevices   22 ( 3 )   49   2020.9

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s10544-020-00505-4

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    Other Link: http://link.springer.com/article/10.1007/s10544-020-00505-4/fulltext.html

  • Totally Transparent Hydrogel-Based Subdural Electrode with Patterned Salt Bridge Reviewed International journal

    Ayaka Nishimura, Ryota Suwabe, Yuka Ogihara, Shotaro Yoshida, Hiroya Abe, Shin-ichiro Osawa, Atsuhiro Nakagawa, Teiji Tominaga, Matsuhiko Nishizawa

    Biomedical Microdevices   22 ( 3 )   57 - 57   2020.8

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    A totally transparent subdural electrode was developed by embedding a conductive poly (vinyl alcohol) (PVA)-filled microchannel made of poly(dimethylsiloxane) (PDMS) into an another PVA hydrogel substrate. Tight bonding between the PVA substrate and the PDMS microchannel (salt bridge) was achieved by mechanical interlocking utilizing the microprotrusions formed on the microchannel. This simple method of bonding without the use of any additives such as silane molecules or nanofibers is very suitable for constructing biomedical devices. The salt bridge electrode (total thickness, ca. 1.5 mm) was sufficiently soft, and showed superior shape conformability that makes it an excellent choice as a subdural electrode used on the brain surface. In vivo measurement proved that the salt bridge electrode makes close contact to the exposed porcine brain and can record brain wave signals of frequencies 1 ~ 15 Hz. In addition, the high transparency of the electrode provided a clear view of the brain surface that would assist the effective surgical operation and optogenetic research.

    DOI: 10.1007/s10544-020-00517-0

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  • Autonomous bioactuator driven by chicken cardiomyocytes

    Kaori Furuike, Ai Shima, Shotaro Yoshida, Yuya Morimoto, Shoji Takeuchi

    21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017   1441 - 1442   2020

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    © 17CBMS-0001. This paper describes a chick embryo cardiomyocyte actuator for investigation of cardiomyocyte contractile properties as alternatives of culture system with rodent cardiomyocytes. Chick embryo cardiomyocytes are more accessible than rat or mouse cardiomyocytes in terms of cost and ethics. We cultured chick embryo cardiomyocytes on parylene sheets and observed that the parylene sheets autonomously flapped with the contractile force of cardiomyocytes. Furthermore, the flap frequency increased in response to the addition of isoproterenol, which promotes contraction of cardiac muscles. This technique would reduce the cost of experiments, and thus be expected to promote the study of contractile properties of cardiomyocytes.

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  • Hydrogel-based transparent subdural electrode with ionic connection

    Ayaka Nishimura, Ryota Suwabe, Yuka Ogihara, Yuina Abe, Hiroya Abe, Shotaro Yoshida, Matsuhiko Nishizawa

    MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences   518 - 519   2020

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    We propose a hydrogel-based transparent subdural electrode with ionic connection for intraoperative recording brain waves (electrocorticography, ECoG). The present electrode was composed of a poly(dimethylsiloxane) (PDMS) microchannel filled with ionically conductive hydrogel (salt bridge) was embedded to a polyvinyl alcohol (PVA) hydrogel as substrate. The salt bridge acts as an ionic connection between the electrochemical interface and brain surface. The fabricated soft electrode showed high conformability to the curved wet surface of brains with optical transparency, which is an advantage for a subdural electrode. In vivo recording of porcine brain waves proved that the salt bridge electrode could be practical.

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  • Wearable patch-type device for bio-information monitoring with porous microneedle and flexible Ag/AgCl reference electrode

    Ryohei Takizawa, Yuina Abe, Bibek Raut, Hajime Konno, Natsumi Kimura, Shotaro Yoshida, Hiroya Abe, Matsuhiko Nishizawa

    MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences   504 - 505   2020

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    The monitoring of bio-information through skin with wearable devices has been attracted as an advanced mean for daily health management. We developed a patch-type wearable device composed of a novel porous microneedle (PMN) and a flexible, stable Ag/AgCl reference electrodes. The whole of PMN contains 3D microchannels, and serves as a minimally invasive interface for the transdermal transport of molecules and ions. By using the flexible PMN patch, we realized the monitoring of the transepidermal potential (TEP), a potential difference generated along the thickness direction of the epidermis, which is an useful indicator of skin health status.

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  • Series-Connected Flexible Biobatteries for Higher-Voltage Electrical Skin Patches Reviewed

    Shotaro Yoshida, Takaya Mizuno, Shinya Kusama, Kaito Sato, Bibek Raut, Matsuhiko Nishizawa

    ACS Applied Electronic Materials   2 ( 1 )   170 - 176   2019.12

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    DOI: 10.1021/acsaelm.9b00671

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  • Self-Moisturizing Smart Contact Lens Employing Electroosmosis Reviewed

    Shinya Kusama, Kaito Sato, Shotaro Yoshida, Matsuhiko Nishizawa

    Advanced Materials Technologies   5 ( 1 )   1900889   2019.11

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    DOI: 10.1002/admt.201900889

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  • Hydrogel-Based Organic Subdural Electrode with High Conformability to Brain Surface. Reviewed International journal

    Shuntaro Oribe*, Shotaro Yoshida*(*equal contribution), Shinya Kusama, Shin-Ichiro Osawa, Atsuhiro Nakagawa, Masaki Iwasaki, Teiji Tominaga, Matsuhiko Nishizawa

    Scientific reports   9 ( 1 )   13379 - 13379   2019.9

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    A totally soft organic subdural electrode has been developed by embedding an array of poly(3,4-ethylenedioxythiophene)-modified carbon fabric (PEDOT-CF) into the polyvinyl alcohol (PVA) hydrogel substrate. The mesh structure of the stretchable PEDOT-CF allowed stable structural integration with the PVA substrate. The electrode performance for monitoring electrocorticography (ECoG) was evaluated in saline solution, on ex vivo brains, and in vivo animal experiments using rats and porcines. It was demonstrated that the large double-layer capacitance of the PEDOT-CF brings low impedance at the frequency of brain wave including epileptic seizures, and PVA hydrogel substrate minimized the contact impedance on the brain. The most important unique feature of the hydrogel-based ECoG electrode was its shape conformability to enable tight adhesion even to curved, grooved surface of brains by just being placed. In addition, since the hydrogel-based electrode is totally organic, the simultaneous ECoG-fMRI measurements could be conducted without image artifacts, avoiding problems induced by conventional metallic electrodes.

    DOI: 10.1038/s41598-019-49772-z

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  • Red Light-Promoted Skin Barrier Recovery: Spatiotemporal Evaluation by Transepidermal Potential Reviewed

    Yuina Abe, Hajime Konno, Shotaro Yoshida, Takeshi Yamauchi, Kenshi Yamasaki, Mitsuhiro Denda, Matsuhiko Nishizawa

    PLoS ONE   14 ( 7 )   e0219198   2019.7

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    DOI: 10.1371/journal.pone.0219198

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  • Transepidermal Potential of the Stretched Skin Reviewed

    Yuina Abe, Hajime Konno, Shotaro Yoshida, Matsuhiko Nishizawa

    Journal of Biomechanical Engineering   141 ( 8 )   084503   2019.5

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    DOI: 10.1115/1.4043522

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  • Hydrogel Microchambers Integrated with Organic Electrodes for Efficient Electrical Stimulation of Human iPSC-derived Cardiomyocytes Reviewed

    Shotaro Yoshida, Kensuke Sumomozawa, Kuniaki Nagamine, Matsuhiko Nishizawa

    Macromolecular Bioscience   19 ( 6 )   1900060   2019.4

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    A hydrogel-based microchamber with organic electrodes for efficient electrical stimulations of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) is described. The microchamber is made from molecularly permeable, optically transparent, and electrically conductive polyvinyl alcohol (PVA) hydrogel and highly capacitive carbon electrode modified with poly(3,4-ethylenedioxythiophene) (PEDOT). Spheroids of hiPSC-CMs are cultured in microchambers, and electrically stimulated by the electrode for maturation. The large interfacial capacitance of the electrodes enables several days of electrical stimulation without generation of cytotoxic bubbles even when the electrodes are placed near the spheroids. The spheroids can be cultivated in the closed microchambers because of the permeated nutrients through the hydrogel, thus the spheroids are stably addressable and the culture medium around the sealed microchambers can be simply exchanged. Synchronized beating of the spheroids can be optically analyzed in situ, which makes it possible to selectively collect electrically responsive cells for further use. As the hydrogel is electrically conductive, the amount of electrical charge needed for maturing the spheroids can be reduced by configuring electrodes on the top and the bottom of the microchamber. The bioreactor will be useful for efficient production of matured hiPSC-CMs for regenerative medicine and drug screening.

    DOI: 10.1002/mabi.201900060

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  • 3D arrays of microcages by two-photon lithography for spatial organization of living cells. Reviewed International journal

    Florian Larramendy, Shotaro Yoshida, Daniela Maier, Zoltan Fekete, Shoji Takeuchi, Oliver Paul

    Lab on a chip   19 ( 5 )   875 - 884   2019.2

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    This paper addresses a nanoengineering approach to create a fully three-dimensional (3D) network of living cells, providing an advanced solution to in vitro studies on either neuronal networks or artificial organs. The concept of our work relies on stackable scaffolds composed of microcontainers designed and dimensioned to favor the geometrically constrained growth of cells. The container geometry allows cells to communicate in the culture medium and freely grow their projections to form a 3D arrangement of living cells. Scaffolds are fabricated using two-photon polymerization of IP-L 780 photoresist and are coated with collagen. They are stacked by mechanical micromanipulation. Technical details of the proposed nanofabrication scheme and assembly of the modular culture environment are explained. Preliminary in vitro results using PC12 cells have shown that this structure provides a good basis for healthy cell growth for at least 16 days. Our approach is envisioned to provide tailor-made solutions of future 3D cell assemblies for potential applications in drug screening or creating artificial organs.

    DOI: 10.1039/c8lc01240g

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  • Organic Electrochromic Timer for Enzymatic Skin Patches Reviewed

    Hiroyuki Kai, Wataru Suda, Shotaro Yoshida, Matsuhiko Nishizawa

    Biosensors and Bioelectronics   123 ( 1 )   108 - 113   2019.1

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    A totally organic and disposable electrochromic timer integrated with an enzymatic electrode and powered by biofuel cells is developed. The cathode of the self-powered electrochromic timer consists of a composite electrochromic film of poly(3,4-ethylenedioxythiophene) (PEDOT) and polyurethane (PU), while the anode is made up of a fructose dehydrogenase (FDH) enzymatic electrode. The electrochromic changes over time (up to 100 min) can be displayed in the device, and the speed of color change can be controlled by changing the resistance between the anode and the cathode. Automatic activation of the timer after placement on a skin is achieved by integrating a porous microneedle array. The electrochromic timer would be used along with a skin patch as a time-lapse display of medical and cosmetic treatments.

    DOI: 10.1016/j.bios.2018.07.013

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  • Multipoint Bending and Shape Retention of a Pneumatic Bending Actuator by a Variable Stiffness Endoskeleton Reviewed

    Yoshida, Shotaro, Morimoto, Yuya, Zheng, Lanying, Onoe, Hiroaki, Takeuchi, Shoji

    SOFT ROBOTICS   5 ( 6 )   718 - 725   2018.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MARY ANN LIEBERT, INC  

    We propose a pneumatic bending actuator integrated with a low-melting-point alloy-based variable stiffness endoskeleton that can bend at multiple points and maintain its bent shape without power supply. Local stiffness of the soft actuator can be altered by melting or hardening the endoskeleton with electric heat applied through embedded metal wires. Bending points of the actuator can be changed by selecting different points of the endoskeleton to be melted, and the bending angle can be controlled by injected air pressure. The shape of the bent actuator is maintained by hardening the alloy even when pressure is reduced to the initial state. We demonstrate that the actuator can be bent differently with only one pneumatic actuation layer by combining multipoint bending and the shape retention function, and thus the actuator can be used for lifting, holding, and unloading an object. We believe that the simple machinery of the actuator will be useful in programming complicated motions of soft robotic fingers, fins, and tentacles.

    DOI: 10.1089/soro.2017.0145

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  • Assembly and Connection of Micropatterned Single Neurons for Neuronal Network Formation Invited Reviewed

    Yoshida, Shotaro, Kato-Negishi, Midori, Takeuchi, Shoji

    MICROMACHINES   9 ( 5 )   235   2018.5

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI  

    Engineering of neuronal network geometry by micropatterning technology is a key future technology for creating artificial brains on a chip. However, engineering of network geometry at the single-cell-level with functional morphology (axon/dendrite) and connectivity (synapses) is still challenging. Here, we describe a method for controlling the axon and dendrite morphology of single primary-cultured neurons and assembling a neural circuit using mobile microplates. The microplates enabled morphological control of neurons by their shapes and bringing their ends into contact caused the formation of physical connections. Functional synapse formation at the connection was indicated by immunostaining of synapse-related proteins and intracellular Ca2+ imaging of neural activity. We believe that the method will be useful in engineering neural circuits with selected neurons and defined morphology.

    DOI: 10.3390/mi9050235

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  • Photolithographic patterned surface forms size-controlled lipid vesicles. Reviewed International journal

    M Gertrude Gutierrez, Shotaro Yoshida, Noah Malmstadt, Shoji Takeuchi

    APL bioengineering   2 ( 1 )   016104 - 016104   2018.3

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    Using traditional 2-D photolithographic methods, surface patterns are made on agarose and used to form lipid vesicles with controlled size and layout. Depending on the size and layout of the patterned structures, the lipid bilayer vesicle size can be tuned and placement can be predetermined. Vesicles formed on 2-D patterned surfaces can be harvested for further investigations or can be assayed directly on the patterned surface. Lipid vesicles on the patterned surface are assayed for unilamellarity and protein incorporation, and vesicles are indeed unilamellar as observed from outer leaflet fluorescence quenching. Vesicles successfully incorporate the integral membrane protein α-hemolysin and maintain its membrane transport function.

    DOI: 10.1063/1.5002604

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  • Hydrogel culture dish integrated with organic electrodes for efficient stimulation of hiPSC-derived cardiomyocytes

    Kensuke Sumomozawa, Kuniaki Nagamine, Hiroyuki Kai, Shotaro Yoshida, Hirokazu Kaji, Matsuhiko Nishizawa

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   3   1445 - 1447   2018

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    We propose a hydrogel-based bioreactor integrated with organic electrodes for efficient electrical stimulation of cardiomyocytes differentiated from human induced pluripotent stem cells (hiPSCs). The bioreactor was fabricated by integration of poly(3,4-ethylenedioxythiophene) (PEDOT)/polyurethane (PU) electrodes and a hydrogel with a cell-positioning chamber. The fabricated organic electrodes had a high capacity compared to conventional metal electrodes that prevented unfavorable electric polarization. Cardiomyocytes derived from hiPSCs were cultured in the bioreactor, and their beating frequency was synchronized to frequency of electrical stimulation applied by the electrodes. Efficient electrical stimulation was achieved by optimizing configuration of the electrodes.

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  • Surface modification for patterned cell growth on substrates with pronounced topographies using sacrificial photoresist and parylene-C peel-off Reviewed

    Larramendy, F., Yoshida, S., Jalabert, L., Takeuchi, S., Paul, O.

    Journal of Micromechanics and Microengineering   26 ( 9 )   095017   2016

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    DOI: 10.1088/0960-1317/26/9/095017

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  • Mobile Microplates for Morphological Control and Assembly of Individual Neural Cells Reviewed

    Yoshida, S., Teshima, T., Kuribayashi-Shigetomi, K., Takeuchi, S.

    Advanced Healthcare Materials   5 ( 4 )   415 - 420   2016

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    DOI: 10.1002/adhm.201500782

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  • Vertical Flow Lithography for Fabrication of 3D Anisotropic Particles Reviewed

    Shohei Habasaki, Won Chul Lee, Shotaro Yoshida, Shoji Takeuchi

    SMALL   11 ( 48 )   6391 - 6396   2015.12

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    DOI: 10.1002/smll.201502968

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  • Liquid-filled tunable lenticular lens Reviewed

    Yoshinobu Iimura, Hiroaki Onoe, Tetsuhiko Teshima, Yun Jung Heo, Shotaro Yoshida, Yuya Morimoto, Shoji Takeuchi

    JOURNAL OF MICROMECHANICS AND MICROENGINEERING   25 ( 3 )   035030   2015.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:IOP PUBLISHING LTD  

    This paper describes a liquid-filled tunable lenticular lens for switching between two-dimensional (2D) and three-dimensional (3D) images in naked-eye 3D displays. Compared with previous 2D/3D switchable displays, this tunable lenticular lens that is directly attached to a smartphone display can project both a 2D image with the original resolution of the smartphone display and a 3D image with high brightness. This lens is simply composed of transparent poly(dimethylsiloxane) (PDMS) microchannels. While the thin top membrane on the microchannels is normally flat to transmit light without deflection for displaying 2D images, applying pressure to the microchannel deforms the membrane to acquire characteristics of lenticular lenses for 3D images. We successfully demonstrate the switching between the 2D and 3D modes. We believe that our lens can be applied as a part of a portable 2D/3D naked-eye 3D display.

    DOI: 10.1088/0960-1317/25/3/035030

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  • Three-Dimensional Microassembly of Cell-Laden Microplates by in situ Gluing with Photocurable Hydrogels. Reviewed

    Shotaro Yoshida, Koji Sato, Shoji Takeuchi

    International Journal of Automation Technology   8 ( 1 )   95 - 101   2014

  • Relationship between phase and amplitude generalization errors in complex- and real-valued feedforward neural networks Reviewed

    Akira Hirose, Shotaro Yoshida

    NEURAL COMPUTING & APPLICATIONS   22 ( 7-8 )   1357 - 1366   2013.6

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER  

    We compare the generalization characteristics of complex-valued and real-valued feedforward neural networks. We assume a task of function approximation with phase shift and/or amplitude change in signals having various coherence. Experiments demonstrate that complex-valued neural networks show smaller generalization error than real-valued networks having doubled input and output neurons in particular when the signals have high coherence, that is, high degree of wave nature. We also investigate the relationship between amplitude and phase errors. It is found in real-valued networks that abrupt change in amplitude is often accompanied by steep change in phase, which is a consequence of local minima in real-valued supervised learning.

    DOI: 10.1007/s00521-012-0960-z

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  • Mobile miroplates for handling morphologically controlled single neural cells

    S. Yoshida, T. Teshima, K. Kuribayashi-Shigetomi, S. Takeuchi

    17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013   3   1986 - 1988   2013

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    This paper describes mobile microplates for morphological control and handling of single neural cells. We fabricated mobile microplates having a center circle (termed "Base") and radiated lines (termed "Path") for controlling the shape of neural cells: cell bodies and neurites. Single neural cells were cultured on the microplates, and their morphologies were fully controlled by the Base/Path structures. The morphologically controlled single neural cells on the microplates were also three-dimensionally handlable by microtweezers. We believe our method would be useful in the assembly of cultured neuronal networks at the single cell level.

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  • Generalization Characteristics of Complex-Valued Feedforward Neural Networks in Relation to Signal Coherence Reviewed

    Akira Hirose, Shotaro Yoshida

    IEEE TRANSACTIONS ON NEURAL NETWORKS AND LEARNING SYSTEMS   23 ( 4 )   541 - 551   2012.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC  

    Applications of complex-valued neural networks (CVNNs) have expanded widely in recent years-in particular in radar and coherent imaging systems. In general, the most important merit of neural networks lies in their generalization ability. This paper compares the generalization characteristics of complex-valued and real-valued feedforward neural networks in terms of the coherence of the signals to be dealt with. We assume a task of function approximation such as interpolation of temporal signals. Simulation and real-world experiments demonstrate that CVNNs with amplitude-phase-type activation function show smaller generalization error than real-valued networks, such as bivariate and dual-univariate real-valued neural networks. Based on the results, we discuss how the generalization characteristics are influenced by the coherence of the signals depending on the degree of freedom in the learning and on the circularity in neural dynamics.

    DOI: 10.1109/TNNLS.2012.2183613

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  • Photo-assisted micro-gluing for assembling three-dimensional microstructures with living cells

    Shotaro Yoshida, Koji Sato, Kaori Kuribayashi-Shigetomi, Tetsuhiko Teshima, Shoji Takeuchi

    Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012   509 - 511   2012

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    This paper describes a method to assemble three-dimensional (3D) microstructures by gluing separated two-dimensional (2D) structures attaching with living cells at micron-scales using hydrogels. We utilized photo-curable, biocompatible poly(ethylene)-glycol-diacrylate (PEGDA) as a glue, and assembled 2D cell-laden microplates into 3D structures by photoassisted local polymerizations. We demonstrated an assembly of 3D half-cubic microstructures with genetically-labeled cells, which enabled observing intracellular microstructure in 3D cellular constructs. We believe our method is useful for assembling 3D structures with targeted cells and into arbitrary geometries.

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  • 3F1322 Single Neurons on Mobile Microplates for Designing Neuronal Circuits(3F Neuroscience & Sensory systems, Neuronal Circuit & Information processing,The 49th Annual Meeting of the Biophysical Society of Japan)

    Yoshida Shotaro, Teshima Tetsuhiko, Kuribayashi-Shigetomi Kaori, Takeuchi Shoji

    Seibutsu Butsuri   51   S127   2011

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    Language:English   Publisher:The Biophysical Society of Japan General Incorporated Association  

    DOI: 10.2142/biophys.51.S127_1

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  • Single neural cells on mobile microplates for precise neural network assembly

    S. Yoshida, T. Teshima, K. Kuribayashi-Shigetomi, S. Takeuchi

    15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011   3   1749 - 1751   2011

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    This paper describes a method to assemble single neural cells into neural networks by utilizing microfabricated mobile microplates. Single neural cells were cultured on the mobile microplates. Directions of their neurite growth were controlled by guidepaths attached beside the microplates. We demonstrated that single neural cells with directed neurites were moved and assembled by mobile microplates into desired network structure, and also the network geometry was redefined. We believe this modular assembly method could be applied to precise patterning of neural networks, and also useful in basic studies of single-cell-level neural networks.

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Books

  • 有機材料の微細加工による生体親和性電気刺激・計測システムの開発

    村上 達哉, 吉田 昭太郎( Role: Joint author精密工学会誌, 89巻9号 通巻1065号, pp.678-680)

    公益社団法人 精密工学会  2023.9 

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  • ハイドロゲルを基材とする生体親和性の有機物電極

    吉田昭太郎, 中川敦寛, 西澤松彦( Role: Joint authorバイオマテリアル-生体材料- 39巻3号 pp.176-181)

    日本バイオマテリアル学会  2021.7 

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  • Enzyme Transducers and Evolutions of Enzymatic Technology―Enzyme Sensors, Bio Batteries and Novel Enzymatic Approaches (Food, Medicine, Restoring) ―

    ( Role: Contributor)

    2020.3 

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  • Hydrogel-Based Organic Electrodes for Biocomfortable Electrocorticography

    Shotaro Yoshida, Matsuhiko Nishizawa( Role: Contributor)

    2020.3 

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  • 微細ポリマー構造を用いた一細胞の培養と操作 (日本バイオマテリアル学会誌)

    吉田 昭太郎, 竹内昌治( Role: Joint author第35巻1号)

    日本バイオマテリアル学会  2017 

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  • マイクロ・ナノデバイスによる膜系システムの理解 (人工細胞の創製とその応用)

    吉田 昭太郎, 神谷厚輝, 竹内昌治( Role: Contributor第二章)

    シーエムシー出版  2017 

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  • Protein patterning by parylene-C peel-off process

    LARRAMENDY Florian, YOSHIDA Shotaro, JALABERT Laurent, FUJITA Hiroyuki, TAKEUCHI Shoji, PAUL Oliver( Role: Joint author)

    Institute of Industrial Science The University of Tokyo  2015 

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    Language:English   Book type:Scholarly book

    This paper introduces a new technique for patterning functionalization layers on substrates with hightopogra phy. The method is based on a parylene-C template shaped by a structured, sacrificial photoresist layer and attached to the substrate where functionalization is not intended. After photoresist removal and surface function ali za tion, the parylene layer is peeled off, leaving all areas initially covered by the sacrificial polymer functionalized. The technique has several advantages: (i) In contrast to microcontact printing, it allows surfaces with complex topographies to be functionalized; (ii) complex function al ization patterns are possible; (iii) the parylene structure can be reutilized. We successfully demonstrate the technique with the guided growth of neuron-like PC12 cells on honeycomb-shaped protein patterns on micro pillars and microwells. The range and limits of the technique are analyzed and discussed in detail.

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  • Fabrication of 3D Cellular Tissue Utilizing MEMS Technologies (Hyper Bio Assembler for 3D Cellular Systems)

    Shotaro Yoshida, Daniela Serien, Fumiaki Tomoike, Hiroaki Onoe, Shoji Takeuchi( Role: ContributorChapter 10 (pp. 177-202))

    Springer Japan  2015 

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MISC

  • Centrifuge-based membrane emulsification toward high-throughput generation of monodisperse liposomes

    Dongchel Shin, Shotaro Yoshida, Yuya Morimoto, Shoji Takeuchi

    TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems   123 - 126   2017.7

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    Language:English   Publisher:Institute of Electrical and Electronics Engineers Inc.  

    In this paper, we developed a centrifugal emulsification device toward high-throughput generation of monodisperse liposomes. The device generated lipid vesicles in two steps, membrane emulsification by asymmetric through-holes on a plate, and transfer of the emulsions to a planar monolayer at an oil/water interface. As a result of centrifugation of the device in a microtube, we succeeded in collection of cell-sized lipid vesicle. Since a number of the asymmetric through-holes can be fabricated on the plate by standard photolithographic process, and the reservoir for inner solution has relatively large volume (40 μl), the proposed device will be a powerful tool for massive generation of liposomes.

    DOI: 10.1109/TRANSDUCERS.2017.7994003

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  • Single Cell Culture and Manipulation Using Microfabricated Polymeric Structures

    吉田 昭太郎, 竹内 昌治

    バイオマテリアル = Journal of Japanese Society for Biomaterials : 生体材料   35 ( 1 )   46 - 49   2017.1

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    Language:Japanese   Publisher:日本バイオマテリアル学会 ; 2002-  

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  • CELLS SMELL ON A CMOS: A PORTABLE ODORANT DETECTION SYSTEM USING CELL-LADEN COLLAGEN PILLARS

    Yusuke Hirata, Yuya Morimoto, Eunryel Nam, Shotaro Yoshida, Shoji Takeuchi

    30TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2017)   13 - 16   2017

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    We developed a portable odorant detection system composed of collagen micro-pillars with cells expressing olfactory receptors and a CMOS imaging sensor; the CMOS imaging sensor caught fluorescence signals of Ca2+ increased by odorant molecules. While low sensitiveness of the CMOS imaging sensor was insufficient to detect the fluorescent signal from a single cell, the odorant detection system achieved supply of sufficient odorant-induced fluorescence intensity to the CMOS imaging sensor by vertical accumulation of the fluorescence from each cells in the pillars. We believe that our portable odorant detection system will be a useful platform for quantitative and selective portable odorant sensor.

    DOI: 10.1109/MEMSYS.2017.7863327

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  • Single neuron microplate for morphology control and assembly of primary neurons

    33   1 - 4   2016.10

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  • 3D assembly of fiber-based tissues combined with micro pillar connectors

    Kaori Furuike, Yuya Morimoto, Shotaro Yoshida, Nobuhito Mori, Shoji Takeuchi, Shoji Takeuchi

    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016   413 - 414   2016.1

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    We propose a method to assemble fiber-shaped tissues into a 3D network. In the method, we connected fiber-shaped tissues using micro pillar devices anchoring both ends of the tissues. The fibershaped tissues were assembled into heterogeneous and 3D network. We believe that this method will be useful for the analysis of cellular morphology and functions in a 3D network of tissues.

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  • Spheroids on a CMOS chip for a portable odorant sensor

    Yusuke Hirata, Shotaro Yoshida, Seiji Tabata, Eunryel Nam, Eunryel Nam, Yuya Morimoto, Shoji Takeuchi, Shoji Takeuchi

    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016   1214 - 1215   2016.1

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    We propose a fluorescence detection system for a portable odorant biosensor composed of cell spheroids with expressed olfactory receptors and a CMOS chip. As fluorescence intensity of spheroids is higher than that of single cells, we achieved detection of fluorescence signals caused by intracellular chemical reaction on the CMOS chip. As a demonstration of odorant detection, we prepared spheroids of cells expressing olfactory receptors to certain odorant molecules. The CMOS chip could detect the increment of the fluorescence intensity around the spheroids when odorant molecules were applied. We believe that the system will contribute to establish portable odorant biosensors.

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  • CATCH A CELL ON A CMOS: SELECTIVE RETRIEVAL OF SINGLE CELL USING A MICROPLATE TECHNOLOGY PERFORMED ON A CMOS IMAGING SENSOR

    Seiji Tabata, Shotaro Yoshida, Yuya Morimoto, Shoji Takeuchi

    2016 IEEE 29TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS)   2016-February   489 - 492   2016

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    This paper reports a system enabling cell detection, fluorescence light detection and single cell retrieval. It consists of a device combining a microplate technology, allowing single cell seeding on individual cell-sized paiylene plates, and a CMOS imaging sensor placed on the right bottom of the microplate chip with glass substrate. By using such a system, we succeeded to visualize individual cells, to detect fluorescence light emission and to retrieve a single cell by using a micromanipulator. We believe that the proposed system will be useful for the point of care analysis on the level of single cell analysis.

    DOI: 10.1109/MEMSYS.2016.7421668

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  • Protein patterning by parylene-C peel-off process

    LARRAMENDY Florian, YOSHIDA Shotaro, JALABERT Laurent, FUJITA Hiroyuki, TAKEUCHI Shoji, PAUL Oliver

    SEISAN KENKYU   67 ( 5 )   521 - 524   2015

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    Language:English   Publisher:Institute of Industrial Science The University of Tokyo  

    This paper introduces a new technique for patterning functionalization layers on substrates with hightopogra phy. The method is based on a parylene-C template shaped by a structured, sacrificial photoresist layer and attached to the substrate where functionalization is not intended. After photoresist removal and surface function ali za tion, the parylene layer is peeled off, leaving all areas initially covered by the sacrificial polymer functionalized. The technique has several advantages: (i) In contrast to microcontact printing, it allows surfaces with complex topographies to be functionalized; (ii) complex function al ization patterns are possible; (iii) the parylene structure can be reutilized. We successfully demonstrate the technique with the guided growth of neuron-like PC12 cells on honeycomb-shaped protein patterns on micro pillars and microwells. The range and limits of the technique are analyzed and discussed in detail.

    DOI: 10.11188/seisankenkyu.67.521

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  • CELL-LADEN HINGED MICROPLATES FOR MEASURING THE CONTRACTILE FORCES OF CARDIOMYOCYTES

    Hiroaki Matsumoto, Shotaro Yoshida, Yuya Morimoto, Nobuhito Mori, Daniela Serien, Shoji Takeuchi

    2015 28TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2015)   2015-February   589 - 592   2015

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    We report a method to measure contractile forces of cardiomyocytes at cellular level using microplates; pairs of single-cell sized plates are connected by a flexible hinge at the center. Cardiomyocytes repeatedly contract and relax, and thereby fold and stretch the microplates at the flexible hinge. By measuring the change of the bending angle in folded microplates, we can estimate contractile forces of cardiomyocytes. We believe that this method is a useful tool to study the biomechanics of cardiomyocytes.

    DOI: 10.1109/MEMSYS.2015.7051024

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  • Fabrication of 3D Dynamic Microarray Device By Using a 3D Printer

    31   1 - 4   2014.10

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    Language:Japanese   Publisher:Institute of Electrical Engineers of Japan  

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  • Pneumatic Balloon Actuators with Tunable Bending Points

    31   1 - 4   2014.10

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  • Modular assembly of neural circuit using a single neural cell on a microplate

    31   1 - 4   2014.10

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  • Centrifuge-based microfluidic device for trapping single cells in a drop

    Hiromasa Hasegawa, Shotaro Yoshida, Yuya Morimoto, Yuya Morimoto, Shoji Takeuchi, Shoji Takeuchi

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014   1410 - 1412   2014.1

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    © 14CBMS. We describe a centrifuge-based dynamic microarray system for trapping an array of single cells from small amount of suspension. We optimized microchannel designs and centrifugal force for minimizing sample volumes and maximizing trapping efficiencies, and achieved single-cell trapping by introducing only 5 μl of cell suspension with the centrifugal force of 70 G. Trapping of cells stained with DAPI shows that single cells were trapped at desired trapping spots. This system will be applicable for single cell assays using rare or expensive cells.

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  • Mobile Microplates for Isolation Culture, Morphological Control, and Three-dimensional Manipulation of Single Neural Cells

    30   1 - 4   2013.11

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  • FABRICATION OF MOUNTAIN FOLDS IN CELL ORIGAMI BY CONJOINING VALLEY FOLDS

    30   4p   2013.11

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  • 6PM3-PMN-002 THREE-DIMENSIONAL FABRICATION OF MICROSTRUCTURES USING VERTICAL CONTINUOUS FLOW LITHOGRAPHY

    Habasaki Shohei, Yoshida Shotaro, Lee Won Chul, Takeuchi Shoji

    2013 ( 5 )   177 - 178   2013.11

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    Language:Japanese   Publisher:The Japan Society of Mechanical Engineers  

    We achieved three-dimensional (3D) fabrication of heterogeneous microstructures using an in situ photo-polymerization method, vertical continuous flow lithography (VCFL). Utilizing a projection system including a Digital Mirror Device, 3D microstructures were fabricated in a layer-by-layer fashion with the thickness of each layer controlled by UV absorber and exposure time. In addition, VCFL fabricated heterogeneous microstructures by exposing light patterns to multiple laminar flows. We demonstrated three-layered structures and long fibers whose cross-sectional geometries were changed. We also fabricated two-layered Janus structures. We believe that VCFL will be applicable to making anisotropic microparticles for material science and bottom up tissue engineering.

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  • Microfluidically tunable lenticular lens

    Y. Iimura, T. Teshima, Y. J. Heo, Y. Morimoto, S. Yoshida, H. Onoe, S. Takeuchi

    2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013   1787 - 1790   2013

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    This paper describes a tunable lenticular lens to switch high-resolution two-dimensional (2D) / three-dimensional (3D) images in naked-eye stereoscopic displays. This lens is simply composed of transparent poly(dimethylsiloxane) (PDMS) microchannels and pumps to realizes two states: (A) flat surface for light transmissions without deflection
    (B) cylindrically deformed surface for light deflection. This lens has three advantages: (i) switchable optical characteristics without patterned electrode, (ii) adjustable focal length by the pressure-driven deformation, (iii) simple fabrication and low-cost experimental setup. By using this device put on a Smart Phone display, we successfully observed 3D images and two different images depending on view angle. Moreover, we obtained clear 2D images without decrease in the horizontal resolution. We believe that this device can be applied to a portable and high luminance naked-eye stereoscopic display by the combination with various types of commercially available portable displays. © 2013 IEEE.

    DOI: 10.1109/Transducers.2013.6627135

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  • Generalization characteristics of complex-valued neural networks in dealing with wave-related data

    HIROSE Akira, YOSHIDA Shotaro

    IEICE technical report. Neurocomputing   111 ( 483 )   81 - 86   2012.3

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    Language:Japanese   Publisher:The Institute of Electronics, Information and Communication Engineers  

    This paper compares the generalization characteristics of complex-valued and real-valued feedforward neural networks in terms of coherence of signals to be dealt with. We assume a task of function approximation such as interpolation of temporal signals. Simulation and real-world experiments demonstrate that complex-valued neural networks with amplitude-phase-type activation function show smaller generalization error than real-valued networks such as bivariate and dual-univariate real-valued neural networks. Based on the results, we discuss how the generalization characteristics are influenced by the coherence of the signals depending on the degree of freedom in the learning and on the circularity in neural dynamics.

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  • Comparison of Complex- and Real-Valued Feedforward Neural Networks in Their Generalization Ability

    Akira Hirose, Shotaro Yoshida

    NEURAL INFORMATION PROCESSING, PT I   7062   526 - +   2011

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    We compare the generalization characteristics of complex-valued and real-valued feedforward neural networks when they deal with wave-related signals. We assume a task of function approximation. Experiments demonstrate that complex-valued neural networks show smaller generalization error than real-valued ones in particular when the signals have high degree of wave nature.

    DOI: 10.1007/978-3-642-24955-6_63

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Presentations

  • 有機電子材料を活用した高生体親和性ウェアラブル・インプランタブルデバイス Invited

    吉田昭太郎

    ヘルスケア・医療機器専門技術研究会 第 3 回研究会  2024.1 

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  • 単一神経細胞の制御と可動化のためのマイクロハイドロゲルアレイの開発

    礎 慎太郎, 畑 翔太, 兒玉 泰河, 吉田 昭太郎

    第13回マイクロ・ナノ工学シンポジウム  2022.11 

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  • 有機物のみで構成されたダイオードの開発

    中谷 翔大, 原 悠真, 川上 哲, 吉田 昭太郎

    第13回マイクロ・ナノ工学シンポジウム  2022.11 

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  • マイクロニードルによる表皮デバイスと皮膚内液との結合

    小山 峻輝, 船越 耀介, 児玉 泰河, 吉田 昭太郎

    第13回マイクロ・ナノ工学シンポジウム  2022.11 

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  • 有機物電子素子を使用した無線電力伝送

    川上 哲, 吉田 昭太郎

    第13回マイクロ・ナノ工学シンポジウム  2022.11 

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  • 塩水のみで駆動するハイドロゲル電池の 2 次電池利用の検討

    中山海斗, 吉田昭太郎, 鈴木宏明

    化学とマイクロ・ナノシステム学会 第46回研究会(CHEMINAS 50)  2022.11 

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  • 有機材料のみを用いた屈曲点の硬さ制御が可能なソフトロボットの構築

    船越 耀介, 吉田 昭太郎

    第13回マイクロ・ナノ工学シンポジウム  2022.11 

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  • 培養神経回路への局所的な薬剤投与のための有機電子イオンポンプの製作

    児玉 泰河, 礎 慎太郎, 吉田 昭太郎

    第13回マイクロ・ナノ工学シンポジウム  2022.11 

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  • 有機材料を中心とした導電性高分子アクチュエータシステムの構築

    佐々木 海, 吉田 昭太郎

    第13回マイクロ・ナノ工学シンポジウム  2022.11 

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  • 血管網デバイスを用いた内皮間葉移行および 口腔癌の転移メカニズムの理解

    土屋香碧, 梨本裕司, 堀武志, 吉田昭太郎, 渡部, 徹郎,梶弘和

    化学とマイクロ・ナノシステム学会 第46回研究会(CHEMINAS 50)  2022.11 

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  • マイクロメッシュシートを利用した三次元細胞培養法の開発

    安藤萌, 堀武志, 山本茜, 北野勇, 石原甲平, 水田太郎, 長濱宏治, 吉田昭太郎, 梨本裕司, 梶弘和

    化学とマイクロ・ナノシステム学会 第46回研究会(CHEMINAS 50)  2022.11 

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  • 有機電子回路のための印刷型有機薄膜トランジスタの開発

    原 悠真, 吉田 昭太郎

    第13回マイクロ・ナノ工学シンポジウム  2022.11 

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  • バイオ電池を用いた投薬パッチの製作

    南波 英途, 佐々木 海, 小山 峻輝, 吉田 昭太郎

    第13回マイクロ・ナノ工学シンポジウム  2022.11 

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  • グリア細胞を一細胞単位で操作可能なマイクロアレイデバイスの開発

    畑 翔太, 礎 慎太郎, 吉田 昭太郎

    第13回マイクロ・ナノ工学シンポジウム  2022.11 

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  • 神経回路の一細胞レベル電位計測のためのマイクロ有機電極アレイセンサの開発

    彌田 尚希, 原 悠真, 村上 達哉, 吉田 昭太郎

    第13回マイクロ・ナノ工学シンポジウム  2022.11 

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  • 指先の温度を感知・制御可能な有機ウェアラブルデバイスの開発

    土屋香碧, 吉田昭太郎

    化学とマイクロ・ナノシステム学会 第45回研究会(CHEMINAS 45)  2022.5 

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  • グリア細胞を一細胞単位で培養可能なマイクロアレイデバイスの開発

    畑翔太, 礎慎太郎, 吉田昭太郎

    化学とマイクロ・ナノシステム学会 第45回研究会(CHEMINAS 45)  2022.5 

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  • 神経回路の一細胞レベル電位計測のためのマイクロ電極アレイセンサの開発

    彌田尚希, 吉田昭太郎

    化学とマイクロ・ナノシステム学会 第45回研究会(CHEMINAS 45)  2022.5 

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  • 有機材料のみからなる電子回路の構築に向けた有機トランジスタの開発

    原悠真, 吉田昭太郎

    化学とマイクロ・ナノシステム学会 第45回研究会(CHEMINAS 45)  2022.5 

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  • グリア細胞の化学物質分泌センシングに向けた有機電気化学センサーの開発

    安藤萌, 吉田昭太郎

    化学とマイクロ・ナノシステム学会 第45回研究会(CHEMINAS 45)  2022.5 

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  • 炭素電極を用いた生体誘電率計測

    影山 湧二, 吉田昭太郎, 村上慎吾

    第69回応用物理学会春季学術講演会  2022.3 

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  • 気化による体積膨張を用いた任意の点で屈曲可能なソフトロボットアクチュエータの構築

    船越 耀介, 吉田 昭太郎

    第12回マイクロ・ナノ工学シンポジウム  ( オンライン )   2021.11  日本機械学会

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  • マイクロデバイスを用いたヒト iPS 神経細胞パターニング技術の開発

    児玉 泰河, 吉田 昭太郎

    第12回マイクロ・ナノ工学シンポジウム  ( オンライン )   2021.11  日本機械学会

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  • 単一神経細胞の制御のためのマイクロプレートデバイスの開発

    礎 慎太郎, 吉田 昭太郎

    第12回マイクロ・ナノ工学シンポジウム  ( オンライン )   2021.11  日本機械学会

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  • 導電性高分子アクチュエーターを駆動するための酵素型バイオ燃料電池の開発

    佐々木 海, 吉田 昭太郎

    第12回マイクロ・ナノ工学シンポジウム  ( オンライン )   2021.11  日本機械学会

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  • 有機物電子素子によるフレキシブル共振回路の作製

    川上 哲, 吉田 昭太郎

    第12回マイクロ・ナノ工学シンポジウム  ( オンライン )   2021.11  日本機械学会

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  • Electrical biosensing and biomodulation device based on organic/bio materials Invited

    2021.9 

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  • 有機材料を基盤とする生体親和性ウェアラブル/インプランタブル電子デバイスの開発 Invited

    吉田 昭太郎

    化学とマイクロ・ナノシステム学会 第43回研究会(CHEMINAS 43)若手優秀賞講演  2021.5 

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  • Hydrogel-based transparent subdural electrode with ionic connection

    Ayaka Nishimura, Ryota Suwabe, Yuka Ogihara, Yuina Abe, Hiroya Abe, Shotaro Yoshida, Matsuhiko Nishizawa

    MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences  2020 

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  • Wearable patch-type device for bio-information monitoring with porous microneedle and flexible Ag/AgCl reference electrode

    Ryohei Takizawa, Yuina Abe, Bibek Raut, Hajime Konno, Natsumi Kimura, Shotaro Yoshida, Hiroya Abe, Matsuhiko Nishizawa

    MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences  2020 

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  • 皮膚機能の電気的評価・制御デバイスの創成

    阿部結奈,今野創,瀧沢凌平,吉田昭太郎,山内丈史,山崎研志,西澤松彦

    第32回バイオエンジニアリング講演会  2019.12 

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  • ハイドロゲル電極を用いた生体電気刺激・計測システムの開発 Invited

    吉田昭太郎

    第50回セミコンファレンス 第32回東北若手の会  2019.12 

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  • スキンパッチ用ポーラスマイクロニードルの開発

    松井裕矢、草間慎也、木村菜摘、瀧沢凌平、吉田昭太郎、西澤松彦

    第50回セミコンファレンス 第32回東北若手の会  2019.12 

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  • 生体用電極のための塩橋型ハイドロゲル電極の開発

    西村文花, 諏訪部椋大, 荻原由佳, 吉田昭太郎, 西澤松彦

    第50回セミコンファレンス 第32回東北若手の会  2019.12 

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  • 経皮流体輸送のための皮膚パッチの開発

    佐藤開人, 草間慎也, 水野貴也, 木村菜摘, 吉田昭太郎, 西澤松彦

    第50回セミコンファレンス 第32回東北若手の会  2019.12 

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  • スキンパッチ用ポーラスマイクロニードルの開発

    松井裕矢、草間慎也、木村菜摘、瀧沢凌平、吉田昭太郎、西澤松彦

    第29回 日本MRS年次大会  2019.11 

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  • バイオインターフェイスのためのハイドロゲル塩橋アレイの開発

    西村文花, 諏訪部椋大, 荻原由佳, 吉田昭太郎, 西澤松彦

    第29回 日本MRS年次大会  2019.11 

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  • 経皮流動制御パッチの開発

    佐藤開人, 草間慎也, 水野貴也, 木村菜摘, 吉田昭太郎, 西澤松彦

    第29回 日本MRS年次大会  2019.11 

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  • 経皮流体輸送のための皮膚パッチの開発

    佐藤開人, 草間慎也, 水野貴也, 木村菜摘, 吉田昭太郎, 西澤松彦

    化学とマイクロ・ナノシステム学会第40回研究会  2019.11 

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  • スキンパッチ用ポーラスマイクロニードルの開発

    松井裕矢, 草間慎也, 木村菜摘, 瀧沢凌平, 吉田昭太郎, 西澤松彦

    化学とマイクロ・ナノシステム学会第40回研究会  2019.11 

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  • バイオインターフェースのための塩橋型ハイドロゲル電極の開発

    西村文花, 諏訪部椋大, 荻原由佳, 吉田昭太郎, 西澤松彦

    化学とマイクロ・ナノシステム学会第40回研究会  2019.11 

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  • 微小針塩橋を用いる皮膚機能の電気的評価・制御デバイスの開発

    阿部結奈,今野創,瀧沢凌平,吉田昭太郎,山内丈史,山崎研志,西澤松彦

    化学とマイクロ・ナノシステム学会第40回研究会  2019.11 

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  • ORGANIC TRANSDERMAL IONTOPHORESIS PATCH POWERED BY SERIALIZED LAYER-BUILT BIOFUEL CELLS

    Takaya Mizuno, Kaito Sato, Shinya Kusama, Shotaro Yoshida, Matsuhiko Nishizawa

    The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2019)  2019.10 

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  • HYDROGEL-BASED SEALED MICROCHAMBERS FOR SIMPLE AND COST- EFFECTIVE CELL CULTURE AND DRUG TESTING

    Shotaro Yoshida, Kensuke Sumomozawa, Matsuhiko Nishizawa

    The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2019)  2019.10 

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  • LIGHT THERAPY DEVICE WITH TRANSEPIDERMAL POTENTIAL-BASED REAL-TIME MONITORING OF SKIN BARRIER RECOVERY

    The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2019)  2019.10 

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  • ENGINEERED 3D ELECTROOSMOTIC MICROCHANNELS FOR RAPID AND MASS TRANSPORTATION OF BODY FLUIDS IN WEARABLE DEVICES

    The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2019)  2019.10 

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  • 皮膚通電パッチとしての酵素バイオ電池の分割・直列接続による高電圧化

    水野 貴也, 草間 慎也, 佐藤 開人, 吉田 昭太郎, 西澤 松彦

    第80回応用物理学会秋季学術講演会  2019.9 

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  • 抗ドライアイ機能を有する電気浸透流コンタクトレンズの開発

    草間 慎也, 佐藤 開人, 吉田 昭太郎, 西澤 松彦

    第80回応用物理学会秋季学術講演会  2019.9 

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  • 電気的バリア機能評価を基盤とする皮膚セラピーデバイスの開発

    今野 創, 阿部 結奈, 吉田 昭太郎, 西澤 松彦

    第80回応用物理学会秋季学術講演会  2019.9 

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  • イオントフォレシス型経皮流動制御パッチの開発

    佐藤開人, 草間慎也, 吉田昭太郎, 西澤松彦

    日本機械学会東北支部 東北学生会 第 49回 卒業研究発表講演会  2019.3 

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  • 経皮通電のためのポーラスマイクロニードルの開発

    松井裕矢, 熊田裕希, 水野貴也, 吉田昭太郎, 西澤松彦

    日本機械学会東北支部 東北学生会 第 49回 卒業研究発表講演会  2019.3 

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  • Development of an organic coil for transcutaneous energy transfer in a biostimulation device

    2019.3 

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  • 皮膚パッチに適用するための酵素バイオ燃料電池の最適化

    水野貴也, 豊里涼馬, 佐藤開人, 熊田裕希, 松井裕矢, 吉田昭太郎, 西澤松彦

    第28回 MRS-Japan 2018  2018.12 

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  • Effective Electroosmosis in Designed Polymeric Materials

    草間慎也, 豊里涼馬, 佐藤開人, 吉田昭太郎, 西澤松彦

    第28回 MRS-Japan 2018  2018.12 

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  • 皮膚イオニクスのための表皮電位測定プローブの開発

    今野創, 阿部結奈, 吉田昭太郎, 西澤松彦

    第28回 MRS-Japan 2018  2018.12 

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  • 電気浸透の医工学応用に向けた高分子材料の設計

    草間慎也, 豊里涼馬, 佐藤開人, 熊田裕希, 松井裕矢, 吉田昭太郎, 西澤松彦

    第49回セミコンファレンス、第31回東北若手の会  2018.11 

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  • 皮膚イオニクスのための小型表皮電位測定プローブの開発と応用

    今野創, 阿部結奈, 吉田昭太郎, 西澤松彦

    第49回セミコンファレンス、第31回東北若手の会  2018.11 

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  • Hydrogel culture dish integrated with organic electrodes for efficient stimulation of hiPSC-derived cardiomyocytes

    Kensuke Sumomozawa, Kuniaki Nagamine, Hiroyuki Kai, Shotaro Yoshida, Hirokazu Kaji, Matsuhiko Nishizawa

    MicroTAS2018  2018.11 

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  • 汗成分のモニタリングを目指した液滴収集フィルムの開発

    豊里 涼馬、甲斐 洋行、吉田 昭太郎、西澤 松彦

    第79回応用物理学会 秋季学術講演会  2018.9 

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  • 局所体水分量変化測定のための多孔質マイクロニードルパッチの開発

    熊田 裕希、甲斐 洋行、吉田 昭太郎、西澤 松彦

    第79回応用物理学会 秋季学術講演会  2018.9 

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  • PEDOTとポリウレタンの複合体による伸縮性エレクトロクロミック表示素子

    甲斐 洋行、須田 亘、吉田 昭 太郎、西澤 松彦

    第79回応用物理学会 秋季学術講演会  2018.9 

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  • 分子・電気・光学的透過性を有するハイドロゲル培養皿を用いたヒトiPS細 胞由来心筋細胞の効率的な電気刺激

    李沢 謙介、吉田 昭太郎、西澤 松彦

    第79回応用物理学会 秋季学術講演会  2018.9 

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  • PARYLENE-FILM BIOACTUATOR DRIVEN BY CHICK EMBRYO CARDIOMYOCYTE

    Furuike Kaori, Shima Ai, Yoshida Shotaro, Morimoto Yuya, Takeuchi Shoji

    The Proceedings of the Symposium on Micro-Nano Science and Technology  2018.5 

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    We propose a bioactuator driven by a chick embryo cardiomyocyte for the investigation of cardiomyocytes as alternatives of rodent cardiomyocytes driven actuator. Chick embryo cardiomyocytes are more accessible than rodent cardiomyocytes in terms of cost and ethics. Here, we cultured chick embryo cardiomyocytes on parylene films and observed that the edges of parylene films were autonomously lifted up and down by the contractile force of cardiomyocytes. Moreover, the frequency of flapping increased in response to the addition of isoproterenol, which promotes contraction of cardiac muscles. This bioactuator would reduce the cost of experiments and thus be expected to promote the study of contractile properties of cardiomyocytes.

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  • Microfluidic device for electrofusion of uniformly sized liposomes

    Sugahara Keisuke, Yoshida Shotaro, Takeuchi Shoji

    The Proceedings of the Symposium on Micro-Nano Science and Technology  2018.5 

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    In this paper, we report a microfluidic device for pairing uniformly sized liposomes and fusing the paired liposomes. Our microfluidic device is composed of dynamic micro array for pairing liposomes and three-dimensional electrode for fusing liposomes. Electrodes should be located in proper position of a dynamic micro array for applying sufficient electric field to fuse liposomes. By using low melting point alloy, 3D electrodes were integrated in the dynamic microarray device to fuse the paired liposomes. Two uniformly sized liposomes were successfully paired in the dynamic micro array device and fused by the integrated 3D electrode. We believe that this dynamic micro array device will become a useful microfluidics device for vesicle fusion.

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  • Pairing and electrofusion of cell-sized liposomes by a dielectrophoretic tweezer

    Yoshida Shotaro, Takeuchi Shoji

    The Proceedings of the Symposium on Micro-Nano Science and Technology  2018.5 

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    This manuscript describes selective electrofusion of cell-sized liposomes using microfabricated dielectrophoretic tweezer. We fabricated round-tip dielectrophoretic tweezers by microfabrication techniques. We enabled selective manipulation of liposomes by applying alternate current to the tweezer. As we control the frequency of the current, we attract or repulse the liposomes. Liposomes were selectively manipulated one-by-one and paired by the tweezers. Finally we paired two cell-sized liposomes in a hand-made electrofusion chamber by using the tweezer. The paired liposomes were contact with each other by applying alternate current, then fused by pulses of direct current. We believe our technique will be useful in liposome and cell fusion study for artificial cell research.

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  • 皮膚バリア機能評価のための小型表皮電位測定プローブの開発と応用

    今野創, 阿部結奈, 吉田昭太郎, 西澤松彦

    化学とマイクロ・ナノシステム学会研究会講演要旨集  2018.5 

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  • イオントフォレシスによる高効率な経皮吸収パッチの開発

    草間慎也, 熊田裕希, 豊里涼馬, 甲斐洋行, 吉田昭太郎, 西澤松彦

    化学とマイクロ・ナノシステム学会研究会講演要旨集  2018.5 

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  • ハイドロゲルを基板とする自己変形型カフ電極の開発

    出貝信悟, 李沢謙介, 甲斐洋行, 吉田昭太郎, 西澤松彦

    化学とマイクロ・ナノシステム学会研究会講演要旨集  2018.5 

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  • Development of a dynamic micro array device that enables pairing and fusion of cell-sized liposomes.

    菅原啓亮, 吉田昭太郎, 竹内昌治

    電気学会研究会資料  2018.3 

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  • SELECTIVE PAIRING AND FUSION OF VESICLES USING DIELECTROPHORETIC TWEEZERS

    2018 IEEE MICRO ELECTRO MECHANICAL SYSTEMS (MEMS)  2018.1 

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  • 3D ELECTRODES INTEGRATED IN A DYNAMIC MICRO ARRAY FOR LIPOSOME ELECTROFUSION International conference

    2018 IEEE MICRO ELECTRO MECHANICAL SYSTEMS (MEMS)  2018.1 

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  • リポソームペアリングのためのマイクロ流体デバイス

    菅原啓亮, 吉田昭太郎, 竹内昌治

    化学とマイクロ・ナノシステム学会研究会講演要旨集  2017.10 

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  • リポソームのペアリング及び電気融合のためのマイクロ流体デバイス

    菅原 啓亮, 吉田 昭太郎, 竹内 昌治

    「細胞を創る」研究会10.0  2017.10 

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  • 誘電泳動ピンセットによる細胞サイズリポソームの選択的融合

    吉田 昭太郎, 竹内昌治

    「細胞を創る」研究会10.0  2017.10 

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  • Autonomous bioactuator driven by chicken cardiomyocytes International conference

    Kaori Furuike, Ai Shima, Shotaro Yoshida, Yuya Morimoto, Shoji Takeuchi

    The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017)  2017.10 

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  • Selective pairing and fusion of cell-sized liposomes using round-tip dielectrophoretic tweezer International conference

    Shotaro Yoshida, Shoji Takeuchi

    The 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017)  2017.10 

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  • 非対称貫通孔を有する遠心デバイスによるリポソームの生成

    申東哲, 吉田昭太郎, 森本雄矢, 竹内昌治

    化学とマイクロ・ナノシステム学会研究会講演要旨集  2017.5 

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  • CENTRIFUGE-BASED MEMBRANE EMULSIFICATION TOWARD HIGH-THROUGHPUT GENERATION OF MONODISPERSE LIPOSOMES

    2017 19TH INTERNATIONAL CONFERENCE ON SOLID-STATE SENSORS, ACTUATORS AND MICROSYSTEMS (TRANSDUCERS)  2017 

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  • CELLS SMELL ON A CMOS: A PORTABLE ODORANT DETECTION SYSTEM USING CELL-LADEN COLLAGEN PILLARS

    30TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2017)  2017 

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  • Microplate-based assembly of morphologically controlled neurons for neural circuit construction International conference

    Shotaro Yoshida, Midori Kato-Negishi, Shoji Takeuchi

    LIMMS/CIBIS/CIRMM workshop: Workshop on International research leading to Innovation and new Technology bridging academics and societal demands  2016.12 

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  • 3D assembly of fiber-shaped tissues using micro pillar array devices International conference

    Kaori Furuike, Yuya Morimoto, Shotaro Yoshida, Nobuhito Mori, Shoji Takeuchi

    International Symposium on Micro-Nano Science and Technology 2016  2016.12 

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  • Micropatterning and assembly of primary neurons by mobile microplates International conference

    Shotaro Yoshida, Midori Kato-Negishi, Shoji Takeuchi

    International Symposium on Micro-Nano Science and Technology 2016  2016.12 

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  • L型大腸菌の形態制御のための二光子リソグラフィによる高解像度マイクロチャンバの製作

    吉田 昭太郎, 竹内昌治

    「細胞を創る」研究会9.0  2016.11 

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  • 初代培養神経細胞の形態制御と回路組み立てを行うための単一神経細胞プレート

    吉田昭太郎, 根岸(加藤)みどり, 竹内昌治

    センサ・マイクロマシンと応用システムシンポジウム(CD-ROM)  2016.10 

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  • Spheroids on a CMOS for a portable odorant sensor International conference

    Yusuke Hirata, Shotaro Yoshida, Seiji Tabata, Eunryel Nam, Yuya Morimoto, Shoji Takeuchi

    The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)  2016.10 

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  • 3D assembly of fiber-based tissues combined with micro pillar connectors International conference

    Kaori Furuike, Yuya Morimoto, Shotaro Yoshida, Nobuhito Mori, Shoji Takeuchi

    The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)  2016.10 

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  • Neural puzzle: morphological control and assembly of single primary neurons using mobile microplates International conference

    Shotaro Yoshida, Midori Kato-Negishi, Shoji Takeuchi

    The 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)  2016.10 

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  • CMOSによる細胞センサーのためのスフェロイド蛍光計測

    平田優介, 吉田昭太郎, 田畑誠司, 南垠列, 南垠列, 森本雄矢, 竹内昌治, 竹内昌治

    化学とマイクロ・ナノシステム学会研究会講演要旨集  2016.9 

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  • Single neurons on mobile microplates for neural circuit construction

    吉田昭太郎, 根岸(加藤)みどり, 竹内昌治

    電気学会バイオ・マイクロシステム研究会資料  2016.4 

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  • マイクロデバイスを用いた単一細胞レベルの神経回路構築技術

    吉田 昭太郎, 根岸(加藤) みどり, 竹内 昌治

    第16回東京大学生命科学シンポジウム  2016.4 

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  • CATCH A CELL ON A CMOS: SELECTIVE RETRIEVAL OF SINGLE CELL USING A MICROPLATE TECHNOLOGY PERFORMED ON A CMOS IMAGING SENSOR

    2016 IEEE 29TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS)  2016 

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  • CMOSセンサを用いた一細胞観察システム

    田畑誠司, 森本雄矢, 吉田昭太郎, 竹内昌治

    化学とマイクロ・ナノシステム学会研究会講演要旨集  2015.11 

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  • Stackable Octahedron-based Photoresist Scaffold by Direct Laser Writing for Controlled Three-dimensional Cell Networks International conference

    Florian Larramendy, Shotaro Yoshida, Zoltan Fekete, Daniela Serien, Shoji Takeuchi, Oliver Paul

    The 18th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2015)  2015.6 

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  • A neural cell handling device for assembling cultured neural circuits

    吉田昭太郎, 竹内昌治

    電気学会医用・生体工学研究会資料  2015.3 

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  • PNEUMATIC BALLOON ACTUATOR WITH TUNABLE BENDING POINTS

    2015 28TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2015)  2015 

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  • HIGH-TOPOGRAPHY SURFACE FUNCTIONALIZATION BASED ON PARYLENE-C PEEL-OFF FOR PATTERNED CELL GROWTH

    2015 28TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2015)  2015 

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  • CELL-LADEN HINGED MICROPLATES FOR MEASURING THE CONTRACTILE FORCES OF CARDIOMYOCYTES

    2015 28TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2015)  2015 

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  • Construction of in vitro neural networks by assembling single neural cells using a microfabricated cell-handling device International conference

    Shotaro Yoshida, Shoji Takeuchi

    Society for Neuroscience 2014  2014.11 

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  • 3Dプリンタを用いた3Dダイナミックマイクロアレイデバイスの製作

    LEE Yongseung, 吉田昭太郎, 手島哲彦, 友池史明, 竹内昌治

    センサ・マイクロマシンと応用システムシンポジウム(CD-ROM)  2014.10 

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  • 屈曲点が調整可能な空気圧バルーンアクチュエータ

    ZHENG Lanying, 吉田昭太郎, 森本雄矢, 尾上弘晃, 竹内昌治

    センサ・マイクロマシンと応用システムシンポジウム(CD-ROM)  2014.10 

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  • 単一神経細胞をモジュールとして扱う神経回路アセンブリ

    吉田昭太郎, 竹内昌治

    センサ・マイクロマシンと応用システムシンポジウム(CD-ROM)  2014.10 

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  • マイクロプレートを利用した心筋細胞の収縮特性の計測

    松本周晃, 吉田昭太郎, 森本雄矢, SERIEN Daniela, 森宣仁, 竹内昌治

    化学とマイクロ・ナノシステム学会研究会講演要旨集  2014.10 

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  • 単一神経細胞培養プレートのアセンブリによるin vitro神経回路構築

    吉田昭太郎, 竹内昌治

    化学とマイクロ・ナノシステム学会研究会講演要旨集  2014.10 

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  • Centrifuged-based microfluidics device for trapping single cells in a drop International conference

    Hiromasa Hasegawa, Shotaro Yoshida, Yuya Morimoto, Shoji Takeuchi

    The 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014)  2014.10 

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  • Neural circuit assembly using morphologically controlled single neural cells International conference

    Shotaro Yoshida, Shoji Takeuchi

    The 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014)  2014.10 

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  • Neural circuit assembly at the single cell level by a neural cell-handling device

    Shotaro Yoshida, Shoji Takeuchi

    2014.7 

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  • マイクロプレートによる神経回路アセンブリ

    吉田 昭太郎, 竹内昌治

    バイオアセンブラ第三回若手シンポジウム  2014.7 

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  • 垂直フローリソグラフィー技術における層形状の制御

    幅崎昌平, 幅崎昌平, 吉田昭太郎, 李源哲, 李源哲, 竹内昌治

    化学とマイクロ・ナノシステム学会研究会講演要旨集  2014.5 

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  • 神経ネットワーク構築のための単一神経細胞操作プレート

    吉田 昭太郎, 手島 哲彦, 栗林(繁富) 香織, 竹内 昌治

    数学共働プログラム「生命ダイナミックスの数理とその応用」  2014.1 

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  • ASSEMBLY OF NEURAL CELL-LADEN MICROPLATES ON A MICROFABRICATED BREADBOARD

    2014 IEEE 27TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS)  2014 

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  • 神経回路構成のための単一神経細胞培養プレートの微小電極基板への配置

    吉田昭太郎, 佐藤幸治, 竹内昌治

    化学とマイクロ・ナノシステム学会研究会講演要旨集  2013.12 

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  • 神経細胞の単離培養・形態制御・三次元操作を行うためのマイクロプレート

    吉田昭太郎, 手島哲彦, 栗林(繁富)香織, 竹内昌治

    センサ・マイクロマシンと応用システムシンポジウム(CD-ROM)  2013.11 

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  • 三次元微細造形のための垂直方向の流れを利用したフローリソグラフィー

    幅崎昌平, 吉田昭太郎, LEE Won Chul, LEE Won Chul, 竹内昌治, 竹内昌治

    日本機械学会マイクロ・ナノ工学シンポジウム講演論文集  2013.11 

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  • Fabrication of mountain folds in cell origami by conjoining valley folds

    2013.11 

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  • Three-dimensional fabrication of long and heterogeneous microstructures using vertical continuous flow lithography International conference

    Shohei Habasaki, Shotaro Yoshida, Won Chul Lee, Shoji Takeuchi

    The 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)  2013.10 

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  • Mobile microplates for handling morphologically controlled single neural cells International conference

    Shotaro Yoshida, Tetsuhiko Teshima, Kaori Kuribayashi-Shigetomi, Shoji Takeuchi

    The 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)  2013.10 

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  • 微小プレートによる単一神経細胞の形態制御と三次元操作

    吉田 昭太郎, 手島 哲彦, 栗林(繁富) 香織, 竹内 昌治

    第13回東京大学生命科学シンポジウム  2013.6 

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  • Microfluidically Tunable Lenticular Lens International conference

    Yoshinobu Iimura, Tetsuhiko Teshima, Yun Jung Heo, Yuya Morimoto, Shotaro Yoshida, Hiroaki Onoe, Shoji Takeuchi

    The 17th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2013)  2013.6 

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  • Vertical continuous flow lithography for fabricating long 3D structures International conference

    Shohei Habasaki, Shotaro Yoshida, Won Chul Lee, Shoji Takeuchi

    The 26th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2013)  2013.1 

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  • Sequential micro-assembly of three dimensional biological microstructures from two dimensional cell-laden micro-plates International conference

    Yoshida Shotaro, Sato Koji, Takeuchi Shoji

    FIRST CIRP CONFERENCE ON BIOMANUFACTURING  2013 

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  • DISSOLVABLE MOBILE MICROPLATES FOR HANDLING ADHERENT CELLS

    26TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2013)  2013 

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  • PDMSを用いた液圧駆動型可変焦点レンチキュラーレンズ

    飯村義信, 手島哲彦, HEO YunJung, 森本雄矢, 吉田昭太郎, 尾上弘晃, 竹内昌治

    センサ・マイクロマシンと応用システムシンポジウム(CD-ROM)  2013 

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  • Control of cell actin fiber alignment on paired microplates International conference

    Daniela Serien, Kaori Kuribayashi-Shigetomi, Shotaro Yoshida, Shoji Takeuchi

    Microfluidics for Tissue and Cell Application  2012.11 

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  • Control of self-folding cell-laden microplates by cytoskeleton alignment to fibronectin patterns International conference

    Daniela Serien, Kaori Kuribayashi-Shigetomi, Shotaro Yoshida, Shoji Takeuchi

    The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2012)  2012.10 

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  • Photo-assisted Micro-gluing for Assembling Three Dimensional Microstructures with Living Cells International conference

    Shotaro Yoshida, Koji Sato, Kaori-Kuribayashi Shigetomi, Tetsuhiko Teshima, Shoji Takeuchi

    The 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2012)  2012.10 

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  • 三次元操作可能な生体神経回路モジュール

    吉田 昭太郎, 手島 哲彦, 栗林(繁富) 香織, 竹内 昌治

    日本神経回路学会第22回全国大会  2012.9 

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  • Fibronectin patterning on microplates

    ダニエラ・ゼリーン, 栗林(繁富) 香織, 吉田 昭太郎, 竹内 昌治

    超高速バイオアセンブラ第1回若手研究者シンポジウム  2012.7 

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  • MEMSによる単一神経細胞アセンブリ

    吉田 昭太郎, 竹内昌治

    超高速バイオアセンブラ第1回若手研究者シンポジウム  2012.7 

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  • Generalization characteristics of complex-valued neural networks in dealing with wave-related data

    廣瀬明, 吉田昭太郎

    電子情報通信学会技術研究報告  2012.3 

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    This paper compares the generalization characteristics of complex-valued and real-valued feedforward neural networks in terms of coherence of signals to be dealt with. We assume a task of function approximation such as interpolation of temporal signals. Simulation and real-world experiments demonstrate that complex-valued neural networks with amplitude-phase-type activation function show smaller generalization error than real-valued networks such as bivariate and dual-univariate real-valued neural networks. Based on the results, we discuss how the generalization characteristics are influenced by the coherence of the signals depending on the degree of freedom in the learning and on the circularity in neural dynamics.

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  • Single Neural Cells on Mobile Microplates for Precise Neural Network Assembly International conference

    Shotaro Yoshida, Tetsuhiko Teshima, Kaori Kuribayashi-Shigetomi, Shoji Takeuchi

    The 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2011)  2011.10 

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  • 神経回路を形成するためのマイクロプレートを用いた単一神経細胞操作技術

    吉田 昭太郎, 手島 哲彦, 栗林(繁富) 香織, 竹内 昌治

    第49回日本生物物理学会年会  2011.9 

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  • Generalization characteristics of complex-valued neural networks in dealing with wave-related data

    廣瀬明, 吉田昭太郎

    電子情報通信学会技術研究報告  2011.2 

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    Generalization is one of the most important functions of neural networks. This report compares the generalization characteristics in complex-valued and real-valued neural networks. Assuming that a network deals with wave-related data, we pay attention to the generalization variation dependent on the degree of wave nature. We consider feedforward layered neural networks in complex and real domains. To elucidate the operation difference in the domains, we investigate a simple case where the networks functions for function approximation or filtering of temporal signals. Computer experiments demonstrate that the generalization ability of the complex-valued neural network is better than that of real-valued one in particular when the signal possesses higher degree of wave nature.

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  • Comparison of Complex- and Real-Valued Feedforward Neural Networks in Their Generalization Ability.

    Akira Hirose, Shotaro Yoshida

    Neural Information Processing - 18th International Conference, ICONIP 2011, Shanghai, China, November 13-17, 2011, Proceedings, Part I  2011 

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Awards

  • 若手優秀賞

    2021.3   一般社団法人 化学とマイクロ・ナノシステム学会   有機材料を基盤とする生体親和性ウェアラブル/インプランタブル電子デバイスの開発

    吉田 昭太郎

  • 第25回青葉工学研究奨励賞

    2019.12   一般財団法人 青葉工学振興会   ウェットエレクトロニクス技術による高効率な生体電気刺激・計測システムの開発

    吉田 昭太郎

  • Oral Presentation Award

    2016.10   Institute of Electrical Engineers of Japan  

    Shotaro Yoshida

  • Outstanding Poster Presentation Award

    2013.10   Institute of Electrical Engineers of Japan  

    Shotaro Yoshida

Research Projects

  • ウェアラブル人体通電デバイスのための小型バイオ燃料電池の高出力・高耐久化技術の開発

    2023.2 - 2024.3

    パワーアカデミー  2023年度 パワーアカデミー研究助成  中央大学

    吉田 昭太郎

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  • 加齢時運動機能低下を再現可能な脳-骨格筋モデルの創出

    2021.4 - 2024.3

    日本学術振興会  科学研究費助成事業 基盤研究(B)  分担(代表:東京大学 森本先生)  中央大学

    分担(代表:東京大学 森本雄矢先生)

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  • 新規ゲル基板を用いたフレキシブル全有機電子回路の印刷技術の開発

    2023.1 - 2023.12

    一般財団法人 鷹野学術振興財団  2023年度研究助成  中央大学

    吉田 昭太郎

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  • Single-cell-level Neural Circuit Engineering for BioAI Technology

    Grant number:20H04277  2020.4 - 2023.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)  Chuo University

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    Grant amount: \18070000 ( Direct Cost: \13900000 、 Indirect Cost: \4170000 )

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  • バイオ発電ニードルパッチの開発

    2020.12 - 2022.3

    科学技術振興機構  研究成果最適展開支援プログラム 

    Matsuhiko Nishizawa, Shotaro Yoshida

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    Grant type:Competitive

    Grant amount: \7998900

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  • 表皮電位の低侵襲計測で拓く皮膚イオニクス医工学

    2020.7 - 2022.3

    Japan Society for the Promotion of Science  Grant-in-Aid for Challenging Research (Exploratory) 

    Matsuhiko Nishizawa, Shotaro Yoshida

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    Grant type:Competitive

    Grant amount: \600000 ( Direct Cost: \600000 )

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  • Development of soft electronic device for biosening by integrating organic electronic circuit and hydrogel

    2020.4 - 2021.3

    Tateisi Science and Technology Foundation  Research Grant  Chuo University

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  • 神経プレート技術を用いた一細胞レベル神経回路構築

    2017.4 - 2019.3

    日本学術振興会  科研費・若手研究(B) 

    吉田 昭太郎

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  • 一細胞レベル神経回路構築のための単一神経細胞プレート

    2016.4 - 2017.3

    日本学術振興会  科研費・研究活動スタート支援 

    吉田 昭太郎

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  • 一細胞単位で設計された神経回路を有する三次元人工脳組織の構築

    2013.4 - 2016.3

    日本学術振興会  科研費・特別研究員奨励費 

    吉田 昭太郎

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Allotted class

  • 2023   卒業研究Ⅰ   Department

  • 2023   卒業研究Ⅱ   Department

  • 2023   工学デザイン実習   Department

  • 2023   電子回路1   Department

  • 2023   電子回路2   Department

  • 2023   電気電子情報通信実験   Department

  • 2023   電気電子情報通信工学概論   Department

  • 2023   バイオエレクトロニクス特論   Graduate school

  • 2023   有機エレクトロニクス特論   Graduate school

  • 2023   電気電子情報通信工学論文研修第一   Graduate school

  • 2023   電気電子情報通信工学論文研修第三   Graduate school

  • 2023   電気電子情報通信工学論文研修第二   Graduate school

  • 2023   電気電子情報通信工学論文研修第四   Graduate school

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Teaching Experience

  • bioelectronics

    2021.9 - Now   Institution:Chuo University

  • Organic Electronics

    2021.4 - Now   Institution:Chuo University

  • Electronic Circuits 1

    2020.9 - Now   Institution:Chuo University

  • 電気電子情報通信工学概論

    2020.4 - Now   Institution:中央大学

  • Electronic Circuit 2

    2020.4 - Now   Institution:Chuo University

  • 機械知能・航空実験I

    2018.4 - 2020.3   Institution:東北大学

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Committee Memberships

  • 2021.10 - Now

    電気学会 E部門BMS技術委員会 未来のセンサ調査専門委員会   幹事補佐  

  • 2021.10 - Now

    日本機械学会 マイクロナノ工学部門 未来のセンサに関する研究会   幹事補佐  

  • 2024.1 - 2024.5

    The 19th IEEE International Conference on Nano/Micro Engineered and Molecular Systems   Technical Program Comittee  

  • 2023.4 - 2024.3

    電気学会   電気学会 第40回「センサ・マイクロマシンと応用システム」シンポジウム 論文委員  

  • 2023.4 - 2024.3

    化学とマイクロ・ナノシステム学会   一般社団法人 化学とマイクロ・ナノシステム学会 第48回研究会実行委員  

  • 2023.4 - 2024.3

    日本機械学会   日本機械学会 マイクロ・ナノ工学部門 第14回マイクロ・ナノ工学シンポジウム 実行委員  

  • 2022.4 - 2023.3

    電気学会   電気学会 第39回「センサ・マイクロマシンと応用システム」シンポジウム 論文委員  

  • 2022.4 - 2023.3

    日本機械学会 マイクロ・ナノ工学部門   第13回マイクロ・ナノ工学シンポジウム 実行委員  

  • 2021.10 - 2022.6

    一般社団法人 化学とマイクロ・ナノシステム学会   第45回研究会実行委員  

  • 2021.3 - 2022.4

    日本機械学会   マイクロ・ナノ工学部門 第12回マイクロ・ナノ工学シンポジウム 実行委員  

  • 2021.4 - 2022.3

    電気学会   第38回「センサ・マイクロマシンと応用システム」シンポジウム 論文委員  

  • 2020.3 - 2021.4

    日本機械学会   マイクロ・ナノ工学部門 第11回マイクロ・ナノ工学シンポジウム 実行委員  

  • 2020.4 - 2021.3

    電気学会   第37回「センサ・マイクロマシンと応用システム」シンポジウム 論文委員  

  • 2019.4 - 2020.3

    電気学会   第36回「センサ・マイクロマシンと応用システム」シンポジウム 論文委員  

  • 2020.3 -  

    日本機械学会   マイクロ・ナノ工学部門 第11-13回マイクロ・ナノ工学シンポジウム 実行委員  

  • 2018.4 - 2019.3

    電気学会   第35回「センサ・マイクロマシンと応用システム」シンポジウム 論文委員  

  • 2018.9    

    日本分析化学会   日本分析化学会第67年会会場委員  

  • 2018.4 -  

    電気学会   第35-38回「センサ・マイクロマシンと応用システム」シンポジウム 論文委員  

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