Updated on 2023/04/25

写真a

 
FUJITANI Hirotsugu
 
Organization
Faculty of Science and Engineering Research Associate
Contact information
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大学院生の頃から,地球の窒素循環を駆動させる微生物(硝化菌)を対象に研究しています。培養が難しい硝化菌を獲得するための技術を開発したり,獲得した硝化菌の性状(生理学的な性質やゲノム)を調べてきました。これまでの経験から,個々の微生物が持つ増殖様式や増殖メカニズムは,私たちの予想を超えて多岐に渡っており,神秘的な生命活動が営まれていることが分かってきました。また,同種・異種微生物間相互作用によって生命活動が維持されているケースもあり,微生物といえど一人では生きられない生き物のようです。培養が困難な未培養微生物を獲得することで,科学的にも社会的にも重要かつ面白い発見が可能になります。このフィロソフィを大切にしながら,生命が持つ神秘を追求していきたいです。
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Degree

  • 博士(工学) ( 早稲田大学 )

  • 修士(工学) ( 早稲田大学 )

Education

  • 2013.3
     

    Waseda University   Department of Life Science and Medical Bioscience Doctor Course   doctor course   completed

  • 2009.3
     

    Waseda University   Department of Life Science and Medical Bioscience Master Course   master course   completed

  • 2007.3
     

    Waseda University   Department of Applied Chemistry   graduated

Research History

  • 2020.4 - Now

    Chuo University   理工学部   助教

  • 2019.1 - Now

    Waseda University   Research Organization for Nano and Life Innovation   Guest researcher

  • 2019.1 - 2020.3

    National Institute of Advanced Industrial  Science and Technology (AIST)   Biomedical Research Institute   Postdoctoral Researcher

  • 2018.4 - 2018.12

    Waseda University   Research Organization for Nano & Life Innovation   Assistant professor

  • 2018.1 - 2018.12

    Technical University of Denmark   Department of Environment Engineering   Guest researcher

  • 2016.4 - 2018.3

    Waseda University   Research Organization for Nano & Life Innovation   Junior researcher

  • 2013.4 - 2016.3

    Waseda University   Department of Life Science and Medical Bioscience   Research associate

▼display all

Professional Memberships

  • 環境バイオテクノロジー学会

  • International Society for Microbial Ecology

  • JAPAN SOCIETY ON WATER ENVIRONMENT

  • THE JAPANESE SOCIETY OF MICROBIAL ECOLOGY

  • 日本ゲノム微生物学会

Research Interests

  • nitrite oxidation

  • wastewater

  • cultivation

  • nitrification

  • nitrifier

  • ecophysiology

  • microbial ecology

  • environmental microbes

  • microbial interaction

  • soil

  • isolation

  • nitrogen cycle

  • genome

  • ammonia oxidation

  • heterotrophs

  • autotrophs

Research Areas

  • Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering  / Biofunction/Bioprocess

  • Life Science / Ecology and environment

  • Life Science / Applied microbiology

  • Life Science / Biodiversity and systematics

Papers

  • Characterisation of bacteria representing a novel Nitrosomonas clade: Physiology, genomics and distribution of missing ammonia oxidizer. International journal

    Shuta Kikuchi, Hirotsugu Fujitani, Kento Ishii, Rino Isshiki, Yuji Sekiguchi, Satoshi Tsuneda

    Environmental microbiology reports   2023.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    Members of the genus Nitrosomonas are major ammonia oxidizers that catalyse the first step of nitrification in various ecosystems. To date, six subgenus-level clades have been identified. We have previously isolated novel ammonia oxidizers from an additional clade (unclassified cluster 1) of the genus Nitrosomonas. In this study, we report unique physiological and genomic properties of the strain PY1, compared with representative ammonia-oxidising bacteria (AOB). The apparent half-saturation constant for total ammonia nitrogen and maximum velocity of strain PY1 were 57.9 ± 4.8 μM NH3  + NH4 + and 18.5 ± 1.8 μmol N (mg protein)-1  h-1 , respectively. Phylogenetic analysis based on genomic information revealed that strain PY1 belongs to a novel clade of the Nitrosomonas genus. Although PY1 contained genes to withstand oxidative stress, cell growth of PY1 required catalase to scavenge hydrogen peroxide. Environmental distribution analysis revealed that the novel clade containing PY1-like sequences is predominant in oligotrophic freshwater. Taken together, the strain PY1 had a longer generation time, higher yield and required reactive oxygen species (ROS) scavengers to oxidize ammonia, compared with known AOB. These findings expand our knowledge of the ecophysiology and genomic diversity of ammonia-oxidising Nitrosomonas.

    DOI: 10.1111/1758-2229.13158

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  • Microdroplet-based system for culturing of environmental microorganisms using FNAP-sort Reviewed

    Kanako Saito, Yuri Ota, Dieter M. Tourlousse, Satoko Matsukura, Hirotsugu Fujitani, Masamune Morita, Satoshi Tsuneda, Naohiro Noda

    Sci. Rep.   11 ( 1 )   9506   2021.5

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Nature Research  

    <title>Abstract</title>Droplet microfluidics has emerged as a powerful technology for improving the culturing efficiency of environmental microorganisms. However, its widespread adoption has been limited due to considerable technical challenges, especially related to identification and manipulation of individual growth-positive droplets. Here, we combined microfluidic droplet technology with on-chip “fluorescent nucleic acid probe in droplets for bacterial sorting” (FNAP-sort) for recovery of growth-positive droplets and droplet microdispensing to establish an end-to-end workflow for isolation and culturing of environmental microbes. As a proof-of-concept, we demonstrate the ability of our technique to yield high-purity cultures of rare microorganisms from a representative complex environmental microbiome. As our system employs off-the-shelf commercially available equipment, we believe that it can be readily adopted by others and may thus find widespread use toward culturing the high proportion of as-of-yet uncultured microorganisms in different biomes.

    DOI: 10.1038/s41598-021-88974-2

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    Other Link: http://www.nature.com/articles/s41598-021-88974-2

  • Nitrogen and oxygen isotope signatures of nitrogen compounds during anammox in the laboratory and a wastewater treatment plant Reviewed

    Shotoku Kotajima, Keisuke Koba, Daisuke Ikeda, Akihiko Terada, Kazuichi Isaka, Kazuya Nishina, Yuuya Kimura, Akiko Makabe, Midori Yano, Hirotsugu Fujitani, Norisuke Ushiki, Satoshi Tsuneda, Muneoki Yoh

    Microbes Environ.   35 ( 4 )   2020.11

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    Isotopic fractionation factors against 15N and 18O during anammox (anaerobic ammonia oxidization by nitrite) are critical for evaluating the importance of this process in natural environments. We performed batch incubation experiments with an anammox-dominated biomass to investigate nitrogen (N) and oxygen (O) isotopic fractionation factors during anammox and also examined apparent isotope fractionation factors during anammox in an actual wastewater treatment plant. We conducted one incubation experiment with high δ18O of water to investigate the effects of water δ18O. The N isotopic fractionation factors estimated from incubation experiments and the wastewater treatment plant were similar to previous values. We also found that the N isotopic effect (15εNXR of -77.8 to -65.9‰ and 15ΔNXR of -31.3 to -30.4‰) and possibly O isotopic effect (18εNXR of -20.6‰) for anaerobic nitrite oxidation to nitrate were inverse. We applied the estimated isotopic fractionation factors to the ordinary differential equation model to clarify whether anammox induces deviations in the δ18O vs δ15N of nitrate from a linear trajectory of 1, similar to heterotrophic denitrification. Although this deviation has been attributed to nitrite oxidation, the O isotopic fractionation factor for anammox is crucial for obtaining a more detailed understanding of the mechanisms controlling this deviation. In our model, anammox induced the trajectory of the δ18O vs δ15N of nitrate during denitrification to less than one, which strongly indicates that this deviation is evidence of nitrite oxidation by anammox under denitrifying conditions.

    DOI: 10.1264/jsme2.ME20031

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  • Enrichment of comammox and nitrite-oxidizing Nitrospira from acidic soil Reviewed

    Yu Takahashi, Hirotsugu Fujitani, Yuhei Hirano, Kanako Tago, Yong Wang, Masahito Hayatsu, Satoshi Tsuneda

    Front. Microbiol.   11 ( 1737 )   1737   2020.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Frontiers  

    DOI: 10.3389/fmicb.2020.01737

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  • MazF endoribonucleolytic toxin conserved in Nitrospira specifically cleaves the AACU, AACG, and AAUU motifs Reviewed

    Rie Aoi, Tatsuki Miyamoto, Akiko Yokota, Yuri Ota, Hirotsugu Fujitani, Satoshi Tsuneda, Naohiro Noda

    Toxins   12 ( 287 )   2020.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI  

    DOI: 10.3390/toxins12050287

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  • Physiological and genomic characterization of a new “Candidatus Nitrotoga” isolate Reviewed

    Kento Ishii, Hirotsugu Fujitani, Yuji Sekiguchi, Satoshi Tsuneda

    Environmental Microbiology   22 ( 6 )   2365 - 2382   2020.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley-Blackwell  

    DOI: 10.1111/1462-2920.15015

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  • Transcriptome analysis of the ammonia-oxidizing bacterium Nitrosomonas mobilis Ms1 reveals division of labor between aggregates and free-living cells Reviewed

    Rino Isshiki, Hirotsugu Fujitani, Satoshi Tsuneda

    Microbes and Environments   35 ( 2 )   2020.2

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Japanese Society of Microbial Ecology  

    Bacteria change their metabolic states to increase survival by forming aggregates. Ammonia-oxidizing bacteria also form aggregates in response to environmental stresses. Nitrosomonas mobilis, an ammonia-oxidizing bacterium with high stress tolerance, often forms aggregates mainly in wastewater treatment systems. Despite the high frequency of aggregate formation by N. mobilis, its relationship with survival currently remains unclear. In the present study, aggregates were formed in the late stage of culture with the accumulation of nitrite as a growth inhibitor. To clarify the significance of aggregate formation in N. mobilis Ms1, a transcriptome analysis was performed. Comparisons of the early and late stages of culture revealed that the expression of stress response genes (chaperones and proteases) increased in the early stage. Aggregate formation may lead to stress avoidance because stress response genes were not up-regulated in the late stage of culture during which aggregates formed. Furthermore, comparisons of free-living cells with aggregates in the early stage of culture showed differences in gene expression related to biosynthesis (ATP synthase and ribosomal proteins) and motility and adhesion (flagella, pilus, and chemotaxis). Biosynthesis genes for growth were up-regulated in free-living cells, while motility and adhesion genes for adaptation were up-regulated in aggregates. These results indicate that N. mobilis Ms1 cells adapt to an unfavorable environment and grow through the division of labor between aggregates and free-living cells.

    DOI: 10.1264/jsme2.ME19148

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  • Successful enrichment of low-abundant comammox Nitrospira from nitrifying granules under ammonia-limited conditions Reviewed

    Hirotsugu Fujitani†, Manami Nomachi†, Yu Takahashi†, Yoshiaki Hasebe, Masahiro Eguchi, Satoshi Tsuneda

    FEMS Microbiology Letters   367 ( 1 )   fna025   2020.2

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    DOI: 10.1093/femsle/fnaa025

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  • Draft genome sequence of Acidovorax sp. strain NB1 isolated from a nitrite-oxidizing enrichment culture Reviewed

    Hiroto Ide, Kento Ishii, Hirotsugu Fujitani, Satoshi Tsuneda

    Microbiology Resource Announcements   8 ( 33 )   e00547-19   2019.8

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  • Seabird-affected taluses are denitrification hotspots and potential N2O emitters in the High Arctic Reviewed

    Hayashi, Kentaro, Tanabe, Yukiko, Ono, Keisuke, Loonen, Maarten J. J. E., Asano, Maki, Fujitani, Hirotsugu, Tokida, Takeshi, Uchida, Masaki, Hayatsu, Masahito

    SCIENTIFIC REPORTS   8 ( 17261 )   2018.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:NATURE PUBLISHING GROUP  

    In High Arctic tundra ecosystems, seabird colonies create nitrogen cycling hotspots because of bird-derived labile organic matter. However, knowledge about the nitrogen cycle in such ornithocoprophilous tundra is limited. Here, we determined denitrification potentials and in-situ nitrous oxide (N2O) emissions of surface soils on plant-covered taluses under piscivorous seabird cliffs at two sites (BL and ST) near Ny-Alesund, Svalbard, in the European High Arctic. Talus soils at both locations had very high denitrification potentials at 10 degrees C (2.62-4.88 mg N kg(-1) dry soil h(-1)), near the mean daily maximum air temperature in July in Ny-Alesund, with positive temperature responses at 20 degrees C (Q10 values, 1.6-2.3). The talus soils contained abundant denitrification genes, suggesting that they are denitrification hotspots. However, high in-situ N2O emissions, indicating the presence of both active aerobic nitrification and anaerobic denitrification, were observed only at BL (max. 16.6 mu g N m(-2) h(-1)). Rapid nitrogen turnover at BL was supported by lower carbon-to-nitrogen ratios, higher nitrate content, and higher delta N-15 values in the soils at BL compared with those at ST. These are attributed to the 30-fold larger seabird density at BL than at ST, providing the larger organic matter input.

    DOI: 10.1038/s41598-018-35669-w

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  • Genomic analysis of two phylogenetically distinct Nitrospira species reveals their genomic plasticity and functional diversity Reviewed

    Norisuke Ushiki, Hirotsugu Fujitani, Yu Shimada, Tomohiro Morohoshi, Yuji Sekiguchi, Satoshi Tsuneda

    Frontiers in Microbiology   8 ( 2637 )   2018.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Frontiers Media S.A.  

    The genus Nitrospira represents a dominant group of nitrite-oxidizing bacteria in natural and engineered ecosystems. This genus is phylogenetically divided into six lineages, for which vast phylogenetic and functional diversity has been revealed by recent molecular ecophysiological analyses. However, the genetic basis underlying these phenotypic differences remains largely unknown because of the lack of genome sequences representing their diversity. To gain a more comprehensive understanding of Nitrospira, we performed genomic comparisons between two Nitrospira strains (ND1 and NJ1 belonging to lineages I and II, respectively) previously isolated from activated sludge. In addition, the genomes of these strains were systematically compared with previously reported six Nitrospira genomes to reveal their similarity and presence/absence of several functional genes/operons. Comparisons of Nitrospira genomes indicated that their genomic diversity reflects phenotypic differences and versatile nitrogen metabolisms. Although most genes involved in key metabolic pathways were conserved between strains ND1 and NJ1, assimilatory nitrite reduction pathways of the two Nitrospira strains were different. In addition, the genomes of both strains contain a phylogenetically different urease locus and we confirmed their ureolytic activity. During gene annotation of strain NJ1, we found a gene cluster encoding a quorum-sensing system. From the enriched supernatant of strain NJ1, we successfully identified seven types of acyl-homoserine lactones with a range of C10-C14. In addition, the genome of strain NJ1 lacks genes relevant to flagella and the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas (CRISPR-associated genes) systems, whereas most nitrifying bacteria including strain ND1 possess these genomic elements. These findings enhance our understanding of genomic plasticity and functional diversity among members of the genus Nitrospira.

    DOI: 10.3389/fmicb.2017.02637

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  • Enrichment and Physiological Characterization of a Cold-Adapted Nitrite-Oxidizing Nitrotoga sp from an Eelgrass Sediment Reviewed

    Kento Ishii, Hirotsugu Fujitani, Kentaro Soh, Tatsunori Nakagawa, Reiji Takahashi, Satoshi Tsuneda

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   83 ( 14 )   e00549-17   2017.7

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER SOC MICROBIOLOGY  

    Nitrite-oxidizing bacteria (NOB) are responsible for the second step of nitrification in natural and engineered ecosystems. The recently discovered genus Nitrotoga belongs to the Betaproteobacteria and potentially has high environmental importance. Although environmental clones affiliated with Nitrotoga are widely distributed, the limited number of cultivated Nitrotoga spp. results in a poor understanding of their ecophysiological features. In this study, we successfully enriched the nonmarine cold-adapted Nitrotoga sp. strain AM1 from coastal sand in an eelgrass zone and investigated its physiological characteristics. Multistep-enrichment approaches led to an increase in the abundance of AM1 to approximately 80% of the total bacterial population. AM1 was the only detectable NOB in the bacterial community. The 16S rRNA gene sequence of AM1 was 99.6% identical to that of "Candidatus Nitrotoga arctica," which was enriched from permafrost-affected soil. The highest nitrogen oxidation rate of AM1 was observed at 16 degrees C. The half-saturation constant (K-m) and the generation time were determined to be 25 mu M NO2- and 54 h, respectively. The nitrite oxidation rate of AM1 was stimulated at concentrations of &lt;30 mM NH4Cl but completely inhibited at 50 mM NH4Cl. AM1 can grow well under specific environmental conditions, such as low temperature and in the presence of a relatively high concentration of free ammonia. These results help improve our comprehension of the functional importance of Nitrotoga.
    IMPORTANCE Nitrite-oxidizing bacteria (NOB) are key players in the second step of nitrification, which is an important process of the nitrogen cycle. Recent studies have suggested that the organisms of the novel NOB genus Nitrotoga were widely distributed and played a functional role in natural and engineered ecosystems. However, only a few Nitrotoga enrichments have been obtained, and little is known about their ecology and physiology. In this study, we successfully enriched a Nitrotoga sp. from sand in a shallow coastal marine ecosystem and undertook a physiological characterization. The laboratory experiments showed that the Nitrotoga enrichment culture could adapt not only to low temperature but also to relatively high concentrations of free ammonia. The determination of as-yet-unknown unique characteristics of Nitrotoga contributes to the improvement of our insights into the microbiology of nitrification.

    DOI: 10.1128/AEM.00549-17

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  • Nitrite oxidation kinetics of two Nitrospira strains: The quest for competition and ecological niche differentiation Reviewed

    Norisuke Ushiki, Masaru Jinno, Hirotsugu Fujitani, Toshikazu Suenaga, Akihiko Terada, Satoshi Tsuneda

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   123 ( 5 )   581 - 589   2017.5

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SOC BIOSCIENCE BIOENGINEERING JAPAN  

    Nitrite oxidation is an aerobic process of the nitrogen cycle in natural ecosystems, and is performed by nitrite-oxidizing bacteria (NOB). Also, nitrite oxidation is a rate-limiting step of nitrogen removal in wastewater treatment plants (WWTPs). Although Nitrospira is known as dominant NOB in WWTPs, information on their physiological properties and kinetic parameters is limited. Here, we report the kinetic parameters and inhibition of nitrite oxidation by free ammonia in pure cultures of Nitrospira sp. strain ND1 and Nitrospira japonica strain NJ1, which were previously isolated from activated sludge in a WWTP. The maximum nitrite uptake rate (V-max_NO2) and the half-saturation constant for nitrite uptake (K-m_NO2) of strains ND1 and NJ1 were 45 +/- 7 and 31 +/- 5 (mu mol NO2-/mg protein/h), and 6 +/- 1 and 10 +/- 2 (mu M NO2-), respectively. The V-max_NO2 and K-m_NO2 of two strains indicated that they adapt to low-nitrite-concentration environments like activated sludge. The half-saturation constants for oxygen uptake (K-m_O2) of the two strains were 4.0 +/- 2.5 and 2.6 +/- 1.1 (mu M O-2), respectively. The K-m_O2 values of the two strains were lower than those of other NOB, suggesting that Nitrospira in activated sludge could oxidize nitrite in the hypoxic environments often found in the interiors of biofilms and flocs. The inhibition thresholds of the two strains by free ammonia were 0.85 and 4.3 (mg-NH3 I-1), respectively. Comparing the physiological properties of the two strains, we suggest that tolerance for free ammonia determines competition and partitioning into ecological niches among Nitrospira populations. (C) 2017, The Society for Biotechnology, Japan. All rights reserved.

    DOI: 10.1016/j.jbiosc.2016.12.016

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  • Detection and Diversity of the Nitrite Oxidoreductase Alpha Subunit (nxrA) Gene of Nitrospina in Marine Sediments Reviewed

    Sundas Rani, Hyeon-Woo Koh, Sung-Keun Rhee, Hirotsugu Fujitani, Soo-Je Park

    MICROBIAL ECOLOGY   73 ( 1 )   111 - 122   2017.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER  

    Nitrite-oxidizing bacteria (NOB) are chemolithoautotrophs that catalyze the oxidation of nitrite to nitrate, which is the second step of aerobic nitrification. In marine ecosystems, Nitrospina is assumed to be a major contributor to nitrification. To date, two strains of Nitrospina have been isolated from marine environments. Despite their ecological relevance, their ecophysiology and environmental distribution are understudied owing to fastidious cultivation techniques and the lack of a sufficient functional gene marker. To estimate the abundance, diversity, and distribution of Nitrospina in various marine sediments, we used nxrA, which encodes the alpha subunit of nitrite oxidoreductase, as a functional and phylogenetic marker. We observed that Nitrospina diversity in polar sediments was significantly lower than that of non-polar samples. Moreover, nxrA-like sequences revealed an unexpected diversity of Nitrospina, with approximately 41,000 different sequences based on a 95% similarity cutoff from six marine sediments. We detected nxrA gene copy numbers of up to 3.57 x 10(4) per gram of marine sediment sample. The results of this study provide insight into the distribution and diversity of Nitrospina, which is fundamentally important for understanding their contribution to the nitrogen cycle in marine sediments.

    DOI: 10.1007/s00248-016-0897-3

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  • A rapid collection of yet unknown ammonia oxidizers in pure culture from activated sludge Reviewed

    Takuma Abe, Norisuke Ushiki, Hirotsugu Fujitani, Satoshi Tsuneda

    WATER RESEARCH   108   169 - 178   2017.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PERGAMON-ELSEVIER SCIENCE LTD  

    Nitrification is an important reaction in the biological nitrogen removal process in wastewater treatment plants (WWTPs). As ammonia-oxidizing microbes are slow-growing and sensitive to environmental factors such as free ammonia, pure strains are hard to obtain, preventing our understanding of their physiological characteristics. To conquer this hurdle, we report a high-throughput isolation technique based on scattering signatures, which exploits the tendency of many ammonia-oxidizing bacteria (AOB) to form microcolonies in activated sludge. The AOB microcolonies were directly sorted from the activated sludge without long incubation and enrichment bias, and were sequentially inoculated into 96-well microtiter plates containing growth medium. Phylogenetic analysis of the pure strains isolated in this study revealed a deeply branching and unrecognized lineage and diversity within the genus Nitrosomonas, beyond our expectation. (C) 2016 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.watres.2016.10.070

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  • Ecophysiology and Comparative Genomics of Nitrosomonas mobilis Ms1 Isolated from Autotrophic Nitrifying Granules of Wastewater Treatment Bioreactor Reviewed

    Soe Myat Thandar, Norisuke Ushiki, Hirotsugu Fujitani, Yuji Sekiguchi, Satoshi Tsuneda

    FRONTIERS IN MICROBIOLOGY   7 ( 1869 )   2016.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:FRONTIERS MEDIA SA  

    Ammonia-oxidizing bacteria (AOB), which oxidize ammonia to nitrite in the first step of nitrification, play an important role in biological wastewater treatment systems. Nitrosomonas mobilis is an important and dominant AOB in various wastewater treatment systems. However, the detailed physiological and genomic properties of N. mobilis have not been thoroughly investigated because of limited success isolating pure cultures. This study investigated the key physiological characteristics of N. mobilis Ms1, which was previously isolated into pure culture from the nitrifying granules of wastewater treatment bioreactor. The pure culture of N. mobilis Ms1 was cultivated in liquid mineral medium with 30 mg-NL-1 (2.14 mM) of ammonium at room temperature under dark conditions. The optimum growth of N. mobilis Ms1 occurred at 27 degrees C and pH 8, with a maximum growth rate of 0.05-0.07 h(-1), which corresponded to a generation time of 10-14 h. The half saturation constant for ammonium uptake rate and the maximum ammonium uptake rate of N. mobilis Ms1 were 30.70 +/- 0.51 mu M NH4+ and 0.01 +/- 0.002 pmol NH4+ cells(-1) h(-1), respectively. N. mobilis Ms1 had higher ammonia oxidation activity than N. europaea in this study. The oxygen uptake activity kinetics of N. mobilis Ms1 were K-m(O2) = 21.74 +/- 4.01 it mu M O-2 and V-max(O2) = 0.06 +/- 0.02 pmol O-2 cells(-1) h(-1). Ms1 grew well at ammonium and NaCl concentrations of up to 100 and 500 mM, respectively. The nitrite tolerance of N. mobilis Ms1 was extremely high (up to 300 mM) compared to AOB previously isolated from activated sludge and wastewater treatment plants. The average nucleotide identity between the genomes of N. mobilis Ms1 and other Nitrosomonas species indicated that N. mobilis Ms1 was distantly related to other Nitrosomonas species. The organization of the genes encoding protein inventory involved in ammonia oxidation and nitrifier denitrification processes were different from other Nitrosomonas species. The current study provides a needed physiological and genomic characterization of N. mobilis-like bacteria and a better understanding of their ecophysiological properties, enabling comparison of these bacteria with other AOB in wastewater treatment systems and natural ecosystems.

    DOI: 10.3389/fmicb.2016.01869

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  • Physical enrichment of uncultured Accumulibacter and Nitrospira from activated sludge by unlabeled cell sorting technique Reviewed

    Kana Irie, Hirotsugu Fujitani, Satoshi Tsuneda

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   122 ( 4 )   475 - 481   2016.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SOC BIOSCIENCE BIOENGINEERING JAPAN  

    It is important to understand the ecology and physiology of microbes in activated sludge of wastewater treatment plants. Recently, molecular based approaches such as 16S rRNA genes and environmental genomics have illuminated black boxes in nutrient removal process and expanded our knowledge. However, most microbes responsible for the removal of phosphate and nitrogen such as Accumulibacter and Nitrospira remain uncultured. This is because optimum methodologies to concentrate these uncultured microbes and to obtain pure cultures have not been established. Here, we report a novel approach for physical enrichment of uncultured Accumulibacter and Nitrospira from microbial communities in activated sludge by a cell sorting system. Two scattering signatures representing forward scatter and side scatter of this system allowed morphological characterization of microbial particles in activated sludge. The distribution and size of microbial particles consisting of single cells, microcolonies, and aggregates depended on the levels of scattering signatures. Next generation sequencer and principal component analysis revealed each microbial population fractionated according to the levels of scattering signatures, resulting that uncultured Accumulibacter and Nitrospira could be sorted as single cells or microcolonies. Finally, quantitative fluorescence in situ hybridization analysis determined optimum fractions to collect sufficiently these target microbes from activated sludge. Consequently, this method would be very useful as an enrichment technique prior to isolation, genomic analysis, and physiological investigation of uncultured bacteria. (C) 2016, The Society for Biotechnology, Japan. All rights reserved.

    DOI: 10.1016/j.jbiosc.2016.03.020

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  • Selective isolation of ammonia-oxidizing bacteria from autotrophic nitrifying granules by applying cell-sorting and sub-culturing of microcolonies Reviewed

    Hirotsugu Fujitani, Asami Kumagai, Norisuke Ushiki, Kengo Momiuchi, Satoshi Tsuneda

    FRONTIERS IN MICROBIOLOGY   6 ( 1159 )   2015.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:FRONTIERS MEDIA SA  

    Nitrification is a key process in the biogeochemical nitrogen cycle and biological wastewater treatment that consists of two stepwise reactions, ammonia oxidation by ammonia-oxidizing bacteria (AOB) or archaea followed by nitrite oxidation by nitrite-oxidizing bacteria. One of the representatives of the AOB group is Nitrosomonas mobilis species. Although a few pure strains of this species have been isolated so far, approaches to their preservation in pure culture have not been established. Here, we report isolation of novel members of the N. mobilis species from autotrophic nitrifying granules used for ammonia-rich wastewater treatment. We developed an isolation method focusing on microcolonies formation of nitrifying bacteria. Two kinds of distinctive light scattering signatures in a cell-sorting system enabled to separate microcolonies from single cells and heterogeneous aggregates within granule samples. Inoculation of a pure microcolony into 96-well microtiter plates led to successful subculturing and increased probability of isolation. Obtained strain Ms1 is cultivated in the liquid culture with relatively high ammonia or nitrite concentration, not extremely slow growing. Considering environmental clones that were closely related to N. mobilis and detected in various environments, the availability of this novel strain would facilitate to reveal this member's ecophysiology in a variety of habitats.

    DOI: 10.3389/fmicb.2015.01159

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  • A novel cultivation and isolation strategy for uncultured nitrite-oxidizing bacteria of the genus Nitrospira Invited

    Hirotsugu Fujitani, Satoshi Tsuneda

    Journal of Environmental Biotechnology   14 ( 2 )   99 - 103   2015.3

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  • Isolation of sublineage I Nitrospira by a novel cultivation strategy Reviewed

    Hirotsugu Fujitani, Norisuke Ushiki, Satoshi Tsuneda, Yoshiteru Aoi

    ENVIRONMENTAL MICROBIOLOGY   16 ( 10 )   3030 - 3040   2014.10

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    Nitrification is an important process in the biogeochemical nitrogen cycle and is widely exploited in biological wastewater treatment. Recently, Nitrospira has been recognized as the numerically dominant nitrite-oxidizing bacterial genus and is primarily responsible for the second step of aerobic nitrification. Nevertheless, the physiological properties of Nitrospira remain poorly understood because the organisms are difficult to isolate and culture. Here, we report a novel cultivation strategy for obtaining members of the Nitrospira sublineage I in pure culture. The method combines: (i) selective enrichment of Nitrospira using a continuous feeding reactor and (ii) purification followed by sub-cultivation via a cell sorting system by focusing on the unique characteristics of Nitrospira forming spherical micro-colonies. This strategy is potentially applicable to other uncultured or unisolated Nitrospira and could accelerate the physiological and biochemical understandings of this important group of organisms.

    DOI: 10.1111/1462-2920.12248

    Web of Science

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  • Isolation of Nitrospira belonging to Sublineage II from a Wastewater Treatment Plant Reviewed

    Norisuke Ushiki, Hirotsugu Fujitani, Yoshiteru Aoi, Satoshi Tsuneda

    MICROBES AND ENVIRONMENTS   28 ( 3 )   346 - 353   2013.9

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    Nitrite oxidation is a key step in nitrogen removal in biological wastewater treatment plants. Recently, two phylogenetically different Nitrospira (sublineages I and II) have been recognized as the numerically dominant nitrite-oxidizing bacteria in wastewater treatment plants. However, Nitrospira sublineage II inhabiting activated sludge was not isolated and its detailed properties were unclear. In this study, we developed a new method for the isolation of Nitrospira forming micro-colonies using a cell sorter. We obtained a novel pure strain "Nitrospira japonica" from the activated sludge. Subsequently, phylogenetic and physiological analyses revealed that Nitrospira japonica belongs to sublineage II and grew in medium containing formate. This method has the potential to isolate other uncultured microorganisms forming micro-colonies.

    DOI: 10.1264/jsme2.ME13042

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  • Selective enrichment of two different types of Nitrospira-like nitrite-oxidizing bacteria from a wastewater treatment plant Reviewed

    Hirotsugu Fujitani, Yoshiteru Aoi, Satoshi Tsuneda

    Microbes and Environments   28 ( 2 )   236 - 243   2013.6

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    Nitrification is an important step in nitrogen removal in biological wastewater treatment processes. Recently, Nitrospira have been recognized as the numerically dominant nitrite-oxidizing bacterial genus primarily responsible for the second step of aerobic nitrification
    however, Nitrospira usually resist cultivation under laboratory conditions and only one species enriched from activated sludge has been described. In this study, a novel enrichment method for Nitrospira was successfully developed using continuous feeding bioreactors. By controlling nitrite concentrations strictly in the bioreactor at low levels below 10 mg-N L-1, coexisting members of sublineages I and II of the genus Nitrospira were enriched selectively. The maximum ratios of sublineages I and II to total microbial cells achieved 88.3% and 53.8%, respectively. This enrichment method is potentially applicable to other uncultured Nitrospira.

    DOI: 10.1264/jsme2.ME12209

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Presentations

  • 未培養硝化菌の分離戦略と生理生態 Invited

    藤谷拓嗣

    環境バイオテクノロジー学会2021年度大会  2021.9 

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  • 地球の窒素循環を駆動する未培養微生物の実態に迫る Invited

    藤谷拓嗣

    第15回細菌学若手コロッセウム  2021.8 

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  • Comparative physiology and genome of ammonia-oxidizing Nitrosospira isolated from terrestrial and freshwater environments International conference

    Emi Nissato, Hirotsugu Fujitani, Keisuke Sakai, Hiroshi Ishikawa, Yuichi Suwa

    7th International Conference on Nitrification  2021.7 

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  • 新規な分離培養技術が紐解く未知微生物:硝化菌の生き様とは?

    SATテクノロジーショーケース2020  2020.1 

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  • Genomic and physiological characteristics of novel nitrite-oxidizing Nitrospira strain isolated from a drinking water treatment plant International conference

    Hirotsugu Fujitani, Kengo Momiuchi, Kento Ishii, Masaru Jinno, Norisuke Ushiki, Yuji Sekiguchi, Satoshi Tsuneda

    7th International Conference Microbial Ecology and Water Engineering  2019.11 

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  • Enrichment of comammox Nitrospira from nitrifying granules by using fixed-bed continuous feeding bioreactors International conference

    Hirotsugu Fujitani, Manami Nomachi, Yu Takahashi, Yoshiaki Hasebe, Masahiro Eguchi, Satoshi Tsuneda

    7th International Conference Microbial Ecology and Water Engineering  2019.11 

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  • Enrichment of comammox Nitrospira from nitrifying granules by using fixed-bed continuous feeding bioreactors International conference

    Hirotsugu Fujitani, Manami Nomachi, Yu Takahashi, Yoshiaki Hasebe, Masahiro Eguchi, Satoshi Tsuneda

    6th International Conference on Nitrification  2019.10 

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  • Characterization of a bacterium representing a previously unrecognized clade of the Nitrosomonas group; physiology, genomics, and distribution of a missing ammonia-oxidizer International conference

    Hirotsugu Fujitani, Shuta Kikuchi, Kento Ishii, Yuji Sekiguchi, Satoshi Tsuneda

    6th International Conference on Nitrification  2019.10 

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  • 培養技術とゲノム解析で紐解く硝化菌の生理生態 Invited

    藤谷 拓嗣

    第22回日本水環境学会シンポジウム  2019.9 

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  • Cultivation of a bacterium representing a previously unrecognized clade of the Nitrosomonas group; physiology, genomics, and distribution of a missing ammonia-oxidizer International conference

    Hirotsugu Fujitani, Shuta Kikuchi, Kento Ishii, Yuji Sekiguchi, Satoshi Tsuneda

    8th Congress of European Microbiologists  2019.7 

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  • 未培養硝化菌を対象とした新規分離培養技術の開発

    藤谷拓嗣, 常田聡

    環境バイオテクノロジー学会2019年度大会  2019.6 

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  • 見過ごされたアンモニア酸化細菌:Nitrosomonas属の新規な生理学,ゲノミクス,環境分布

    藤谷拓嗣, 菊池脩太, 石井拳人, 関口勇地, 常田聡

    第13回日本ゲノム微生物学会年会  2019.3 

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  • Genomic and physiological characteristics of novel nitrite-oxidizing Nitrospira isolated from a drinking water treatment plant International conference

    Hirotsugu Fujitani, Kengo Momiuchi, Kento Ishii, Masaru Jinno, Norisuke Ushiki, Yuji Sekiguchi, Satoshi Tsuneda

    17th International society for microbial ecology  2018.8 

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  • Comparative genomics of phylogenetically distinct two Nitrospira strains isolated from activated sludge International conference

    Norisuke Ushiki, Hirotsugu Fujitani, Yu Shimada, Tomohiro Morohoshi, Yuji Sekiguchi, Satoshi Tsuneda

    5th International Conference on Nitrification  2017.7 

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  • Ammonia concentration determines ecological niche differentiation of Nitrospira as nitrite-oxidizers in activated sludge International conference

    Hirotsugu Fujitani, Masaru Jinno, Norisuke Ushiki, Toshikazu Suenaga, Akihiko Terada, Satoshi Tsuneda

    6th International Conference Microbial Ecology and Water Engineering  2016.9 

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  • Direct isolation of microcolonies from activated sludge opens the window of unrecognized genus Nitrosomonas as ammonia oxidizers International conference

    Hirotsugu Fujitani, Takuma Abe, Norisuke Ushiki, Satoshi Tsuneda

    16th International society for microbial ecology  2016.8 

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  • 新規な分離培養技術によって獲得された硝化細菌の生理・ゲノム特性

    藤谷拓嗣, 牛木章友, 神野 大, 安部拓磨, 常田 聡

    環境バイオテクノロジー学会2016年度大会  2016.6 

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  • Physiological and genomic characteristics of uncultured nitrifiers illuminated by novel cultivation and isolation strategies Invited

    Hirotsugu Fujitani, Norisuke Ushiki, Satoshi Tsuneda

    2016.3 

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  • Isolation, physiology, and genomics of novel nitrifiers Invited International conference

    Hirotsugu Fujitani, Satoshi Tsuneda

    31th Japanese Society Microbial Ecology Annual Meeting and 7th Japan-Taiwan-Korea Symposium on Microbial Ecology  2015.10 

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  • Isolation and successful subculture of Nitrosomonas mobilis lineage: Recovery of nature's lost treasure since 1970's International conference

    Hirotsugu Fujitani, Asami Kumagai, Norisuke Ushiki, Kengo Momiuchi, Satoshi Tsuneda

    4th International Conference on Nitrification  2015.6 

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  • 亜硝酸酸化細菌Nitrospiraを標的とした新規分離培養手法と他の難培養性微生物への適用

    藤谷拓嗣, 籾内研吾, 牛木章友, 入江香菜, 熊谷麻美, 安部拓磨, 神野 大, 青井議輝, 常田 聡

    第29回日本微生物生態学会  2013.11 

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  • 難培養性亜硝酸酸化細菌Nitrospiraを標的とした革新的分離培養戦略

    藤谷拓嗣, 牛木章友, 籾内研吾, 青井議輝, 常田 聡

    第16回日本水環境学会シンポジウム  2013.11 

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  • Selective inoculation of microcolonies materializes cultivation and isolation of previously uncultured nitrifying bacteria International conference

    Hirotsugu Fujitani, Norisuke Ushiki, Kengo momiuchi, Asami Kumagai, Takuma Abe, Satoshi Tsuneda

    The 5th Taiwan-Korea-Japan International Symposium on Microbial Ecology  2013.10 

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  • An innovative cultivation and isolation strategy for uncultured nitrifying bacteria International conference

    Hirotsugu Fujitani, Norisuke Ushiki, Kengo Momiuchi, Yoshiteru Aoi, Satoshi Tsuneda

    3rd International conference on nitrification  2013.9 

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  • An innovative cultivation and isolation strategy for uncultured nitrifying bacteria International conference

    Hirotsugu Fujitani, Norisuke Ushiki, Kengo Momiuchi, Yoshiteru Aoi, Satoshi Tsuneda

    Early career scientist workshop ICoN3  2013.9 

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  • An innovative cultivation and isolation strategy for uncultured nitrite-oxidizing bacteria of the genus Nitrospira International conference

    Hirotsugu Fujitani, Norisuke Ushiki, Kengo Momiuchi, Yoshiteru Aoi, Satoshi Tsuneda

    5th International conference Microbial Ecology and Water Engineering  2013.7 

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  • An innovative cultivation and isolation strategy for uncultured nitrifying bacteria International conference

    Hirotsugu Fujitani, Norisuke Ushiki, Kengo momiuchi, Yoshiteru Aoi, Satoshi Tsuneda

    CER International workshop on Biogeochemical cycling and Microbial Ecology for Young Scientists  2013.3 

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  • Isolation of previously uncultured Nitrospira unveils the physiology of nitrite-oxidizing bacteria in wastewater treatment process

    2012.9 

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  • Novel method for uncultured nitrifying bacteria Invited International conference

    Hirotsugu Fujitani, Norisuke Ushiki, Satoshi Tsuneda, Yoshiteru Aoi

    14th International society for microbial ecology  2012.8 

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  • 亜硝酸酸化細菌Nitrospiraの新規分離培養手法とその実証

    藤谷拓嗣, 牛木章友, 常田 聡, 青井議輝

    第27回日本微生物生態学会  2011.10 

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  • Selective and highly enrichment of nitrite-oxidizing bacteria Nitrospira International conference

    Hirotsugu Fujitani, Satoshi Tsuneda, Yoshiteru Aoi

    2nd International conference on nitrification  2011.7 

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  • Selective and highly enrichment of nitrite-oxidizing bacteria Nitrospira Invited International conference

    Hirotsugu Fujitani, Satoshi Tsuneda, Yoshiteru Aoi

    Early career scientist workshop ICoN2  2011.7 

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  • Growth Characteristics of Uncultured Nitrite-Oxidizing Nitrospira

    2010.11 

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  • Selective and highly efficient enrichment of uncultured Nitrospira International conference

    Hirotsugu Fujitani, Yoshiteru Aoi, Satoshi Tsuneda

    13th International society for microbial ecology  2010.8 

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  • 難培養性亜硝酸酸化細菌Nitrospiraの分離培養

    藤谷拓嗣, 青井議輝, 常田 聡

    第25回日本微生物生態学会  2009.11 

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  • Cultivation of uncultured nitrite-oxidizing bacteria International conference

    Hirotsugu Fujitani, Yoshiteru Aoi, Satoshi Tsuneda

    1st International Conference on Nitrification  2009.7 

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  • 難培養性亜硝酸酸化細菌Nitrospiraの新規分離培養法

    藤谷拓嗣, 青井議輝, 常田 聡

    第43回日本水環境学会年会  2009.3 

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  • Cultivation of uncultured nitrite oxidizing bacteria International conference

    Hirotsugu Fujitani, Yoshiteru Aoi, Satoshi Tsuneda

    12th International society for microbial ecology  2008.8 

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  • 多摩川河口干潟の窒素循環に寄与する微生物群の解析

    藤谷拓嗣, 石川大祐, 田嶋良行, 小関祥子, 陶 景倫, 小島茂明, 浦川秀敏, 常田 聡

    第41回日本水環境学会年会  2007.3 

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Awards

  • 2020年度環境バイオテクノロジー学会奨励賞

    2020.6   Japan Society for Environmental Biotechnology  

  • Best Poster Award

    2019.10   6th International Conference on Nitrification   A flexible survival strategy of ammonia-oxidizing bacteria by growth heterogeneity at a single cell level

    Rino Isshiki

  • Poster Award (Second prize award)

    2019.10   6th International Conference on Nitrification   Enrichment of comammox and canonical Nitrospira from acidic soil: Insights into niche differentia-tion and adaptation to low pH

    Yu Takahashi

  • 優秀発表賞(ポスター)

    2019.9   第33回日本微生物生態学会   細胞ごとに増殖速度のばらつきが大きいアンモニア酸化細菌のしなやかな生存戦略

    一色理乃

  • 優秀発表賞(ポスター)

    2017.8   環境微生物系学会合同大会2017   ゲノムから推定する遅増殖性Nitrotogaの活性促進因子

    石井拳人

  • Best Poster Award

    2015.7   4th International Conference on Nitrification (ICoN4)   Isolation and successful subculture of Nitrosomonas mobilis lineage: Recovery of nature's lost treasure since 1970's

    FUJITANI, Hirotsugu

  • 博士研究奨励賞(オルガノ賞)

    2013.11   第16回日本水環境学会シンポジウム   難培養性亜硝酸酸化細菌Nitrospiraを標的とした革新的分離培養戦略

    藤谷 拓嗣

  • Best Poster Award

    2013.7   5th Quadrennial International Water Association Specialty Conference on Microbial Ecology and Water Engineering   An innovative cultivation and isolation strategy for uncultured nitrite-oxidizing bacteria of the genus Nitrospira

    FUJITANI, Hirotsugu

  • Poster Award

    2012.9   Isolation of previously uncultured Nitrospira unveils the physiology of nitrite-oxidizing bacteria in wastewater treatment process

    FUJITANI, Hirotsugu

  • 最優秀発表賞(ポスター)

    2009.11   第25回日本微生物生態学会   難培養性亜硝酸酸化細菌Nitrospiraの分離培養

    藤谷 拓嗣

  • 優秀発表賞(クリタ賞)

    2009.3   第43回日本水環境学会   難培養性亜硝酸酸化細菌Nitrospiraの新規分離培養法

    藤谷 拓嗣

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Research Projects

  • 陸域窒素循環のミッシングリンクを解く

    2021.4 - 2023.3

    日本学術振興会  科学研究費助成事業  令和元年度基盤研究(A) 

    多胡香奈子

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    Grant type:Competitive

    Grant amount: \44980000 ( Direct Cost: \34600000 、 Indirect Cost: \10380000 )

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  • 培養技術とゲノム解析で紐解く硝化界のダークマター「完全アンモニア酸化細菌」

    2019.4 - 2022.3

    日本学術振興会科学研究費助成事業  令和元年度若手研究 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \4160000 ( Direct Cost: \3200000 、 Indirect Cost: \960000 )

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  • 新学術領域研究令和2年度「先進ゲノム支援」

    2020.4 - 2021.3

    日本学術振興会  科学研究費助成事業 

    Hirotsugu Fujitani

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    Grant type:Competitive

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  • 2020年度国際学術誌投稿助成

    2021.2    

    中央大学 

    藤谷拓嗣

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    Grant type:Competitive

    Grant amount: \200000

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  • 選択培地と集積培養に依存しない硝化菌の純粋分離

    2018.6 - 2020.3

    日本学術振興会科学研究費助成事業  平成30年度挑戦的研究(萌芽) 

    諏訪 裕一

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    Grant type:Competitive

    Grant amount: \6370000 ( Direct Cost: \4900000 、 Indirect Cost: \1470000 )

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  • 革新的分離培養技術で解明する硝化細菌の生理生態と多様性

    2016.4 - 2019.3

    日本学術振興会科学研究費助成事業  平成28年度若手研究B 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \4030000 ( Direct Cost: \3100000 、 Indirect Cost: \930000 )

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  • メタゲノム解析から発見された新規なComammox細菌の分離培養と性状解析

    2017.12 - 2018.11

    公益財団法人発酵研究所  平成29年度(2017年度)研究者招聘・留学助成 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \3000000

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  • 部分硝化の制御・安定化を志向した多様な硝化細菌の生理学的解析

    2017.10 - 2018.9

    公益財団法人クリタ水・環境科学振興財団  2017年度国内研究助成 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \1000000

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  • 新学術領域研究平成29年度「先進ゲノム支援」

    2017.9 - 2018.3

    日本学術振興会  科学研究費助成事業 

    藤谷 拓嗣

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  • 平成29年度「先進ゲノム支援」

    2017.9 - 2018.3

    日本学術振興会科学研究費助成事業  新学術領域研究 

    藤谷 拓嗣

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  • 窒素循環の制御とN2O生成の抑制を志向した硝化・脱窒菌の網羅的獲得

    2017.1 - 2017.12

    公益財団法人前川報恩会  平成28年度学術研究助成 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \1555500

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  • 部分硝化の制御・安定化を志向した多様な硝化細菌の生理学的解析

    2016.10 - 2017.9

    公益財団法人クリタ水・環境科学振興財団  2016年度国内研究助成 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \930000

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  • 新学術領域研究平成28年度「先進ゲノム支援」

    2016.9 - 2017.3

    日本学術振興会  科学研究費助成事業 

    藤谷 拓嗣

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    Grant type:Competitive

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  • 平成28年度「先進ゲノム支援」

    2016.9 - 2017.3

    日本学術振興会科学研究費助成事業  新学術領域研究 

    藤谷 拓嗣

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  • N2O生成に関与する硝化・脱窒細菌の網羅的遺伝子解析と分離培養

    2016.2 - 2016.12

    一般財団法人前川報恩会  平成27年度学術研究助成 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \1500000

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  • 環境中に潜在する硝化細菌の網羅的・高速ハンティング

    2015.7 - 2016.3

    早稲田大学  2015年度特定課題研究助成費(特定課題B) 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \360000

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  • 硝化細菌のマイクロコロニー形成機構の解明

    2015.4 - 2016.3

    早稲田大学  2015年度特定課題研究助成費(基礎助成) 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \240000

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  • 窒素循環に寄与する新規なアンモニア酸化細菌・古細菌の分離培養と機能解明

    2014.7 - 2015.3

    早稲田大学  2014年度特定課題研究助成費(特定課題B) 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \384000

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  • nxrB遺伝子に基づく新規な亜硝酸酸化細菌の探索

    2014.4 - 2015.3

    早稲田大学  2014年度特定課題研究助成費(基礎助成) 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \270000

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  • NxrB遺伝子に基づく新規な亜硝酸酸化細菌の探索・分離培養

    2014.4 - 2015.2

    公益財団法人日本科学協会  平成26年度笹川科学研究助成(学術研究部門,生物系) 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \700000

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  • 未培養性アンモニア酸化細菌・古細菌を標的としたハイスループット新規分離培養手法の開発

    2013.6 - 2014.3

    早稲田大学  2013年度特定課題研究助成費(新任の教員等) 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \180000

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  • 窒素循環に寄与する難培養性亜硝酸酸化細菌Nitrospiraの分離培養

    2011.4 - 2012.2

    公益財団法人日本科学協会  平成23年度笹川科学研究助成(学術研究部門,生物系) 

    藤谷 拓嗣

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \490000

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Other

  • 中学校・高等学校教諭専修免許状(理科)

    2009.3    

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  • 中学校・高等学校教諭1種免許状(理科)

    2007.3    

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Teaching Experience

  • 理工学基礎実験IB

  • 理工学基礎実験IA

  • 生命医科学実験I

  • 環境生物学実験

  • 環境応用微生物学

Social Activities

  • 私たちの生活に身近な微生物の世界

    Role(s): Lecturer

    公益財団法人文京アカデミー  文京アカデミア講座  2022.6 - 2022.7

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    Audience: General

    Type:Seminar, workshop

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  • Local organizing committee members

    Role(s): Organizing member

    国際水協会(IWA)  Microbial Ecology and Water Engineering 2019  2019.11    

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    Type:Seminar, workshop

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  • かくれているものは,なぁに?

    Role(s): Organizing member, Demonstrator

    国立研究開発法人産業技術総合研究所  2019年産総研一般公開  2019.7    

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    Type:University open house

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  • 身のまわりの光を見てみよう!

    Role(s): Lecturer, Demonstrator

    国立研究開発法人科学技術振興機構(JST)  新宿SPP(Science Partnership Project)新宿区立西早稲田中学校2年生対象  2017.11    

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    Type:Visiting lecture

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  • Local organizing committee members

    Role(s): Organizing member

    公益社団法人日本生物工学会  第69回日本生物工学会大会  2017.9    

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    Type:Seminar, workshop

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  • 光る生物の解明をしよう!

    Role(s): Lecturer, Demonstrator

    国立研究開発法人科学技術振興機構(JST)  新宿SPP(Science Partnership Project)新宿区立牛込第一中学校2年生対象  2014.11    

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    Type:Visiting lecture

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  • Local organizing committee members

    Role(s): Organizing member

    公益社団法人日本水環境学会  Water and Environment Technology Conference 2014  2014.6    

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    Type:Seminar, workshop

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  • 夢を叶えるために,今必要なこと

    Role(s): Lecturer

    東京都青梅市立第二中学校  平成25年度第3学年進路講演会  2014.3    

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    Type:Lecture

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  • Local organizing committee members

    Role(s): Organizing member

    Nitrification Network  3rd International conference on nitrification  2013.9    

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    Type:Seminar, workshop

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  • 目には見えないミクロの世界をのぞいてみよう!―未知なる世界への挑戦―

    Role(s): Presenter, Lecturer, Organizing member, Demonstrator

    早稲田大学理工学術院  第23回ユニラブ(小中学生のための科学実験教室)  2010.8    

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    Type:Visiting lecture

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