Updated on 2024/02/15

写真a

 
SUZUKI Hiroaki
 
Organization
Faculty of Science and Engineering Professor
Other responsible organization
Precision Engineering Course of Graduate School of Science and Engineering, Master's Program
Precision Engineering Course of Graduate School of Science and Engineering, Doctoral Program
Contact information
The inquiry by e-mail is 《here
External link

Degree

  • 博士(工学) ( 東京大学 )

  • 修士(工学) ( 東京大学 )

Education

  • 2002.9
     

    The University of Tokyo   Graduate School, Division of Engineering   doctor course   finished without a degree after completion of required course credits

  • 1999.3
     

    The University of Tokyo   Graduate School, Division of Engineering   master course   completed

  • 1997.3
     

    The University of Tokyo   Faculty of Engineering   graduated

  • 1992.3
     

    愛知県立旭丘高等学校   graduated

Research History

  • 2016.4 -  

    中央大学理工学部 教授

  • 2013.4 - 2016.3

    中央大学理工学部 准教授

  • 2009.10 - 2015.3

    日本科学技術振興機構 戦略的創造研究推進事業(ERATO四方プロジェクト) グループリーダー

  • 2007.8 - 2013.3

    大阪大学大学院情報科学研究科 准教授

  • 2003.11 - 2007.7

    東京大学生産技術研究所 助教

  • 2003.5 - 2003.10

    東京大学生産技術研究所 学術研究支援員

  • 2003.4    

    東京大学生産技術研究所 博士研究員

  • 2002.9 - 2003.3

    東京大学大学院工学系研究科 学術研究支援員

  • 2000.4 - 2002.3

    日本学術振興会 特別研究員DC2 (東京大学大学院工学系研究科)

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Professional Memberships

  • 細胞を創る研究会

  • 化学とマイクロ・ナノシステム学会

  • American Chemical Society

  • 日本生物物理学会

  • 日本機械学会

  • 電気学会

  • 日本膜学会

  • 精密工学会

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Research Interests

  • Micro and Nano Devices

  • Biophysics

  • Micro and Nano Bioscience

Research Areas

  • Nanotechnology/Materials / Nano/micro-systems  / ナノマイクロシステム

  • Life Science / Biophysics  / 生物物理学

Papers

  • Gut-liver interaction study on an all-polydimethylsiloxane microfluidic device integrating intestinal paracellular permeability assay Reviewed

    Ryuya Kida, Alan Rajendran, Mamiko Tsugane, Jean-Charles Duclos-Vallée, Maxime M Mahe, Sakina Bensalem, Hiroaki Suzuki, Bruno Le Pioufle

    Talanta Open   9   100289 - 100289   2024.8

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.talo.2024.100289

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  • Horizontal and vertical microchamber platforms for evaluation of the paracellular permeability of an epithelial cell monolayer Reviewed

    Ryuya Kida, Mamiko Tsugane, Hiroaki Suzuki

    Lab on a Chip   24 ( 3 )   572 - 583   2024

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Royal Society of Chemistry (RSC)  

    Microchamber devices that enable evaluation of paracellular permeability while observing high resolution epithelial cell morphology are developed.

    DOI: 10.1039/d3lc00855j

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  • Identifying Conditions for Protein Synthesis Inside Giant Vesicles Using Microfluidics toward Standardized Artificial Cell Production Reviewed

    Ryota Ushiyama, Satoshi Nanjo, Mamiko Tsugane, Reiko Sato, Tomoaki Matsuura, Hiroaki Suzuki

    ACS Synthetic Biology   13 ( 1 )   68 - 76   2023.11

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Chemical Society (ACS)  

    DOI: 10.1021/acssynbio.3c00629

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  • Single-cell trapping and retrieval in open microfluidics Reviewed

    Tomoki Murakami, Hiroto Teratani, Dai’ichiro Aoki, Masao Noguchi, Mamiko Tsugane, Hiroaki Suzuki

    iScience   26 ( 11 )   108323 - 108323   2023.11

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.isci.2023.108323

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  • In-Plane Orientational Control of Electronic Components Using Pattern Complementarity in a Self-Assembling System Reviewed

    Kaito Nakayama, Tatsuya Hikida, Hiroaki Suzuki

    Journal of Microelectromechanical Systems   32 ( 5 )   454 - 460   2023.10

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Institute of Electrical and Electronics Engineers (IEEE)  

    DOI: 10.1109/jmems.2023.3296381

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  • Investigation of the vibration-induced local flow around a micro-pillar under various vibration conditions Reviewed

    K. Kaneko, Z. Huang, T. Sato, N. Ujikawa, T. Hayakawa, Y. Hasegawa, H. SUzuki

    Mechanical Engineering Journal   2022.12

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Japan Society of Mechanical Engineers  

    DOI: 10.1299/mej.22-00223

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  • Endocytosis of Coacervates into Liposomes Reviewed

    Tiemei Lu, Susanne Liese, Ludo Schoenmakers, Christoph A. Weber, Hiroaki Suzuki, Wilhelm T. S. Huck, Evan Spruijt

    J. Am. Chem. Soc.   2022.7

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    DOI: 10.1021/jacs.2c04096

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  • Plug-and-Play Microfluidic Production of Monodisperse Giant Unilamellar Vesicles Using Droplet Transfer across Water-Oil Interface Reviewed

    R. Ushiyama, K. Koiwai, H. Suzuki

    Sensors and Actuators B: Chemical   355 ( 15 )   131281 - 131281   2021.12

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    DOI: 10.1016/j.snb.2021.131281

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  • Applying deterministic lateral displacement cell separation on immune cells of Marine shrimp Reviewed

    Tomoki Murakami, Keiichiro Koiwai, Hiroaki Suzuki

    Sensors and Actuators B: Chemical   347   130587   2021.11

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    DOI: 10.1016/j.snb.2021.130587

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  • Sizing of Giant Unilamellar Vesicles Using a Metal Mesh with a High Opening Ratio Reviewed

    K. Shinohara, T. Okita, M. Tsugane, T. Kondo, H. Suzuki

    Chemistry and Physics of Lipids   241   105148   2021.11

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    DOI: 10.1016/j.chemphyslip.2021.105148

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  • Single-cell RNA-seq analysis reveals penaeid shrimp hemocyte subpopulations and cell differentiation process International journal

    K. Koiwai, T. Koyama, S. Tsuda, A. Toyoda, K. Kikuchi, H. Suzuki, R. Kawano

    eLife   10   e66954   2021.6

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:eLife Sciences Publications Ltd.  

    Crustacean aquaculture is expected to be a major source of fishery commodities in the near future. Hemocytes are key players of the immune system in shrimps; however, their classification, maturation, and differentiation are still under debate. To date, only discrete and inconsistent information on the classification of shrimp hemocytes has been reported, showing that the morphological characteristics are not sufficient to resolve their actual roles. Our present study using single-cell RNA sequencing revealed six types of hemocytes of Marsupenaeus japonicus based on their transcriptional profiles. We identified markers of each subpopulation and predicted the differentiation pathways involved in their maturation. We also predicted cell growth factors that might play crucial roles in hemocyte differentiation. Different immune roles among these subpopulations were suggested from the analysis of differentially expressed immune-related genes. These results provide a unified classification of shrimp hemocytes, which improves the understanding of its immune system.

    DOI: 10.7554/eLife.66954

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  • Elucidating the Membrane Dynamics and Encapsulation Mechanism of Large DNA Molecules Under Molecular Crowding Conditions Using Giant Unilamellar Vesicles Invited Reviewed

    Mamiko Tsugane, Hiroaki Suzuki

    ACS Synthetic Biology   9 ( 10 )   2819 - 2827   2020.9

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    The conservation throughout evolution of membrane-bound structures that encapsulate genomic material indicates the existence of a simple, physical mechanism that facilitates the enclosing of long-stranded DNA by lipid bilayers. This study aimed to elucidate such a mechanism by investigating how molecular crowding promotes the spontaneous enveloping of model DNA into lipid bilayer membranes. Using fluorescence microscopy and giant unilamellar vesicles (GUVs) we showed that a 166 kb DNA molecule coencapsulated with a model crowder attaches to the inner membrane of the GUVs as they osmotically deflate and after the DNA–membrane complex buds out. The set of results is consistent with the hypothesis that the depletion volume effect is responsible for the spontaneous encapsulation of DNA in the GUVs. This phenomenon may offer novel insights into the basic mechanisms governing membrane encapsulation of long-stranded nucleic acids found in celluar sytems that are independent of genetic control.

    DOI: 10.1021/acssynbio.0c00360

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  • Selective self-assembly of three-component system based on hydrophilic/hydrophobic patterning Reviewed

    Taiki Okuyama, Tatsuya Hikida, Taiji Okano, Hiroaki Suzuki

    Sensors and Actuators A: Physical   312   112143   2020.9

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    This paper presents a demonstration of the selective bonding of three different millimeter-scale components by using complementary patterning of hydrophilic and hydrophobic surfaces. The hydrophobic surface of millimeter-scale components fabricated from hydrophobic polydimethylsiloxane (PDMS) was made partially hydrophilic with a designed pattern by exposure to an excimer light through a stencil mask. The oil, as an adhesive, was only deposited on the hydrophobic surface. We prepared 30 components with 3 patterns, which were agitated in water by using a computer-controlled propeller stirrer. As a result, we obtained a significantly higher yield of correct bonds compared to erroneous bonds under an appropriate stirring condition. The result can be explained through the contrast in bonding strength between the correct and erroneous bonds. The design principle of using concentric patterns serves as a basic strategy for realizing programmed self-assembly with selective bonding patterns.

    DOI: 10.1016/j.sna.2020.112143

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  • Deformation Dynamics of Giant Unilamellar Vesicles in the Large Surface-to-Volume Ratio Regime: The Emergence of Neuron-like Morphology Reviewed

    Kaoru Koseki, Hiroaki Suzuki

    Langmuir   36 ( 22 )   6238 - 6244   2020.5

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    Deformation of liposomes, or lipid vesicles, has been investigated extensively in terms of the thermodynamic equilibrium of the bending energy of the lipid bilayer membrane. However, the range of such deformation in previous literature has been limited within the moderate surface-to-volume ratio of the vesicles, in which axisymmetric shapes are dominant. Here, we show that neuron-like morphology, in which many lipid tubes extend radially from the mother vesicle, becomes dominant upon the slow osmotic shrinkage of giant unilamellar vesicles (GUVs) initially larger than several tens of micrometers. We show that, in the time-lapse confocal imaging, the emergence of lipid tubes is initiated from the instability that appeared along the annular rim of the flat stomatocyte shape. Since these deformation dynamics into the neuron-like morphology resemble that of the milk-crown formation in liquid splashing, we discuss that the Rayleigh–Plateau capillary instability drives this transformation into a nonaxisymmetric shape.

    DOI: 10.1021/acs.langmuir.0c00872

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  • A simple microfluidic device for live-imaging of the vertical section of epithelial cells Reviewed

    Seigo Araki, Masayoshi Nakano, Mamiko Tsugane, Fumiko Sunaga, Mitsuru Hattori, Masahiro Nakano, Takeharu Nagai, Hiroaki Suzuki

    Analyst   145 ( 2 )   667 - 674   2019.11

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    We investigated the capability of simple microfluidic devices with trenches having vertical sidewalls for live-cell fluorescence imaging of adherent cells. An epithelial cell line that forms a two-dimensional (2D) sheet was cultured to adhere to the vertical sidewall so that its vertical section can be imaged directly using ordinal inverted-type laser-scanning microscopy. The material and the structure of the device were characterized. We show that the detailed distribution of intracellular organelles, such as microtubules and mitochondria, and of intercellular apparatus, such as claudin and zonula occludens, can be imaged with high spatio-temporal resolution with a single scan.

    DOI: 10.1039/C9AN02165E

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  • Ejection of Large Particulate Materials from Giant Unilamellar Vesicles Induced by Electropulsation Reviewed

    Shota Katsuta, Taiji Okano, Kiiichiro Koiwai, Hiroaki Suzuki

    Langmuir   35 ( 40 )   13196 - 13204   2019.9

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    Electroporation or electropermealization is a technique to open pores in the lipid bilayer membrane of cells and vesicles transiently to increase its permeability to otherwise impermeable molecules. However, the upper size limit of the materials permeable through this operation has not been studied in the past. Here, we investigate the size of the material that can be released (ejected) from giant unilamellar vesicles (GUVs) upon electrical pulsation. We confirm that the volume of GUV shrinks in a stepwise manner upon periodical pulsation, in accordance with previous studies. When the same operation is applied to GUVs that encapsulate microbeads, we find that beads as large as 20 μm can be ejected across the membrane without rupturing the whole GUV structure. We also demonstrate that functional bioactive particulate materials, such as gel balls, vesicles, and cells can be encapsulated in and ejected from GUVs. We foresee that this phenomenon can be applied to precisely regulate the time and location of release of these particulate materials in the microenvironment.

    DOI: 10.1021/acs.langmuir.9b01617

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  • Polymer-Induced Self-Assembly of a Three-Dimensional Mesoscale Structure Reviewed

    Ryota Kawai, Yaoki Mori, Hiroaki Suzuki

    J. Microelectromechanical Systems   28 ( 4 )   678 - 684   2019.8

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    Three-dimensional (3D) self-assembly of mesoscale parts with tens to hundreds of micrometer scales, independent of the material properties, is demonstrated by simply adding an inert polymer, i.e., polyethylene glycol, with large molecular weight (4MDa) at a concentration of micrograms per milliliters. It is most likely that the depletion volume effect, a thermodynamical effect to maximize the entropy of the system, induces face-to-face bonds with microfabricated parts having flat faces. The optimum polymer concentration range yielding high assembling efficiency is found, where the bonding energy is large enough to stabilize the bonding and viscosity is small enough to allow frequent collisions. Using this strategy, a site-specific assembly of 60 μm cubic parts in cavities engraved on large receptor parts, distributed on either 2D or 3D faces, is successfully demonstrated.

    DOI: 10.1109/JMEMS.2019.2919230

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  • Assembly of Microparticles to Patterned Trenches Using the Depletion Volume Effect Reviewed

    Yaoki Mori, Ryota Kawai, Hiroaki Suzuki

    Micromachines   10 ( 7 )   428   2019.6

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI  

    In this paper, we demonstrate that 20 μm microbeads can be preferentially assembled into substrate trenches of similar width by employing a polymer (depletant) that induces the depletion volume effect (depletion attraction). In previous works, we proved that this strategy is useful to assemble mesoscale parts in a site-specific manner. Here, we show that it is also applicable to assemble functional parts, such as fluorescent particles, into trenches engraved on the surface of two- and three-dimensional template components. A convenient advantage of this strategy is that it is independent of material properties for assembling mesoscale functional components into desired patterns.

    DOI: 10.3390/mi10070428

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  • Numerical and Experimental Analyses of Three- Dimensional Unsteady Flow around a Micro-Pillar Subjected to Rotational Vibration Reviewed

    K. Kaneko, T. Osawa, Y. Kametani, T. Hayakawa, Y. Hasegawa, H. Suzuki

    Micromachines   9 ( 12 )   668   2018.12

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    The steady streaming (SS) phenomenon is gaining increased attention in the microfluidics community, because it can generate net mass flow from zero-mean vibration. We developed numerical simulation and experimental measurement tools to analyze this vibration-induced flow, which has been challenging due to its unsteady nature. The validity of these analysis methods is confirmed by comparing the three-dimensional (3D) flow field and the resulting particle trajectories induced around a cylindrical micro-pillar under circular vibration. In the numerical modeling, we directly solved the flow in the Lagrangian frame so that the substrate with a micro-pillar becomes stationary, and the results were converted to a stationary Eulerian frame to compare with the experimental results. The present approach enables us to avoid the introduction of a moving boundary or infinitesimal perturbation approximation. The flow field obtained by the micron-resolution particle image velocimetry (micro-PIV) measurement supported the three-dimensionality observed in the numerical results, which could be important for controlling the mass transport and manipulating particulate objects in microfluidic systems.

    DOI: 10.3390/mi9120668

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  • Selective Bonding Method for Self-Assembly of Heterogeneous Components Using Patterned Surfaces Reviewed

    K. Kimura, T. Okuyama, T. Okano, H. Suzuki

    Sensors Actuators A: Physical   279 ( 15 )   306 - 312   2018.8

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  • Reverse Transcription Polymerase Chain Reaction in Giant Unilamellar Vesicles Reviewed

    M. Tsugane, H. Suzuki

    Scientific Reports   8   9214   2018.6

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  • A fluidics-based impact sensor Reviewed

    Daigo Takahashi, Keisuke Hara, Taiji Okano, Hiroaki Suzuki

    PLoS ONE   13 ( 4 )   2018.4

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    Microelectromechanical systems (MEMS)-based high-performance accelerometers are ubiquitously used in various electronic devices. However, there is an existing need to detect physical impacts using low-cost devices with no electronic circuits or a battery. We designed and fabricated an impact sensor prototype using a commercial stereolithography apparatus that only consists of a plastic housing and working fluids. The sensor device responds to the instantaneous acceleration (impact) by deformation and pinch off of a water droplet that is suspended in oil in a sensor cavity. We tested the various geometrical and physical parameters of the impact sensor to identify their relations to threshold acceleration values. We show that the state diagram that is plotted against the dimensionless Archimedes and Bond numbers adequately describes the response of the proposed sensor.

    DOI: 10.1371/journal.pone.0195741

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  • Deformation Modes of Giant Unilamellar Vesicles Encapsulating Biopolymers Reviewed

    Taiji Okano, Koya Inoue, Kaoru Koseki, Hiroaki Suzuki

    ACS Synthetic Biology   7 ( 2 )   739 - 747   2018.1

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  • Self-assembly of artificially manufactured microcomponents using the entropic effect Reviewed

    U. Okabe, T. Okano, H. Suzuki

    SENSORS AND ACTUATORS A-PHYSICAL   254   43 - 53   2017.2

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE SA  

    The excluded volume effect, or depletion attraction, is the phenomenon describing induced aggregation of colloidal particles suspended in a densely crowded macromolecule solution. In this work, we attempted to utilize this effect for the self-assembly of artificially manufactured microcomponents on a 10-100 mu m scale. The bonding energy does not originate from the nature of the surfaces, but is generated by an increase of the translational entropy of macromolecules in solution. Thus, simple immersion of the microcomponents in the macromolecule solution allowed us to observe their assembly, based on shape complementarity, to minimize the free energy of the system. However, it became apparent that elaborate design is required for specific bonding between complementary shapes, in addition to merely increasing the contact surface area. (C) 2016 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.sna.2016.11.027

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  • One-step micromolding of complex 3D microchambers for single-cell analysis Reviewed

    Hiroaki Suzuki, Kenta Mitsuno, Katsuyuki Shiroguchi, Mamiko Tsugane, Taiji Okano, Tetsuji Dohi, Tomoaki Tsuji

    Lab on a Chip   17 ( 4 )   647 - 652   2017

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Royal Society of Chemistry  

    Herein we examined the extent of replicability of the PDMS microchamber device transferred from the master mold with complex 3D structures fabricated via micro stereolithography. Due to the elastomeric properties of PDMS, the reversely tapered micromold, with the diameter ratio of ∼5 from the largest to the narrowest part, was precisely transferred without breaking. We obtained the mathematical model to estimate the stress exerted on the mold during the demolding process. Finally, we tested the applicability of this unusual microchamber for single-cell trapping and an enzyme assay.

    DOI: 10.1039/c6lc01313a

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  • Shape Transformations of Lipid Vesicles by Insertion of Bulky-Head Lipids Reviewed

    Soichiro Tsuda, Tatsuya Sakakura, Satoshi Fujii, Hiroaki Suzuki, Tetsuya Yomo

    PLOS ONE   10 ( 7 )   e0132963   2015.7

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    Lipid vesicles, in particular Giant Unilamellar Vesicles (GUVs), have been increasingly important as compartments of artificial cells to reconstruct living cell-like systems in a bottom-up fashion. Here, we report shape transformations of lipid vesicles induced by polyethylene glycol-lipid conjugate (PEG lipids). Statistical analysis of deformed vesicle shapes revealed that shapes vesicles tend to deform into depended on the concentration of the PEG lipids. When compared with theoretically simulated vesicle shapes, those shapes were found to be more energetically favorable, with lower membrane bending energies than other shapes. This result suggests that the vesicle shape transformations can be controlled by externally added membrane molecules, which can serve as a potential method to control the replications of artificial cells.

    DOI: 10.1371/journal.pone.0132963

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  • Microchamber device for detection of transporter activity of adherent cells Reviewed

    Mamiko Tsugane, Etsuko Uejima, Hiroaki Suzuki

    Frontiers in Bioengineering and Biotechnology   3   2015

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Frontiers Media S.A.  

    We present a method to detect the transporter activity of intact adherent cells using a microchamber device. When adherent cells are seeded onto the poly-di-methyl siloxane substrate having microchambers with openings smaller than the size of a cell, the cells form a confluent layer that covers the microchambers, creating minute, confined spaces. As substances exported across the cell membrane accumulate, transporter activity can be detected by observing the fluorescence intensity increase in the microchamber. We tested the microchamber device with HeLa cells over-expressing MDR1, an ATP-binding cassette transporter, and succeeded in detecting the transport of fluorescence-conjugated paclitaxel, the anti-cancer drug, at the single-cell level.

    DOI: 10.3389/fbioe.2015.00032

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  • Stochasticity in Gene Expression in a Cell-Sized Compartment Reviewed

    Kz. Nishimura, T. Mtasuura, T. Sunami, S. Tsuru, H. Suzuki, T. Yomo

    ACS Synth. Biol.   2014.10

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  • Liposome-Based Liquid Handling Platform Featuring Addition, Mixing, and Aliquoting of Femtoliter Volumes Reviewed

    Hideaki Shiomi, Soichiro Tsuda, Hiroaki Suzuki, Tetsuya Yomo

    PLOS ONE   9 ( 7 )   2014.7

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    This paper describes the utilization of giant unilamellar vesicles (GUVs) as a platform for handling chemical and biochemical reagents. GUVs with diameters of 5 to 10 mm and containing chemical/biochemical reagents together with inert polymers were fused with electric pulses (electrofusion). After reagent mixing, the fused GUVs spontaneously deformed to a budding shape, separating the mixed solution into sub-volumes. We utilized a microfluidic channel and optical tweezers to select GUVs of interest, bring them into contact, and fuse them together to mix and aliquot the reaction product. We also show that, by lowering the ambient temperature close to the phase transition temperature T-m of the lipid used, daughter GUVs completely detached (fission). This process performs all the liquid-handing features used in bench-top biochemistry using the GUV, which could be advantageous for the membrane-related biochemical assays.

    DOI: 10.1371/journal.pone.0101820

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  • Statistical analysis of vesicle morphology dynamics based on a free energy landscape Reviewed

    Soichiro Tsuda, Hiroaki Suzuki, Tetsuya Yomo

    SOFT MATTER   10 ( 32 )   6038 - 6046   2014

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ROYAL SOC CHEMISTRY  

    We here present a method to reconstruct effective free energy landscapes (FELs) of lipid vesicles from the statistical analysis of a large number of microscope images. This method, not only allows us to define possible energy landscapes, but also highlights minority vesicle shapes that were otherwise hidden in the majority. When compared with temporal evolution of deforming lipid vesicles, it was found that the trajectory of deforming vesicles was in accordance with the reconstructed landscape, in which the minority shapes play a key role. When compared with theoretical models, it revealed that the vesicle shapes characterised in the reconstructed FELs were consistent with the theoretically predicted shapes. These results suggest that the FEL analysis can be a useful tool to investigate the morphological dynamics of lipid vesicles, in conjunction with other analytical methods.

    DOI: 10.1039/c4sm00992d

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  • Identification of giant unilamellar vesicles with permeability to small charged molecules Reviewed

    Koji Nishimura, Tomoaki Matsuura, Takeshi Sunami, Satoshi Fujii, Kazuya Nishimura, Hiroaki Suzuki, Tetsuya Yomo

    RSC ADVANCES   4 ( 66 )   35224 - 35232   2014

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ROYAL SOC CHEMISTRY  

    The permeability of giant unilamellar vesicle (GUV) membranes to various solutes was investigated at the single-vesicle level. Membrane permeability has primarily been studied by obtaining the average of vesicle ensembles using small or large unilamellar vesicles (SUVs and LUVs, respectively) <1 mu m in diameter. The average properties observed for biological molecules or systems may not necessarily represent those of individual vesicles. In addition, although the GUV (>1 mu m) is considered to be a primitive cell model, its membrane permeability has rarely been investigated. We investigated the permeation of various molecules, including amino acids and mononucleotides, through more than 20 000 GUV membranes composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) using flow cytometry. We observed a general trend of lower membrane permeability for polar or charged molecules than for nonpolar molecules, which is consistent with previous studies. However, we found that the lower permeation of charged molecules resulted from the presence of at least two distinct GUV populations; the larger population consisted of impermeable GUVs, and the smaller population consisted of those with a high permeability. The presence of phospholipid vesicles highly permeable to charged and small molecules (<3 nm) was found through the single vesicular measurement. The observed permeability of the GUVs may have played an essential role in the self-reproduction and evolution of primitive cells.

    DOI: 10.1039/c4ra05332j

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  • Modification of an Amplification Reaction in Recursively Dynamic Compartments Driven by Stirring Reviewed

    T. Ichii, G. Tanahashi, H. Suzuki, T. Yomo

    Anal. Chem.   85 ( 24 )   12002 - 12010   2013.11

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  • Modification of an Amplification Reaction in Recursively Dynamic Compartments Driven by Stirring Reviewed

    T. Ichii, G. Tanahashi, H. Suzuki, T. Yomo

    Anal. Chem.   85 ( 24 )   12002 - 12010   2013.11

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  • Cell-free protein synthesis in a microchamber revealed the presence of an optimum compartment volume for high-order reactions Reviewed

    T. Okano, T. Matsuura, H. Suzuki, T. Yomo

    ACS Synth. Biol.   3 ( 6 )   347 - 352   2013.8

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  • Cell-free protein synthesis in a microchamber revealed the presence of an optimum compartment volume for high-order reactions Reviewed

    T. Okano, T. Matsuura, H. Suzuki, T. Yomo

    ACS Synth. Biol.   online   2013.8

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  • 脂質膜ダイナミクスからみる原始細胞の起源 Reviewed

    鈴木宏明

    生物物理   53 ( 3 )   134 - 139   2013.6

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  • Bio-inspired three-dimensional self-patterning of functional coatings for PDMS microfluidics Reviewed

    Tianzhun Wu, Hiroaki Suzuki, Yuquan Su, Zikang Tang, Liang Zhang, Tetsuya Yomo

    Soft Matter   9 ( 13 )   3473 - 3477   2013.4

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    We report a facile, versatile and highly efficient self-patterning method for depositing functional coatings for poly(dimethylsiloxane) (PDMS) microfluidics, inspired by the wetting phenomena of the desert beetle and plant leaves. Driven by the solvent evaporation, fluoropolymer coatings have been demonstrated to deposit only on the roughened PDMS surfaces with exact coverage, conformal thickness, no clogging and good bonding strength, which significantly suppressed the swelling of PDMS by the organic solvent and the nonspecific adsorption of the fluorescent dye. © 2013 The Royal Society of Chemistry.

    DOI: 10.1039/c3sm27625b

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  • Effects of compartment size on the kinetics of intracompartmental multimeric protein synthesis Reviewed

    T. Matsuura

    ACS Synthetic Biology   1 ( 9 )   431 - 437   2012.7

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  • Cell-free protein synthesis inside giant unilamellar vesicles analyzed by flow cytometry Reviewed

    K. Nishimura

    Langmuir   28   8426 - 8432   2012.5

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  • Coupling of the fusion and budding of giant phospholipid vesicles containing macromolecules Reviewed

    Hidetoshi Terasawa, Kazuya Nishimura, Hiroaki Suzuki, Tomoaki Matsuura, Tetsuya Yomo

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   109 ( 16 )   5942 - 5947   2012.4

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    Mechanisms that enabled primitive cell membranes to self-reproduce have been discussed based on the physicochemical properties of fatty acids; however, there must be a transition to modern cell membranes composed of phospholipids [Budin I, Szostak JW (2011) Proc Natl Acad Sci USA 108: 5249-5254]. Thus, a growth-division mechanism of membranes that does not depend on the chemical nature of amphiphilic molecules must have existed. Here, we show that giant unilamellar vesicles composed of phospholipids can undergo the coupled process of fusion and budding transformation, which mimics cell growth and division. After gaining excess membrane by electrofusion, giant vesicles spontaneously transform into the budded shape only when they contain macromolecules (polymers) inside their aqueous core. This process is a result of the vesicle maximizing the translational entropy of the encapsulated polymers (depletion volume effect). Because the cell is a lipid membrane bag containing highly concentrated biopolymers, this coupling process that is induced by physical and nonspecific interactions may have a general importance in the self-reproduction of the early cellular compartments.

    DOI: 10.1073/pnas.1120327109

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  • Statistical analysis of the discrete encapsulation of nanomaterials in colloidal capsules Reviewed

    T. Sakakura, K. Nishimura, H. Suzuki, T. Yomo

    Analytical Methods   4   1648 - 1655   2012.4

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  • Size control of giant unilamellar vesicles prepared from inverted emulsion droplets Reviewed

    K. Nishimura, H. Suzuki, T. Toyota, T. Yomo

    Journal of Colloid and Interface Science   376 ( 1 )   119 - 125   2012.3

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  • Fractal-shaped microchannel design for a kinetic analysis of biochemical reaction in a delay line Reviewed

    K. Hirata

    Microfluidics and Nanofluidics   2012.3

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  • Cell-free protein synthesis from a single copy of DNA in a glass microchamber Reviewed

    Taiji Okano, Tomoaki Matsuura, Yasuaki Kazuta, Hiroaki Suzuki, Tetsuya Yomo

    LAB ON A CHIP   12 ( 15 )   2704 - 2711   2012

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    To achieve a cell-mimetic reaction environment, we fabricated and tested quartz microchambers for conducting protein synthesis using an in vitro transcription and translation system, the PURE system. By introducing a glass microchamber and blocking the surface of the chamber with amino acids, the concentration of the synthesized marker protein (green fluorescent protein, GFP) was significantly improved compared to that in the poly(dimethylsiloxane) (PDMS) microchamber. The concentration was below the detection limit in the PDMS microchambers, whereas the glass microchambers yielded 700 nM GFP, representing 41% of the bulk reaction. There was no detectable difference when the GFP synthesis was performed in microchambers with sizes ranging from 40 fL to 7 pL, indicating that the present microchamber system can serve as a cell-sized test tube with a variable reaction volume. Finally, we demonstrated that two different proteins, GFP and beta-galactosidase, can be expressed from single genes in our experimental setup. Quantized and distinctive signals from proteins synthesized from 0, 1, or 2 copies of genes were obtained. The microchamber presented here can be utilized not only to study the effects of compartment volume on protein synthesis but also for the comprehensive analysis of complex biochemical reactions in cell-mimetic environments.

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  • Hydrodynamic trapping of Tetrahymena thermophila for the long-term monitoring of cell behaviors Reviewed

    Itsuka Kumano, Kazufumi Hosoda, Hiroaki Suzuki, Katsuki Hirata, Tetsuya Yomo

    LAB ON A CHIP   12 ( 18 )   3451 - 3457   2012

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    Microfluidic trapping technology has been widely applied for single-cell observation in order to reveal characteristic cell behaviors. However, this strategy has yet to be tested for monitoring highly motile cells, which are often biologically important. In this paper, we seek the conditions that enable effective and long-term trapping of a prominent model ciliate Tetrahymena thermophila within a hydrodynamic microfluidic device. Although motility and flexibility of T. thermophila make it difficult to avoid escaping from the trap, we show that tuning some key parameters in the hydrodynamic circuit was effective to achieve approximately 40 h cell retention, which is long enough to monitor cell behaviors over several generations. Here, we demonstrate the real-time observation of cell division and phagocytic digestion, revealing interesting phenomena such as a wide distribution in doubling time in a poor synthetic medium and heterogeneous time courses in digestion processes. Our results present a strategy for trapping highly motile ciliate cells in order to study the dynamic behaviors of single cells.

    DOI: 10.1039/c2lc40367f

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  • Micro-droplet model for recursive growth and division dynamics of the cell Reviewed

    T. Ichii, H. Suzuki, T. Yomo

    EPL   96 ( 4 )   2011.11

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    Living cells replicate by passing through cycles of growth and division, and they act as containers for chemical reactions. To determine basic properties of such recursively growing and dividing micro-compartments, we constructed a model system in a micro-fluidic device. In this system, droplets in a water-in-oil emulsion underwent cycles of fusion and subsequent division with nutrient-mimicking droplets. We found that repeating the combinations of growth (by fusion) and division of the droplets brought about their steady size distribution that had a skewed shape, similar to that which had been observed for proliferating cells. Our numerical analysis suggested that the skewness was originated from stochasticity in the addition of volumes in the growth process. Copyright (C) EPLA, 2011

    DOI: 10.1209/0295-5075/96/48006

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  • Programmed vesicle fusion triggers gene expression Reviewed

    F. Caschera

    Langmuir   27 ( 21 )   13082 - 13090   2011.9

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  • Micro-droplet system that models the recursive growth and division of the cell volumes Reviewed

    T. Ichii, H. Suzuki, T. Yomo

    Europhysics Letters   96   2011.3

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  • Microfluidic lipid membrane formation on microchamber arrays Reviewed

    Sadao Ota, Hiroaki Suzuki, Shoji Takeuchi

    LAB ON A CHIP   11 ( 15 )   2485 - 2487   2011

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    We present a simple method to form free-standing lipid membranes on arrayed microchambers (> 100). The formed membranes are perpendicular to an imaging plane with control of solute concentration on each side of the membranes. This platform let us quantitatively detect membrane transport of non-charged fluorescent molecules, induced by membrane proteins.

    DOI: 10.1039/c1lc20334g

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  • Detection of association and fusion of giant vesicles using a fluorescence-activated cell sorter Reviewed

    T. Sunami

    Langmuir   26 ( 19 )   15098 - 15103   2010.9

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  • Cellular compartment model for exploring the effect of the lipidic membrane on the kinetics of encapsulated biochemical reactions Reviewed

    T. Sunami

    Langmuir   26 ( 11 )   8544 - 8551   2010.2

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  • Synthesis of functional proteins within liposomes. Reviewed

    Sunami T, Matsuura T, Suzuki H, Yomo T

    Methods in molecular biology (Clifton, N.J.)   607   243 - 256   2010

  • Multichannel Simultaneous Measurements of Single-Molecule Translocation in alpha-Hemolysin Nanopore Array Reviewed

    Toshihisa Osaki, Hiroaki Suzuki, Bruno Le Pioufle, Shoji Takeuchi

    ANALYTICAL CHEMISTRY   81 ( 24 )   9866 - 9870   2009.12

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    We present a microarray system that enables simultaneous monitoring of multiple ionic currents through transmembrane a-hemolysin nanopores arrayed at bilayer lipid membranes. We applied the self assembling ability of lipid molecules interfaced between an aqueous solution and organic solvent to induce bilayer membrane formation at a microfluidic device; the device consists of a hydrophobic polymer film that serves to suspend the lipid-containing solvent at micrometer-sized apertures as well as to separate the aqueous solution into two chambers. In this study, we confirmed that expeditious and reproducible bilayer formation is realized by control of the composition of the solvent, a mixture of n-decane and 1-hexanol, which permits simultaneous incorporation of the a-hemolysin nanopores to the membrane array. Monitoring the eight wells on the array at once, we obtained a maximum of four relevant, synchronous signals of translocating ionic current through the nanopores. The system was also able to detect translocation events of nucleic acid molecules through the pore via the profile of a blocked current, promising its potential for high-throughput applications.

    DOI: 10.1021/ac901732z

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  • Population analysis of structural properties of giant liposomes by flow cytometry Reviewed

    Kazuya Nishimura, Tomohiro Hosoi, Takeshi Sunami, Taro Toyota, Masanori Fujinami, Koichi Oguma, Tomoaki Matsuura, Hiroaki Suzuki, Tetsuya Yomo

    Langmuir   25   10439 - 10443   2009.8

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    DOI: 10.1021/la902237y

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  • Ninety-six-well planar lipid bilayer chip for ion channel recording Fabricated by hybrid stereolithography Reviewed

    Hiroaki Suzuki, Bruno Le Pioufle, Shoji Takeuhci

    BIOMEDICAL MICRODEVICES   11 ( 1 )   17 - 22   2009.2

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    We present a micro fluidic chip for parallel ion channel recording in a large array of artificial planar lipid bilayer membranes. To realize a composite structure that features an array of recording wells with free-standing microapertures for lipid bilayer reconstitution, the device was fabricated by the hybrid stereolithography technology, in which a Parylene film with pre-formed microapertures was inserted during the rapid stereolithography process. We designed and tested a hybrid chip that has 96 (12x8) addressable recording wells to demonstrate recording of ion channel current in high-throughput manner. Measurement was done by sequentially moving the recording electrode, and, as a result, the channel current of model membrane protein was detected in 44 wells out of 96. We also showed that this hybrid fabrication process was capable of integrating micropatterned electrodes suitable for automated recording. These results support the efficiency of our present architecture of the parallel ion channel recording chip toward realization of the high-throughput screening of ion channel proteins in the artificial lipid bilayer system.

    DOI: 10.1007/s10544-008-9205-4

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  • Quantitative study of the structure of multilamellar giant liposomes as a container of protein synthesis reaction Reviewed

    K. Hosoda

    Langmuir   24   13540 - 13548   2008.10

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  • Lipid bilayer microarray for parallel recording of transmembrane ion currents Reviewed

    Bruno Le Pioufle, Hiroaki Suzuki, Kazuhito V. Tabata, Hiroyuki Noji, Shoji Takeuchi

    ANALYTICAL CHEMISTRY   80 ( 1 )   328 - 332   2008.1

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    This paper describes a multiwell biochip for simultaneous parallel recording of ion current through transmembrane pores reconstituted in planar lipid bilayer arrays. Use of a thin poly(p-xylylene) (parylene) film having micrometersized apertures (phi = 15-50 mu m, t = 20 mu m) led to formation of highly stable bilayer lipid membranes (BLMs) for incorporation of transmembrane pores; thus, a large number of BLMs could be arrayed without any skillful technique. We optically confirmed the simultaneous formation of BLMs in a 5 x 5 matrix, and in our durability test, the BLM lasted more than 15 h. Simultaneous parallel recording of alamethicin and gramicidin transmembrane pores in multiple contiguous recording sites demonstrated the feasibility of high-throughput screening of transmembrane ion currents in artificial lipid bilayers.

    DOI: 10.1021/ac7016635

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  • Microfluidic formation of lipid bilayer array for membrane transport analysis Reviewed

    Sadao Ota, Wei-Heong Tan, Hiroaki Suzuki, Shoji Takeuchi

    MEMS 2008: 21ST IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, TECHNICAL DIGEST   18 - +   2008

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    We present a highly parallel and reproducible method for reconstituting an array of lipid bilayers to analyze membrane transport. We infuse buffer/lipid/buffer solutions sequentially into a microchannel with numerous microchambers in its walls and seal each chamber by a lipid bilayer containing membrane proteins. Due to the small volume of the chamber (2 pL), membrane transport of confined fluorescent molecules across the bilayer through the proteins is readily observed as changes in fluorescent intensity. We successfully perform quantitative measurement of the transport flux of fluorescent molecules (Calcein) through a-Hemolysin antibiotic pores.

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  • Formation of giant vesiclelike compartments from a planar lipid membrane by a pulsed jet Reviewed

    K. Funakoshi, H. Suzuki, S. Takeuchi

    Journal of the American Chemical Society   129   12608 - 12609   2007.4

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  • Electrophysiological recordings of single ion channels in planar lipid bilayers using a polymethyl methacrylate microfluidic chip Reviewed

    Hiroaki Suzuki, Kazuhito V. Tabata, Hiroyuki Noji, Shoji Takeuchi

    BIOSENSORS & BIOELECTRONICS   22 ( 6 )   1111 - 1115   2007.1

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    Planar lipid bilayers are used for functional studies of ion channel proteins using electrophysiological techniques. We have been developing a plastic micro-fluidic device for the reconstitution of planar lipid bilayers and electrophysiological recordings toward a "membrane protein chip" for high-throughput screening. In the previous report [Suzuki, H., Tabata, K.V., Noji, H., Takeuchi, S., 2006. Highly reproducible method of planar lipid bilayer reconstitution in polymethyl methacrylate microfluidic chip. Langmuir 22 (4), 1937-1942], we presented the method and device in which the reproducibility of planar lipid bilayers reached 90%, and multiple bilayers were formed simultaneously. In this communication, we show that our device has excellent electric properties suitable for ion channel analysis down to single molecular level. Additional aspects on the optical accessibility and controllability on lipid bilayer formation are also presented. (c) 2006 Published by Elsevier B.V.

    DOI: 10.1016/j.bios.2006.04.013

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  • Lipid bilayer formation by contacting monolayers in a microfluidic device for membrane protein analysis Reviewed

    K. Funakoshi, H. Suzuki, S. Takeuchi

    Analytical Chemistry   78   8169 - 8174   2006.10

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  • Highly-reproducible method for planar lipid bilayer reconstitution using a micro fluidic chip Reviewed

    H. Suzuki, K. Tabata, H. Noji, S. Takeuch

    Langmuir   22 ( 4 )   1937 - 1942   2006.1

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    DOI: 10.1021/la052534p

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  • Planar lipid bilayer reconstitution with a micro-fluidic system. International journal

    Hiroaki Suzuki, Kazuhito Tabata, Yasuyuki Kato-Yamada, Hiroyuki Noji, Shoji Takeuchi

    Lab on a chip   4 ( 5 )   502 - 5   2004.10

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    A planar lipid bilayer which is widely used for the electrophysiological study of membrane proteins in laboratories is reconstituted using a micro-fluidic system, in a manner that is suitable for automated processing. We fabricated micro-channels on both sides of the substrate, which are connected through a 100-200 microm aperture, and showed that the bilayer can be formed at the aperture by flowing the lipid solution and buffer, alternately. Parylene coating is found to be suitable for both bilayer formation and electric noise reduction. Future applications include a high-sensitivity ion sensor chip and a high-throughput drug screening device.

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  • A chaotic mixer for magnetic bead-based micro cell sorter Reviewed

    H Suzuki, CM Ho, N Kasagi

    JOURNAL OF MICROELECTROMECHANICAL SYSTEMS   13 ( 5 )   779 - 790   2004.10

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    An efficient magnetic force driven mixer with simple configuration is designed, fabricated, and tested. It is designed to facilitate the mixing of magnetic beads and biomolecules in a microchannel, where mixing is unavoidably inefficient due to its low Reynolds number. With appropriate temporal variation of the force field, chaotic mixing is achieved, hence the mixing becomes effective. The mixing device consists of embedded microconductors as a magnetic field source and a microchannel that guides the streams of working fluid. It is demonstrated that a pair of integrated micro conductors provides a local magnetic field strong enough to attract nearby magnetic beads. Mixing of magnetic beads is accomplished by applying a time-dependent control signal to a row of conductors, at the Reynolds number of as low as 10(-2). Two-dimensional numerical simulation has been performed to design the configuration of the channel and electrodes, which creates chaotic motion of beads. It is found that a simple two-dimensional serpentine channel geometry with the transverse electrodes is able to create the stretching and folding of material lines, which is a manifestation of chaos. The mixing pattern predicted by the simulation has been confirmed by both flow visualization and PTV (Particle Tracking Velocimetry) in the chaotic mixer fabricated, which should greatly increase the attachment of beads onto the target biomolecules. The optimum frequency of applied control signal is searched by evaluating the Lyapunov exponent in both numerical, and experimental particle tracking. It is found that the range of optimum Strouthal number is 5 < St < 12, which is defined by the channel width and the mean velocity.

    DOI: 10.1109/JMEMS.2004.835775

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  • Active control of an axisymmetric jet with distributed electromagnetic flap actuators Reviewed

    H. Suzuki, N. Kasagi, Y. Suzuki

    Experiments in Fluids   36   498 - 509   2004.1

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  • Planar lipid bilayer reconstitution with a micro-fluidic system Reviewed

    H Suzuki, K Tabata, Y Kato-Yamada, H Noji, S Takeuchi

    LAB ON A CHIP   4 ( 5 )   502 - 505   2004

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    A planar lipid bilayer which is widely used for the electrophysiological study of membrane proteins in laboratories is reconstituted using a micro-fluidic system, in a manner that is suitable for automated processing. We fabricated micro-channels on both sides of the substrate, which are connected through a 100-200 mm aperture, and showed that the bilayer can be formed at the aperture by flowing the lipid solution and buffer, alternately. Parylene coating is found to be suitable for both bilayer formation and electric noise reduction. Future applications include a high-sensitivity ion sensor chip and a high-throughput drug screening device.

    DOI: 10.1039/b405967k

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  • 磁性粒子を利用したカオス的マイクロ混合器 Reviewed

    鈴木宏明, 笠木伸英,Ho, C. M

    日本機械学会論文集B編   69B ( 688 )   2626 - 2632   2003.2

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  • Planar lipid membrane array chip fabricated by MEMS technology

    Suzuki H., Noji H., Takeuchi S.

    Seibutsu Butsuri   43   S118   2003

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    DOI: 10.2142/biophys.43.S118_2

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  • Pneumatic Microballoons for Active Control of the Vibration-Induced Flow Reviewed

    Taku Sato, Kanji Kaneko, Takeshi Hayakawa, Hiroaki Suzuki

    Micromachines   14 ( 11 )   2010 - 2010   2023.10

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    Vibration-induced flow (VIF), in which a mean flow is induced around a microstructure by applying periodic vibrations, is increasingly used as an active flow-control technique at the microscale. In this study, we have developed a microdevice that actively controls the VIF patterns using elastic membrane protrusions (microballoons) actuated by pneumatic pressure. This device enables on-demand spatial and temporal fluid manipulation using a single device that cannot be achieved using a conventional fixed-structure arrangement. We successfully demonstrated that the device achieved displacements of up to 38 µm using the device within a pressure range of 0 to 30 kPa, indicating the suitability of the device for microfluidic applications. Using this active microballoon array, we demonstrated that the device can actively manipulate the flow field and induce swirling flows. Furthermore, we achieved selective actuation of the microballoon using this system. By applying air pressure from a multi-input channel system through a connection tube, the microballoons corresponding to each air channel can be selectively actuated. This enabled precise control of the flow field and periodic switching of the flow patterns using a single chip. In summary, the proposed microdevice provides active control of VIF patterns and has potential applications in advanced microfluidics, such as fluid mixing and particle manipulation.

    DOI: 10.3390/mi14112010

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  • Patterning-Based Self-Assembly of Specific and Functional Structures Invited Reviewed

    Taichi Kokubu, Tatsuya Hikida, Hiroaki Suzuki

    Journal of Robotics and Mechatronics   35 ( 5 )   1219 - 1226   2023.10

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    In this study, we developed a system for selective self-assembly of millimeter-scale components differentiated by adhesive patterns. This was achieved by designing concentric circular patterns having different radii but the same total length of peripheries. Small polymer sheets having solder adhesive patterns in these designs were simply attached to the millimeter-scale components to be assembled in a stirring container. This strategy was effective in avoiding an overlap between different patterns and enforcing the selective bonds between identical patterns among three types of components. Finally, the selective assembly of a functional structure (i.e., poly(N-isopropylacrylamide) gel actuator) was demonstrated.

    DOI: 10.20965/jrm.2023.p1219

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  • Formation of topological defects at liquid/liquid crystal interfaces in micro-wells controlled by surfactants and light Reviewed

    Kenji Katayama, Takuro Yoshimura, Saki Yamashita, Hiroto Teratani, Tomoki Murakami, Hiroaki Suzuki, Jun-ichi Fukuda

    Soft Matter   19 ( 34 )   6578 - 6588   2023

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    Harnessing liquid crystals to control topological defects. Our innovative method empowers precise manipulation of liquid crystal topological defects, offering a platform for homogeneous isolated topological and light-driven control.

    DOI: 10.1039/d3sm00838j

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  • Development of a Microchamber Device for Local Evaluation of Epithelial Cell Barrier Function and Cellular Imaging Reviewed

    R. Kida, M. Tsugane, H. Suzuki

    Proc. MHS 2022 (33rd Int. Symp. Micro-NanoMechatronics and Human Science)   2022.11

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  • Detection and Quantification of Nanoparticles Using the Vibration-Induced Flow Reviewed

    K. Kaneko, M. Tsugane, T. Sato, Y. Hasegawa, T. Hayakawa, H. Suzuki

    Proc. MHS 2022 (33rd Int. Symp. Micro-NanoMechatronics and Human Science)   2022.11

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  • Single-cell trapping in open microfluidics

    T. Murakami, H. Suzuki

    Proc. μTAS 2022   2022.10

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  • Concentration-controlled generation of DNA condensates within monodisperse giant unilamellar vesicles Reviewed

    R. Yoneyama, R. Ushiyama, T. Maruyama, M. Takinoue, H. Suzuki

    Proc. μTAS 2022   2022.10

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  • Optimization of the cell-free protein synthesis in monodisperse liposomes produced by microfluidics Reviewed

    R. Ushiyama, R. Sato, M. Tsugane, T. Matsuura, H. Suzuki

    Proc. μTAS 2022   2022.10

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  • Numerical study of the vibration-induced chaotic mixer based on vibration switching Reviewed

    K. Kaneko, Y. Hasegawa, T. Hayakawa, Hiroaki Suzuki

    Proc. μTAS 2022   2022.10

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  • A self-assembling system using air-water interfacial tension as a bonding force Reviewed

    A. Ito, H. Suzuki

    Proc. μTAS 2022   2022.10

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  • Active control of the vibration-induced flow by pneumatically actuated micropillars Reviewed

    T. Sato, K. Kaneko, T. Hayakawa, H. Suzuki

    Proc. μTAS 2022   2022.10

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  • Numerical characterization of the vibration-induced flow in various conditions Reviewed

    Z. Huang, K. Kaneko, Y. Asada, Y. Hasegawa, T. Hayakawa, H. Suzuki

    Proc. μTAS 2022   2022.10

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  • Detection of nanoparticles in a minute sample using the vibration induced flow

    Kanji Kaneko, Mamiko Tsugane, Taku Sato, Takeshi Hayakawa, Yosuke Hasegawa, Hiroaki Suzuki

    2022 IEEE 17th International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)   2022.4

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    DOI: 10.1109/nems54180.2022.9791090

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  • Numerical simulation of vibration-induced mixer using a cylindrical pillar with various vibration directions Reviewed

    K. Kaneko, T. Hayakawa, Y. Hasegawa, H. Suzuki

    Proc. APCOT 2022   2022.4

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  • Electrofusion device with the structural trapping for massive and quantitative measurement of vesicle fusion Reviewed

    T. Okita, M. Tsugane, K. Shinohara, H. Suzuki

    Pacifichem 2021   2021.12

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  • Numerical simulation of microfluidics-based nanoparticle capture utilizing the vibration-induced flow

    K. Kaneko, N. Ujikawa, Y. Hasegawa, T. Hayakawa, H. Suzuki

    Pacifichem 2021   2021.12

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  • Size purification of giant unilamellar vesicles using the mesh filter with the high opening ratio Reviewed

    K. Shinohara, T. Okita, M. Tsugane, H. Suzuki

    Pacifichem 2021   2021.12

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  • Selective self-assembly of miniature mechanical components by the patterned low melting point metal Reviewed

    T. Kokubu, H. Suzuki

    Pacifichem 2021   2021.12

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  • Evaluation of the capturing efficiency nanoparticles in the vibration-induced micromixer Reviewed

    T. Sato, K. Kaneko, T. Okano, Y. Hasegawa, T. Hayakawa, H. Suzuki

    Pacifichem 2021   2021.12

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  • Production of monodisperse giant unilamellar vesicles using a microfluidic channel Reviewed

    R. Ushiyama, T. Matsuura, K. Koiwai, H. Suzuki

    Pacifichem 2021   2021.12

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  • Controlled formation of topological defects of liquid crystals in micro-wells Reviewed

    H. Sakanoue, S. Yamashita, T. Murakami, H. Suzuki, K. Katayama

    Liquid Crystals   49 ( 4 )   580 - 588   2021.11

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    DOI: 10.1080/02678292.2021.1991016

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  • Evaluation of the Capturing Efficiency of Exosome in a Micromixer Driven by the Vibration-Induced Flow Reviewed

    K. Kaneko, M. Tsugane, T. Sato, T. Hayakawa, Y. Hasegawa, H. Suzuki

    Proc. μTAS 2021   2021.10

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  • Numerical and Experimental Analysis of the Vibration-Induced Flow around Complex Pillar Shapes Reviewed

    T. Sato, H. Zhitai, N. Ujikawa, K. Kaneko, Y. Hasegawa, T. Hayakawa, H. Suzuki

    Proc. μTAS 2021   2021.10

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  • Directional Control of Electronic Components by Pattern Complementarity in the Self-Assembling System Reviewed

    K. Nakayama, T. Hikida, H. Suzuki

    Proc. μTAS 2021   2021.10

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  • Monodispersion of Giant Unilamellar Vesicles Using a Metal Mesh Filter Reviewed

    K. Shinohara, T. Okita, M. Tsugane, T. Kondo, H. Suzuki

    Proc. μTAS 2021   2021.10

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  • A Microfluidic Device with Silicon Electrodes for Quantitative Evaluation of Vesicle Fusion Reviewed

    T. Okita, M. Tsugane, K. Shinohara, K. Kato, H. Suzuki

    Proc. μTAS 2021   2021.10

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  • A Numerical Simulation of Pumpless-Chaotic Micromixer Utilizing the Vibration-Induced Flow Reviewed

    K. Kaneko, N. Ujikawa, Y. Hasegawa, T. Hayakawa, H. Suzuki

    Acoustofluidics 2021   2021.8

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  • Usefulness of cell-penetrating peptides and penetration accelerating sequence for nose-to-brain delivery of glucagon-like peptide-2 Reviewed

    T. Akita, R. Kimura, S. Akaguma, M. Nagai, Y. Nakao, M. Tsugane, H. Suzuki, J. Oka, C. Yamashita

    Journal of Controlled Release   335 ( 10 )   575 - 583   2021.6

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    DOI: 10.1016/j.jconrel.2021.06.007

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  • Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins Reviewed

    R. E. Razan, N. Chanthra, T. Anzai, K. Koiwai, T. Murakami, H. Suzuki, Y. Hanazono, H. Uosaki

    J. Vis. Exp.   ( 169 )   e62129   2021.3

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    DOI: 10.3791/62129

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  • Efficient Production of Monodisperse Giant Unilamellar Vesicles by Transferring across the W-O Interface Reviewed

    R. Ushiyama, H. Suzuki

    Proc. MEMS 2021   2021.1

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  • Battery-free Built-in Micropump Driven by a Self-Propelled Droplet Reviewed

    T. Okano, K. Otsubo, J. Wada, H. Suzuki

    Proc. μTAS 2020   2020.10

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  • A Microchamber Device for Evaluation of the Barrier Functions of Epithelial Cells Reviewed

    M. Nakano, M. Tsugane, D. Sakuma, F. Sunaga, H. Suzuki

    Proc. MEMS 2020   2020.1

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  • Ejection of Large Particulate Materials from Giant Unilamellar Vesicles

    Shota Katsuta, Taiji Okano, Hiroaki Suzuki

    Proc. μTAS 2019   2019.10

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  • Evaluation of Mixing Performance of On-chip Micromixer with Low Dead Volume Based on Vibration-Induced Flow Reviewed

    T. Matsui, H. Suzuki, T. Hayakawa

    Proc. μTAS 2019   2019.10

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  • Templated self-assembly of microcomponents using water-oil interface Reviewed

    Ryo Hamano, Hiroaki Suzuki

    Proc. Transducers 2019   2019.6

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  • A Pumpless Mixer for Efficient Capturing of Small Particles Utilizing Vibration-Induced Flow Reviewed

    Kanji Kaneko, Taiji Okano, Takeshi Hayakawa, Yosuke Hasegawa, Hiroaki Suzuki

    Proc. MEMS 2019   406 - 408   2019.1

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  • Entropy-Driven Self-Assembly of Mesoscale Three-Dimensional Objects Reviewed

    R. Kawai, Y. Mori, H. Suzuki

    Proc. μTAS 2018   37 - 39   2018.10

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  • Towards Developing a “Droplet Motor” Driven by the Belousov-Zhabotinski Reaction: Control of Self-Propelled Motion Using a Ratchet Microchannel Reviewed

    T. Okano, K. Otsubo, J. Wada, H. Suzuki

    Proc. µTAS 2018   337 - 339   2018.10

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  • A Numerical Model for Three-Dimensional Analysis of Vibration-Induced Flow Reviewed

    K. Kaneko, T. Osawa, Y. Kametani, Y. Hasegawa, H. Suzuki

    Proc. µTAS 2018   1   465 - 427   2018.10

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    Copyright© (2018) by Chemical and Biological Microsystems Society.All rights reserved. A mean flow is induced around a micro-pillar by applying a small circulating vibration (typically < 10 μm amplitude and 100-1000 Hz frequency) in parallel to the whole substrate (Figure 1). This technique has been applied for cell manipulation and mixing biochemical samples with no external pump. We constructed a numerical model to analyze a three-dimensional flow field induced by the oscillating micro-pillar. A flow profile obtained by simulation agreed well with that of PIV measurement. The present numerical model can be easily extended to three-dimensional flow fields with various pillar designs and arrangements.

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  • High-Resolution Imaging of the Vertical Section of Adherent Cells Using a Microfluidic Device Reviewed

    M. Nakano, S. Araki, M. Tsugane, F. Sunaga, H. Suzuki

    Proc. µTAS 2018   928 - 929   2018.10

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  • Shape Study of Giant Liposomes Containing Macromolecules as an Artificial Cell Mimic Reviewed

    T. Okano, K. Inoue, H. Suzuki

    Proc. IEEE NEMS 2018   2018.4

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  • Reagent Handling and Delivery System Using Cell-Sized Liposomes Reviewed

    S.Katsuta, T. Okano, H. Suzuki

    Proc. IEEE NEMS 2017   2018.4

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  • Fracture characterization of inhomogeneous wrinkled metallic films deposited on soft substrates Reviewed

    Hiroshi Kishida, Satoshi Ishizaka, Takumi Nagakura, Hiroaki Suzuki, Akio Yonezu

    Journal of Physics D: Applied Physics   50 ( 49 )   495301   2017.11

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    This study investigated the fracture properties of wrinkled metallic films on a polydimethylsiloxane (PDMS) soft substrate. In particular, the crack density of the wrinkled film during tensile deformation was examined. In order to achieve better deformability of metallic thin films, a method to fabricate a wrinkled thin film on a PDMS soft substrate was first established. The copper (Cu) nano-film fabricated in this study possessed a wrinkled geometry, which plays a critical role in determining the extent of large elastic deformation. To create the wrinkled structure, wet-etching with a polymeric sacrificial layer was used. A sacrificial layer was first deposited onto a silicone rubber sheet. During the curing process of the layer, a compressive strain was applied such that the hardened surface layer buckled, and a wrinkled form was obtained. Subsequently, a PDMS solution was used to cover the layer in order to form a wrinkled PDMS substrate. Finally, the Cu film was deposited onto the wrinkled PDMS, such that the wrinkled Cu film on a soft PDMS substrate was fabricated. The use of uni-axial tensile tests resulted in film crack generation at the stress concentration zone in the wrinkled structure of the films. When the tensile loading was increased, the number of cracks increased. It was found that the increase in crack density was strongly related to the inhomogeneous nature of the wrinkled structure. Such a trend in crack density was investigated using FEM (finite element method) computations, such that this study established a simple mechanical model that may be used to predict the increase in crack density during tensile deformation. This model was verified through several experiments using various wrinkle patterns. The proposed mechanical model may be useful to predict the crack density of a wrinkled metallic film subject to tensile loading.

    DOI: 10.1088/1361-6463/aa95cb

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  • Design of pumpless chaotic mixing device driven by the vibration-induced flow Reviewed

    K. Kaneko, T. Osawa, Y. Kametani, Y. Hasegawa, H. Suzuki

    Proc. microTAS 2017   255 - 256   2017.10

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    © 17CBMS-0001. Mixing of minute reagents is one of the common operational elements in integrated microfluidic devices. Although a variety of mixer units have been developed, most of them are designed to use external pumps. In this study, we designed a pumpless micro-mixer for mixing reagents in μL volume. We employed a vibration-induced flow, in which a mean circulating flow around a micro-pillar is induced by giving small circulating vibration to the whole substrate. By employing non-axisymmetric micro-pillar design and switching of circulating direction, we show that stretching and folding of the fluid element, a feature of chaotic advection, can be induced.

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  • Microfluidic device for selective manipulation of giant liposomes Reviewed

    T. Okano, H. Suzuki

    Proc. microTAS 2017   1567 - 1568   2017.10

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  • Liposome-based RNA detection system Reviewed

    M. Tsugane, H. Suzuki

    Proc. microTAS 2017   1615 - 1616   2017.10

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  • The selective binding technique using hydrophilic/hydrophobic patterning for self-assembly Reviewed

    T. Okuyama, T. Okano, H. Suzuki

    Proc. microTAS 2017   323 - 324   2017.10

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  • Characterization of microchamber device for efflux assay of adherent cell Reviewed

    T. Eda, M. Tsugane, Y. Okada, H. Suzuki

    Proc. MicroTAS 2016   2079 - 2080   2016.10

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  • One-Step PDMS Micromolding Process for Tapered Three-Dimensional Structures Reviewed

    H. Suzuki, K. Mitsuno, K. Shiroguchi, T. Okano, T. Dohi, T. Tsuji

    Proc. APCOT 2016   2016.6

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  • SELF-ASSEMBLY OF MULTI-COMPONENT MICROSTRUCTURE USING THE ENTROPIC EFFECT Reviewed

    Ushio Okabe, Taiji Okano, Hiroaki Suzuki

    2016 IEEE 29TH INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS)   465 - 468   2016

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    We have been testing the application of the entropic effect (the depletion volume effect) for the self-assembly of microcomponents. Microcomponents with the arbitrary shapes fabricated by the negative photoresist were immersed and gently agitated in the solution. When the solution contains nanometric macromolecule at relatively high concentration, they formed assembled structures, to increase the translational entropy of macromolecules in the solution. In the report, we attempted the assembly of the desired structure composed of multiple components, and found that it is important to design the accessible complementary shape with the greater interfacial area to render the specific bonding effectively.

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  • Optimization of the Entropic Self-assembly of Microcomponents Reviewed

    U. Okabe, T. Okano, H. Suzuki

    Proc. Micro TAS 2015   1416 - 1418   2015.10

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  • 3D-shaped microchamber for the Single-cell Analysis Reviewed

    K. Mitsuno, H. Ikeda, M. Tsugane, T. Okano, K. Shiroguchi, H. Suzuki

    Proc. Micro TAS 2015   522 - 524   2015.10

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  • Liposome-based Liquid Handling for Biochemical Reactions Reviewed

    T. Okano, H. Suzuki, T. Yomo

    Proc. Micro TAS 2015   2032 - 2034   2015.10

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  • Detection of Transport Activity of Culture Cells Using Microchamber Device Reviewed

    M. Tsugane, H. Suzuki

    Proc. Micro TAS 2015   2079 - 2080   2015.10

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  • Liquid Handling of Minute Volume Using the Hydrogel Encapsulating Liposomes Reviewed

    K. Takahashi, T. Okano, H. Suzuki

    Proc. Micro TAS 2015   478 - 480   2015.10

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  • 3-D Self-assembly Using the Hydrophilic/hydrophobic Patterning Reviewed

    K. Kimura, T. Okano, H. Suzuki

    Proc. ISMM 2015   2015.6

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  • Polymer-based Disposable Accelerometer Reviewed

    D. Takahashi, T. Okano, H. Suzuki

    Proc. ISMM 2015   2015.5

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  • A MICROWELL DEVICE FOR MEASUREMENT OF MEMBRANE TRANSPORT OF ADHERENT CELLS Reviewed

    Y. Okada, M. Tsugane, H. Suzuki

    2015 28TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2015)   439 - 442   2015

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    We developed the microwell device for measurement of membrane transport of single adherent cells. As the cells in a population (e.g., tumor) is inevitably heterogeneous, a technique to measure the transport activities at a single-cell level is needed. When adherent cells were cultured on the microwells with similar to 10 mu m diameter, they spread over the opening to form the closed picoliter space. Thus, molecules exported from cells accumulate in such a space and be detected by fluorescence imaging. In this report, we show that, by employing horizontal microwell design, materials exported from the cell membrane can be visualized without overlapping with the cell, increasing the S/N ratio of the fluorescence signal. Efflux of the cancer drug transported by the multidrug resistance protein was detected.

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  • SELF-ASSEMBLY OF MICROCOMPONENTS USING THE ENTROPIC EFFECT Reviewed

    U. Okabe, T. Okano, H. Suzuki

    2015 28TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS (MEMS 2015)   300 - 303   2015

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    We propose the use of the entropic effect (the depletion volume effect), which is at work in the assembly of biomolecules, for the self-assembly of artificially engineered microcomponents. When the solution contains macromolecule at relatively high concentration, microcomponents formed assembled structures. The bonding energy is not originated from the surface; it is generated by increasing the translational entropy of macromolecules in the solution. We expect that use of the depletion volume effect promotes the search of the global free-energy minimum in the system by avoiding being trapped to the local minima in the self-assembly process.

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  • Reaction control by stirring-induced, discrete, recursive fution and division of femtoliter compartments in emulsion Reviewed

    T. Ichii, G. Tanahashi, H. Suzuki, T. Yomo

    Proc. µTAS 2013   1233 - 1235   2013.10

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  • Manipulation of liposome-based bioreactor featuring adding, mixing, and aliquoting femtoliter volumes Reviewed

    H. Shiomi, S. Tsuda, H. Suzuki, T. Yomo

    Proc. microTAS 2013   1048 - 1050   2013.10

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  • 2P263 Directed evolution of a self-encoding system(20. Origin of life & Evolution,Poster)

    Sunami Takeshi, Ichihashi Norikazu, Nishikawa Takehiro, Kazuta Yasuaki, Matsuura Tomoaki, Suzuki Hiroaki, Yomo Tetsuya

    Seibutsu Butsuri   53 ( 1 )   S202   2013

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    DOI: 10.2142/biophys.53.S202_4

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  • Coupling of the fusion and budding of giant phospholipid vesicles containing macromolecules Reviewed

    Hiroaki Suzuki, Hidetoshi Terasawa, Kazuya Nishimura, Tomoaki Matsuura, Tetsuya Yomo

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   244   2012.8

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  • Statistical analysis of the discrete encapsulation of nanomaterials in colloidal capsules Reviewed

    Tatsuya Sakakura, Kazuya Nishimura, Hiroaki Suzuki, Tetsuya Yomo

    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY   244   2012.8

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  • Shrunk to femtolitre: Tuning high-throughput monodisperse water-in-oil droplet arrays for ultra-small micro-reactors Reviewed

    Tianzhun Wu, Katsuki Hirata, Hiroaki Suzuki, Rong Xiang, Zikang Tang, Tetsuya Yomo

    APPLIED PHYSICS LETTERS   101 ( 7 )   2012.8

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    We report a facile, low-cost, and high-yielding microfluidic technology for in situ generating and arraying water-in-oil droplets by shrinking them to the order of femtolitres (fLs) as scalable batch micro-reactors. Instead of generating ultra-small droplets by the direct atomization, which requires dedicate control and high energy input, we shrink droplets to stable smaller ones by utilizing the controlled water diffusion in oil. This "shrunk to fL" method is combined with a three-dimensional microwell design to create high-density addressable droplet arrays. As the result, scalable, high-throughput, and well-aligned W/O arrays with excellent long-term stability and predicable droplet sizes have been achieved. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4746754]

    DOI: 10.1063/1.4746754

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  • Statistical Analysis of Liposome Budding Dynamics Based on Free Energy Landscape Reviewed

    S. Tsuda, H. Suzuki, T. Yomo

    13th Int. Conf. Simulation & Synthesis of Living Systems (Afile 13)   623 - 624   2012.6

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  • Creating an artificial cell with different size revealed the effect of compartment volume on the intracompartmental multimeric protein synthesis Reviewed

    T. Matsuura, K. Hosoda, H. Suzuki, T. Yomo

    13th Int. Conf. Simulation & Synthesis of Living Systems (Afile 13)   619 - 620   2012.6

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  • Importance of Parasite RNA Species Repression for Prolonged Translation-Coupled RNA Self-Replication Reviewed

    Yohsuke Bansho, Norikazu Ichihashi, Yasuaki Kazuta, Tomoaki Matsuura, Hiroaki Suzuki, Tetsuya Yomo

    CHEMISTRY & BIOLOGY   19 ( 4 )   478 - 487   2012.4

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    Increasingly complex reactions are being constructed by bottom-up approaches with the aim of developing an artificial cell. We have been engaged in the construction of a translation-coupled replication system of genetic information from RNA and a reconstituted translation system. Here a mathematical model was established to gain a quantitative understanding of the complex reaction network. The sensitivity analysis predicted that the limiting factor for the present replication reaction was the appearance of parasitic replicators. We then confirmed experimentally that repression of such parasitic replicators by compartmentalization of the reaction in water-in-oil emulsions improved the duration of self-replication. We also found that the main source of the parasite was genomic RNA, probably by nonhomologous recombination. This result provided experimental evidence for the importance of parasite repression for the development of long-lasting genome replication systems.

    DOI: 10.1016/j.chembiol.2012.01.019

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  • 2PT205 Directed evolution of a self-encoding system using giant liposome(The 50th Annual Meeting of the Biophysical Society of Japan)

    Sunami Takeshi, Ichihashi Norikazu, Nishikawa Takehiro, Kazuta Yasuaki, Matsuura Tomoaki, Suzuki Hiroaki, Yomo Tetsuya

    Seibutsu Butsuri   52   S139   2012

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    DOI: 10.2142/biophys.52.S139_5

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  • The permeability properties of the model protocell

    N. Yamanaka

    Int. Symp. Synthesizing Life & Biol. Syst.   2011.10

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  • Reconstructing of gene expression in micro compartments

    K. Hirata

    17th Int. Biophys. Cong. (IUPAB) & 12th Natl. Biophys. Cong.   2011.10

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  • Single cell observation of Tetrahymena thermophila using microfluidic devices

    I. Kumano

    Int. Symp. Synthesizing Life & Biol. Syst.   2011.10

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  • Construction of a glass microchamber for cell-free protein synthesis

    T. Okano

    Int. Symp. Synthesizing Life & Biol. Syst.   2011.10

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  • Analyzing the noise of cell-free gene expression using cell-sized liposome

    K. Nishimura, S. Tsuru, H. Suzuki, T. Yomo

    Int. Symp. Synthesizing Life & Biol. Syst.   2011.10

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  • Monodisperse femtoliter water-in-oil droplet array for compartmentalizing artificial cell

    T. Wu

    17th Int. Biophys. Cong. (IUPAB) & 12th Natl. Biophys. Cong.   2011.10

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  • Statistical analysis of discrete encapsulation in giant vesicles

    T. Sakakura, K. Nishimura, H. Suzuki, T. Yomo

    Int. Symp. Synthesizing Life & Biol. Syst.   2011.10

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  • Directed evolution of a self-encoding system using giant liposome

    T. Sunami

    Int. Symp. Synthesizing Life & Biol. Syst.   2011.10

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  • The evolvability of a self-encoding system under different number of gene copies

    K. Uno

    Int. Symp. Synthesizing Life & Biol. Syst.   2011.10

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  • Coupling fusion and budding of giant liposomes

    H. Terasawa

    Int. Symp. Synthesizing Life & Biol. Syst.   2011.10

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  • Fusion-division dynamics of emulsion droplets under stirring as a model for the cell growth

    G. Tanahashi, T. Ichii, H. Suzuki, T. Yomo

    Int. Symp. Synthesizing Life & Biol. Syst.   2011.10

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  • Micro compartment array for studying the gene expression in cell-sized volumes

    K. Hirata

    Int. Symp. Synthesizing Life & Biol. Syst.   2011.10

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  • Giant unilamellar vesicles as a platform of liquid handling in femtoliter volumes Reviewed

    H. Terasawa

    Proc. MicroTAS 2011   1618 - 1620   2011.10

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  • Tunable monodisperse femtoliter drolet array using 3D microfluidic traps Reviewed

    T. Wu

    Proc. MicroTAS 2011   1659 - 1661   2011.10

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  • Analysis of the effect of compartment size on internal multimeric protein synthesis reactions

    T. Matsuura

    Int. Symp. Synthesizing Life & Biol. Syst.   2011.10

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  • Statistical description of liposome shape transformation dynamics based on free energy landscape

    S. Tsuda, H. Suzuki, T. Yomo

    Int. Symp. Synthesizing Life & Biol. Syst.   2011.10

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  • Skewed size distribution observed in recursive water-in-oil droplets

    T. Ichii, H. Suzuki, T. Yomo

    Int. Symp. Synthesizing Life & Biol. Syst.   2011.9

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  • Origin of lognormal-like distributions with a common width in a growth and division process Reviewed

    Kazufumi Hosoda, Tomoaki Matsuura, Hiroaki Suzuki, Tetsuya Yomo

    PHYSICAL REVIEW E   83 ( 3 )   2011.3

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    Lognormal statistical distributions are observed in a variety of scientific fields. The widths of these distributions in the log scale are often similar, but the underlying mechanism that maintains these widths within a small range has not been well explained. We show that a stochastic process of halving followed by addition can yield a stationary distribution that resembles the universal lognormal distribution with a certain width. The mechanism that we propose here would provide insight into the essence of why lognormal-like distributions in many systems have a common width.

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  • Evolvability and self-replication of genetic information in Liposomes Reviewed

    Tomoaki Matsuura, Norikazu Ichihashi, Takeshi Sunami, Hiroshi Kita, Hiroaki Suzuki, Tetsuya Yomo

    The Minimal Cell: The Biophysics of Cell Compartment and the Origin of Cell Functionality   275 - 287   2011

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    To realize the minimal cell by a bottom-up approach, increasingly -complex biochemical reactions are being encapsulated in lipid vesicles (liposomes). Here, we describe the encapsulation of one of the general properties of living organisms into liposomes, i.e., replication of genetic information with self-encoded replicase, and evolvability. We also discuss the possibility of realizing a minimal cell that can -proliferate autonomously. © 2011 Springer Science+Business Media B.V.

    DOI: 10.1007/978-90-481-9944-0_15

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  • 2F1624 Mere existence of growth/division yields cell-like size-homeostasis(Mathematical biology 2,The 48th Annual Meeting of the Biophysical Society of Japan)

    Hosoda Kazufumi, Matsuura Tomoaki, Suzuki Hiroaki, Yomo Tetsuya

    Seibutsu Butsuri   51   S84   2011

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    DOI: 10.2142/biophys.51.S84_4

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  • Bio-inspired 3D self-patterning of functional coatings for PDMS microfluidics Reviewed

    Tianzhun Wu, Hiroaki Suzuki, Tetsuya Yomo

    2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11   2311 - 2314   2011

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    We report a novel, simple and effective 3D self-patterning method for functional coatings on poly(dimethylsiloxane) (PDMS) microfluidics before bonding, inspired by the wettability-based fast evaporation of water film on superhydrophilic plant leaves. Induced by evaporation, liquid-phase functional coatings are selectively deposited on rough PDMS surfaces, enabling the conventional PDMS-glass bonding. Several control factors are investigated to improve the coating quality including roughness, coverage, uniformity and clogging issues. Teflon-like CYTOP coatings have been demonstrated on PDMS channels with good coverage, uniformity and bonding strength, and significantly reduced the swelling, deformation and collapse of PDMS channels in toluene. © 2011 IEEE.

    DOI: 10.1109/TRANSDUCERS.2011.5969541

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  • In-situ generation and shrinkage of monodisperse water-in-oil emulsion for femtoliter compartmentalization using capillary traps Reviewed

    Tianzhun Wu, Hiroaki Suzuki, Tetsuya Yomo

    2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11   1765 - 1768   2011

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    A novel, facile and effective microfluidic approach to achieve monodisperse femtoliter (fL, 1015L) water-in-oil (W/O) droplet array is proposed for constructing artificial cell-sized micro reactors. By combining the controlled diffusion of water into oil and capillary-based 2D micro trap array (capillary traps), in-situ generation and 1000-fold volume shrinkage down to ∼4 fL have been achieved for monodisperse W/O array. With improved 3D trap array, high-density and well-aligned fL W/O droplet array with good long-time stability and coefficient of variation (CV) smaller than 5% has also been demonstrated. This approach also enables dynamically tuning of droplet sizes and solution concentration useful for many applications. © 2011 IEEE.

    DOI: 10.1109/TRANSDUCERS.2011.5969434

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  • Measurement of nonlinear biochemical reaction in microdroplets using the fractal-shaped micro channel Reviewed

    K. Hirata

    Proc. MicroTAS 2010   623 - 625   2010.10

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  • Amplification of RNA in growing and dividing micro-droplets Reviewed

    T. Ichii, H. Suzuki, T. Yomo

    Proc. MicroTAS 2010   2089 - 2091   2010.10

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  • Shrunk to nano: A novel approach for femtoliter compartmentalization using W/O emulsion Reviewed

    T. Wu, H. Suzuki, T. Yomo

    Proc. MicroTAS 2010   437 - 439   2010.10

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  • Measurement of nonlinear biochemical reaction in microdroplets using the fractal-shaped micro channel Reviewed

    K. Hirata

    Proc. MicroTAS 2010   1712 - 1714   2010.10

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  • Constructing Partial Models of Cells Reviewed

    Norikazu Ichihashi, Tomoaki Matsuura, Hiroshi Kita, Takeshi Sunami, Hiroaki Suzuki, Tetsuya Yomo

    COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY   2 ( 6 )   a004945   2010.6

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    Understanding the origin of life requires knowledge not only of the origin of biological molecules such as amino acids, nucleotides and their polymers, but also the manner in which those molecules are integrated into the organized systems that characterize cellular life. In this article, we introduce a constructive approach to understand how biological molecules can be arranged to achieve a higher-order biological function: replication of genetic information.

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  • 2P346 A basic understanding for cell size homeostasis(The 48th Annual Meeting of the Biophysical Society of Japan)

    Hosoda Kazufumi, Matsuura Tomoaki, Suzuki Hiroaki, Yomo Tetsuya

    Seibutsu Butsuri   50 ( 2 )   S143 - S144   2010

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    DOI: 10.2142/biophys.50.S143_5

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  • 2P241 Budding transition of giant unilamellar vesicles after electro-fusion(The 48th Annual Meeting of the Biophysical Society of Japan)

    Terasawa Hidetoshi, Nishimura Kazuya, Suzuki Hiroaki, Matsuura Tomoaki, Yomo Tetsuya

    Seibutsu Butsuri   50 ( 2 )   S125   2010

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    DOI: 10.2142/biophys.50.S125_2

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  • 2P250 Detection of association and fusion of giant vesicles using fluorescence-activated cell sorter(The 48th Annual Meeting of the Biophysical Society of Japan)

    Sunami Takeshi, Caschera Filippo, Morita Yuuki, Toyota Taro, Nishimura Kazuya, Matsuura Tomoaki, Suzuki Hiroaki, Hanczyc Martin M., Yomo Tetsuya

    Seibutsu Butsuri   50 ( 2 )   S126 - S127   2010

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    DOI: 10.2142/biophys.50.S126_6

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  • 1P070 Co-translational folding of beta-galactosidase and beta-glucuronidase in an in vitro translation system(Protein:Property,The 48th Annual Meeting of the Biophysical Society of Japan)

    Matsuura Tomoaki, Hosoda Kazufumi, Ichihashi Norikazu, Kazuta Yasuaki, Suzuki Hiroaki, Yomo Tetsuya

    Seibutsu Butsuri   50 ( 2 )   S31   2010

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    DOI: 10.2142/biophys.50.S31_4

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  • Electro-optical imaging microscopy of dye-doped artificial lipidic membranes Reviewed

    B. Haji

    Biophysical Journal   97   2913 - 2921   2009.11

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  • Droplet-based continuous cell culture system by repeating fusion-division cycles Reviewed

    T. Ichii, H. Suzuki, T. Yomo

    Proc. MicroTAS 2009   558 - 560   2009.10

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  • Fractal-shaped micro channel system for kinetic analysis of biochemical reaction Reviewed

    K. Hirata, T. Ichii, H. Suzuki, T. Yomo

    Proc. MicroTAS 2009   1001 - 1003   2009.10

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  • Size control of unilamellar giant vesicles using microfluidics Reviewed

    K. Nishimura, T. Toyota, H. Suzuki, T. Yomo

    Proc. MicroTAS 2009   1291 - 1293   2009.10

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  • DETECTION AND ANALYSIS OF PROTEIN SYNTHESIS AND RNA REPLICATION IN GIANT LIPOSOMES Reviewed

    Takeshi Sunami, Hiroshi Kita, Kazufumi Hosoda, Tomoaki Matsuura, Hiroaki Suzuki, Tetsuya Yomo

    METHODS IN ENZYMOLOGY; LIPOSOMES, PT F   464   19 - 30   2009

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    In living cells, biochemical reaction systems are enclosed in small lipidic compartments. To experimentally simulate various biochemical reactions occurring in extant cells, giant liposomes are used to reconstruct an artificial model cell. We present methods for conducting a protein synthesis reaction, followed by the reaction catalyzed by the synthesized proteins inside liposomes, and for measurement of the in liposome reaction using a fluorescence-activated cell sorter (FACS). These techniques enable us to perform detailed analysis of the biochemical reactions occurring in the microcompartments, and have the potential to reveal the role of compartmentalization in cellular systems.

    DOI: 10.1016/S0076-6879(09)64002-7

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  • 1P-254 Fractal-shaped micro channel system for kinetic analysis of biochemical reaction(Measurements, The 47th Annual Meeting of the Biophysical Society of Japan)

    Hirata Katsuki, Ichii Tetsuo, Suzuki Hiroaki, MaTsuura Tomoaki, Yomo Tetsuya

    Seibutsu Butsuri   49   S102   2009

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    DOI: 10.2142/biophys.49.S102_2

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  • 1P-184 Quantitative evaluation of biochemical reactions encapsulated in giant unilamellar liposomes(Biol & Artifi memb.:Structure & Property, The 47th Annual Meeting of the Biophysical Society of Japan)

    Nishimura Koji, Sunami Takeshi, Suzuki Hiroaki, Matsuura Tomoaki, Yomo Tetsuya

    Seibutsu Butsuri   49   S91   2009

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    DOI: 10.2142/biophys.49.S91_2

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  • Erratum: Ninety-six-well planar lipid bilayer chip for ion channel recording fabricated by hybrid stereolithography (Biomedical Microdevices 10.1007/s10544-008-9205-4) Reviewed

    Hiroaki Suzuki, Bruno Le Pioufle, Shoji Takeuchi

    Biomedical Microdevices   11 ( 1 )   23   2009

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    DOI: 10.1007/s10544-008-9218-z

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  • Size control of unilamellar giant vesicles using microfluidics

    K. Nishimura, T. Toyota, H. Suzuki, T. Yomo

    Int. Sympo. Complex Systems Biology   2009

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  • Electro-Optical Imaging Microscopy of Dye Doped Lipid Bilayer Reviewed

    Bassam Hajj, Sophie De Reguardati, Loic Hugonin, Toshihisa Osaki, Hiroaki Suzuki, Shoji Takeuchi, Bruno Le Pioufle, Dominique Chauvat, Joseph Zyss

    2009 CONFERENCE ON LASERS AND ELECTRO-OPTICS AND QUANTUM ELECTRONICS AND LASER SCIENCE CONFERENCE (CLEO/QELS 2009), VOLS 1-5   3 - +   2009

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    We report the first imaging of a dye-doped artificial bilayer using electro-optical microscopy. The applied voltage dependence confirms the origin of the effect. This result is an important step towards a contactless optical patch-clamp.

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  • Analysis of volume and lipid membrane quantity of giant liposomes using Fluorescence Activated Cell Sorter

    K. Nishimura

    11th Liposome Research Days Conference   19 - 22   2008.6

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  • 2S8-6 Dynamics of structure and internal reactions in liposomes explored by fluorescence-activated cell sorter(2S8 Giant Liposome Research Front Line,The 46th Annual Meeting of the Biophysical Society of Japan)

    Suzuki Hiroaki, Sunami Takeshi, Hosoda Kazufumi, Matsuura Tomoaki, Yomo Tetsuya

    Seibutsu Butsuri   48   S13   2008

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    DOI: 10.2142/biophys.48.S13_6

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  • イオンチャネルスクリーニングのための平面脂質二重膜マイクロアレイチップ Reviewed

    鈴木宏明

    生産と技術   60 ( 4 )   66 - 69   2008

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  • Control of fusion and division of W/O emulsion droplets as a model of cell propagation

    T. Ichii, H. Suzuki, T. Yomo

    IUMRS Int. Conf. in Asia   2008

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  • Platform for controlling micro-emulsions as a model of growth and division cycle of the cell

    T. Ichii, H. Suzuki, T. Yomo

    17th CDB meeting towards synthesis of cells   2008

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  • 3P-277 Platform for controlling micro-emulsions as a model of growth and division cycle of the cell(The 46th Annual Meeting of the Biophysical Society of Japan)

    Ichii Tetsuo, Suzuki Hiroaki, Yomo Tetsuya

    Seibutsu Butsuri   48   S170   2008

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    DOI: 10.2142/biophys.48.S170_4

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  • 3P-208 Transport Property of Polynucleotide Molecules on α-Hemolysin Nanopore Array(The 46th Annual Meeting of the Biophysical Society of Japan)

    Osaki Toshihisa, Suzuki Hiroaki, Pioufle Bruno Le, Takeuchi Shoji

    Seibutsu Butsuri   48   S159   2008

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    DOI: 10.2142/biophys.48.S159_6

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  • 3P-275 Quantitative analysis of interactions between the phospholipid membrane and encapsulated reaction systems in cell-sized liposomes(The 46th Annual Meeting of the Biophysical Society of Japan)

    Sunami Takeshi, Hosoda Kazufumi, Kita Hiroshi, Ichihashi Norikazu, Matsuura Tomoaki, Suzuki Hiroaki, Yomo Tetsuya

    Seibutsu Butsuri   48   S170   2008

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    DOI: 10.2142/biophys.48.S170_2

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  • Highly parallelized lipidic bilayers array for ion channel recording Reviewed

    B. Le Pioufle, H. Suzuki, S. Takeuchi

    Proc. MicroTAS 2007   679 - 681   2007.10

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  • 3-D micro fluidic chip for membrane protein analysis Reviewed

    H. Suzuki, S. Takeuchi

    Proc. MicroTAS 2007   1276 - 1278   2007.10

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  • A parylene lift-off process with microfluidic channels for selective protein patterning Reviewed

    K. Atsuta, H. Suzuki, S. Takeuchi

    Journal of Micromechanics and Microengineering   17   496 - 500   2007.2

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  • Supported lipid bilayer array to study clathrin mediated endocytosis in vitro Reviewed

    Hiroaki Suzuki, Thomas Pucadyil, Rajesh Ramachandran, Shoji Takeuchi, Sandra L. Schmid

    PROCEEDINGS OF THE IEEE TWENTIETH ANNUAL INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, VOLS 1 AND 2   466 - +   2007

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    We describe our effort to realize an assay system to study clathrin-mediated endocytosis (CME) in vitro. Since CME related proteins are known to bind phosphatidylinositol-4,5-bisphosphate (PI(4,5)P-2), we propose an accessible method to create an array of supported lipid bilayers (SLBs) with different lipid compositions, using a PDMS stamp and a microchannel. Formation of a uniform SLB of a complex lipid mixture containing PI(4,5)P-2 was confirmed by fluorescent imaging and fluorescence recovery after photobleaching (FRAP). Our technique should allow us to vary the concentration of PI(4,5)P-2 or other membrane constituents to study their requirements. In preliminary experiments we showed that dynamin binds to the planar bilayer containing PI(4,5)P-2, but without causing membrane tubulation as it does when dynamin binds liposomes.

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  • 2P242 Planar Lipid Bilayer Array for Parallel Ion Channel Recording(Native and artificial biomembranes-structure and properties,Poster Presentations)

    Suzuki Hiroaki, Pioufle Bruno Le, Takeuchi Shoji

    Seibutsu Butsuri   47   S173   2007

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    DOI: 10.2142/biophys.47.S173_3

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  • Formation of ultra giant vesicles from a planar lipid membrane

    K. Funakoshi, H. Suzuki, S. Takeuchi

    5th East Asian Biphys. Sympo. & 44th Annu. Meet. Biophy. Soc. Japan (EABS & BSJ)   2006.11

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  • Membrane transport assay system for transporter proteins using artificial lipid bilayers

    H. Suzuki, K. Tabata, H. Noji, S. Takeuchi

    5th East Asian Biphys. Sympo. & 44th Annu. Meet. Biophy. Soc. Japan (EABS & BSJ)   2006.11

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  • Low cost microfabricated silicon chip for membrane protein monitoring

    B. L. Pioufle

    Proc. MicroTAS 200   1393 - 1395   2006.11

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  • Giant proteoliposome array in microchambers Reviewed

    N. Yamanaka

    Proc. MicroTAS 2006   1354 - 1356   2006.11

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    This paper describes two novelties which will contribute to the precise examination of the functions of membrane proteins. First, we describe a method to reconstitute purified membrane proteins in giant liposomes (giant proteoliposomes). Second, we established a system to observe the chemical/physical responses of liposomes by confining them in microchamber arrays during the perfusion of surrounding solution through a semi-permeable membrane. Since the position of proteoliposomes does not change during perfusion, we can continuously observe the response of single liposomes. Using this technique, we successfully detected the function of water channel, humanaquaporin1 (hAQP1), by observing rapid shrinkage of its proteoliposomes due to the change in osmotic gradient. © 2006 Society for Chemistry and Micro-Nano Systems.

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  • Development of an assay system for ABC transporters reconstituted in an artificial lipid bilayer Reviewed

    H. Suzuki, K. V. Tabata, H. Noji, S. Takeuchi

    Proc. MicroTAS 2006   1363 - 1365   2006.11

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  • Blowing vesicles: A simple method for direct microencapsulation in lipid vesicles Reviewed

    K. Funakoshi, H. Suzuki, S. Takeuchi

    Proc. MicroTAS 2006   534 - 536   2006.11

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  • Biomolecular linear motors confined to move upon micro-patterns on glass Reviewed

    Y. Yoshida

    Journal of Micromechanics and Microengineering   16 ( 8 )   1550 - 1554   2006.6

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  • Formation of lipid vesicles from a planar lipid bilayer using pulsed jet flow

    K. Fuankoshi, H. Suzuki, S. Takeuchi

    Proc. Int. Conf. Microtechnologies in Medicine and Biology   68 - 69   2006.5

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  • Ultra giant vesicles out of a planar membrane Reviewed

    Kei Funakoshi, Hiroaki Suzuki, Shoji Takeuchi

    2006 INTERNATIONAL CONFERENCE ON MICROTECHNOLOGIES IN MEDICINE AND BIOLOGY   68 - +   2006

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    We report a method for forming ultra giant lipid vesicles. A chip device with a micro-jet nozzle was fabricated. The vesicles were produced by applying pulsed micro jets to a planar lipid bilayer, analogous to blowing soap bubbles from a liquid film. Using this method, lipid vesicles with diameters of about 500 mu m were formed one-by-one. This method enables the encapsulation of target molecules or nano particles into the vesicles rapidly and efficiently.

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  • Characterization of the membrane transport assay system using microchamber array

    H. Suzuki, K. V. Tabata, H. Noji, S. Takeuchi

    2006 INTERNATIONAL CONFERENCE ON MICROTECHNOLOGIES IN MEDICINE AND BIOLOGY   301 - +   2006

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    We have been developing a measurement system for membrane transport across a reconstituted planar lipid bilayer to examine the functions of transporter membrane proteins using a microchamber array (Membrane Microchamber System). In this system, an artificial planar lipid bilayer is pressed on the Parylene microchamber array (typically 0.1 similar to 1 pL in volume) fabricated on a coverglass to enclose the volume. After membrane proteins are reconstituted in the bilayer, fluorescently labeled molecules transported across the bilayer are accumulated in microchambers, and detected by fluorescent imaging. The microchamber array is compatible with high-sensitive biological imaging techniques, such as a confocal microscopy and a total internal reflection fluorescence microscopy (TIRFM). Due to the tiny volume of microchambers, concentration of the transported molecules rapidly increases. In this study, the detection system was characterized in detail. With the aid of microchambers, the concentration reached to the detectable level with as small as 10(2)similar to 10(3) fluorescent molecules.

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  • Lipid bilayer microchambers: An optical detection system for membrane transport Reviewed

    H Suzuki, KV Tabata, H Noj, S Takeuchi

    MEMS 2006: 19TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, TECHNICAL DIGEST   482 - 485   2006

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    We present a novel method for the optical detection of membrane transport across an artificial planar lipid bilayer through membrane proteins (e.g., ion pumps and transporters) using a microchamber array. Parylene microchambers are fabricated on a coverglass, using photolithography, and a planar lipid bilayer is pressed on the microchambers to cover. After membrane proteins are reconstituted in the bilayer, fluorescently labeled molecules transported through them are accumulated in microchambers and detected by fluorescent imaging. The microchamber array is shown to be compatible with high-sensitive microscopic imaging techniques, such as a confocal microscopy and a total internal reflection fluorescence microscopy (TIRFM). Using this system, transport of fluorescent dye through peptide nanopore (alpha-hemolysin) is examined.

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  • Lipid bilayer formation by contacting monolayers Reviewed

    K. Funakoshi, H. Suzuki, S. Takeuchi

    Proc. MicroTAS 2005   2005   951 - 953   2005.10

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  • Simultaneous reconstitution of multiple planar lipid bilayers Reviewed

    H. Suzuki, K. Tabata, H. Noji, S. Takeuchi

    Proc. MicroTAS 2005   1300 - 1302   2005.10

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  • 3P321 High reproducible method for multiple array of artificial planar lipid bilayers

    Suzuki H., Tabata K., Noji H., Takeuchi S.

    Seibutsu Butsuri   45   S284   2005

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    DOI: 10.2142/biophys.45.S284_1

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  • Planar lipid bilayer chip for electrophysiological analysis of membrane proteins Reviewed

    H Suzuki, K Tabata, Y Kato-Yamada, H Noji, S Takeuchi

    MICRO TOTAL ANALYSIS SYSTEMS 2004, VOL 2   ( 297 )   246 - 248   2005

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    Planar bilayer membranes have been widely used for electrophysiological analysis of membrane proteins in laboratories. We previously reported that planar lipid bilayer can be reconstituted at 100 similar to 150 mu m tapered hole fabricated in a silicon-based micro-fluidic channel system. However, for the measurement of ion channel current, which requires pico-ampere sensitivity, the substrate material should be non-conductive material to eliminate the electric noise. Thus, we fabricated PMMA plastic based micro-fluidic system made by precise machining. The activity of channel proteins incorporated into the planar membrane is successfully recorded.

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  • Fine patterning of protein with parylene sheet Reviewed

    K Atsuta, H Suzuki, S Takeuchi

    Micro Total Analysis Systems 2004, Vol 2   ( 297 )   249 - 251   2005

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    We propose a fine protein patterning technique using a patterned Parylene film on a substrate. This method prevents non-specific binding, and can be performed in the wet condition to avoid drying of proteins. FITC and IC3-PE-maleimid labeled bovine albumin are successfully patterned in 20 x 20 mu m(2) spots with 2 mu m intervals. Patterning of different materials on different spots becomes possible by introducing sample solution through PDMS micro fluidic channel place on top. This technique will easily be applied to the analysis of protein in an array. We have demonstrated a selective patterning of beads with different fluorescence in 3 x 3 array (80 x 80 mu m(2) spots with 50 mu m intervals).

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  • Selective protein patterning in microfluidic channels using a Parylene lift-off process Reviewed

    K Atsuta, H Suzuki, S Takeuchi

    Transducers '05, Digest of Technical Papers, Vols 1 and 2   1584 - 1587   2005

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    This paper describes a method for patterning proteins selectively using a combination of microfluidics and a lift-off process. Microchannels are used to introduce different protein solutions, allowing us to immobilize spots of proteins on a substrate. We fabricated microfluidic channels in polymethylmethacrylate (PMMA) substrate and deposited a thin Parylene film as a mask containing small holes patterned on the PMMA layer. Solutions of proteins were introduced in p channels and immobilized on the PMMA surface at positions defined by the parylene mask. The parylene mask was lifted-off, leaving different proteins and/or nanoparticles patterned on a PMMA substrate. Using microfluidic channel, different kinds of samples were able to be delivered and patterned on a same substrate in the wet condition; this method avoids denaturing of proteins without non-specific binding.

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  • 1P227 A device of lipid bilayer formation for membrane transport imaging

    Funakoshi K., Suzuki H., Takeuchi S.

    Seibutsu Butsuri   45   S88   2005

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    DOI: 10.2142/biophys.45.S88_3

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  • Formation process of planar lipid bilayer observed by confocal microscopy

    H Suzuki, K Tabata, H Noji, S Takeuchi

    2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology   272 - 275   2005

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    In this paper, the formation process of planar lipid bilayer in PMMA micro-fluidic device is investigated in detail by observing distribution of fluorescent lipid molecules with the aid of confocal microscopy. In our bilayer formation process, initially thick layer (similar to 50Rm) of lipid solution, which is stuck at the vertical section of the aperture edge, gradually thins down by applying static pressure in the upper compartment. It finally becomes a bilayer with the pressure of 200-400Pa. It is confirmed by recording single channel current through gramicidin. The yield of bilayer formation reached 90% in maximum.

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  • Biomolecular linear motors confined to move upon micropatterns on glass Reviewed

    Y Yoshida, R Yokokawa, H Suzuki, K Atsuta, H Fujita, S Takeuchi

    MEMS 2005 MIAMI: TECHNICAL DIGEST   750 - 753   2005

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    Biomolecular linear motor proteins --kinesin and microtubules -- are finely patterned on a conventional, flat glass substrate using parylene lift-off process; these patterns are useful for single molecule analysis and biohybrid transportation. The patterning was performed from 5 mu m - 40 mu m in width with minimal non-specific binding of the proteins. A conventional gliding assay was realized using this glass substrate and the behaviors of the single proteins were analyzed.

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  • Planar lipid bilayer array chip using micro fluidic system

    H. Suzuki, K. Tabata, H. Noji, S. Takeuchi

    Proc. 21st Sensor Symposium   505 - 508   2004.10

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  • Planar lipid membrane array for membrane protein chip Reviewed

    H Suzuki, Y Kato-Yamada, H Noji, S Takeuchi

    MEMS 2004: 17TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, TECHNICAL DIGEST   272 - 275   2004

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    We report the novel methods to reconstitute artificial lipid membranes in an array on a chip, using the micro-fluidic system, for the batch analysis of membrane proteins. The micro-fabricated device should render the ease of injection/recirculation of the reagents as well as multi-channel and high-sensitive detection of protein activities incorporated in the bilayer membrane. It is shown that the lipid bilayer membrane can be formed on 100 similar to 200 mum square apertures fabricated in a silicon substrate in two methods. In the first method, the bilayer is formed by flowing the lipid solution and the buffer alternately. In the second method, it is done by placing a buffer droplet on a thin layer of the lipid solution. Parylene coating is found to be suitable for both bilayer formation and noise reduction. The future application includes the high-sensitive ion sensor chip and high-throughput drug screening chip.

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  • Design optimization of planar lipid bilayer chip

    H. Suzuki, K. Tabata, H. Noji, S. Takeuchi

    4th KIMM-EPFL-IIS Joint Workshop on Micro/Nano Mechatronics and Production Technologies   2004

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  • Planar lipid membrane array for membrane protein chip Reviewed

    H Suzuki, Y Kato-Yamada, H Noji, S Takeuchi

    MEMS 2004: 17TH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, TECHNICAL DIGEST   272 - 275   2004

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    We report the novel methods to reconstitute artificial lipid membranes in an array on a chip, using the micro-fluidic system, for the batch analysis of membrane proteins. The micro-fabricated device should render the ease of injection/recirculation of the reagents as well as multi-channel and high-sensitive detection of protein activities incorporated in the bilayer membrane. It is shown that the lipid bilayer membrane can be formed on 100 similar to 200 mum square apertures fabricated in a silicon substrate in two methods. In the first method, the bilayer is formed by flowing the lipid solution and the buffer alternately. In the second method, it is done by placing a buffer droplet on a thin layer of the lipid solution. Parylene coating is found to be suitable for both bilayer formation and noise reduction. The future application includes the high-sensitive ion sensor chip and high-throughput drug screening chip.

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  • Particle tracking velocimetry measurement of chaotic mixing in a micro-mixer

    H. Suzuki, M. Nakano, N. Kasagi, C. M. Ho

    Proc. Int. Symp. Micro-Mechanical Eng.   2003   397 - 402   2003.12

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    Mixing of magnetic beads and bio-molecules becomes critically important in a micro-scale cell sorting system. Particle tracking velocimetry (PTV) has been employed to examine the motion of magnetic beads in a model chaotic micro-mixer. The measured trajectories of beads show good agreement with the numerical simulation previously reported [5], which has predicted the onset of chaos. The bead trajectory is reconstructed from the phase-averaged velocity field of beads to estimate the Lyapunov exponent. The optimum frequency of applied control signal exists in the range of the Strouhal number of 5<St<12,which is also in accordance with the numerical simulation. The present method of estimating the Lypaunov can be used to determine the performance of the mixing device for micro particles and large molecules.

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  • Chaotic mixing of magnetic beads in micro cell separator Reviewed

    H. Suzuki, N. Kasagi, C. M. Ho

    Proc. 3rd Int. Symp. Turbulence and Shear Flow Phenomena   817 - 822   2003.9

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  • A magnetic force driven chaotic micro-mixer Reviewed

    H. Suzuki, C. M. Ho

    Proc. MEMS 2002   665 - 670   2002.1

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  • A Chaotic Micro-Mixer Based on Magnetic Beads

    SUZUKI Hiroaki, KASAGI Nobuhide, Ho Chih Ming

    The proceedings of the JSME annual meeting   2002   103 - 104   2002

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    An integrated magnetic micro-mixer, which consists of embedded micro-conductors and a micro-channel, is developed by using MEMS technology for the mixing of magnetic beads in bio-fluids. In the previous report, it was shown that the simple 2-D micro-conductors provided a magnetic field strong enough to attract the nearby magnetic beads. Numerical simulation is used to search for the channel and electrode design that can generate chaotic trajectories of beads. It is found that the serpentine channel geometry with the perpendicular electrode arrangement is able to create the stretching and folding of material lines, which is a sign of chaos. Similar patterns are observed experimentally in the chaotic mixing in the fabricated device.

    DOI: 10.1299/jsmemecjo.2002.6.0_103

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  • Active control of an axisymmetric jet with an intelligent nozzle Reviewed

    H. Suzuki, N. Kasagi, Y. Suzuki

    Proc. 1st Symp. Turbulent Shear Flow Phenomena   665 - 670   1999.9

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  • フラップ型電磁アクチュエータ群による軸対称噴流の能動制御 Reviewed

    鈴木宏明, 笠木 伸英, 鈴木 雄二

    日本機械学会論文集B編   65B ( 639 )   3644 - 3651   1999.5

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  • Manipulation of a round jet with electromagnetic flap actuators Reviewed

    H Suzuki, N Kasagi, Y Suzuki, H Shima

    MEMS '99: TWELFTH IEEE INTERNATIONAL CONFERENCE ON MICRO ELECTRO MECHANICAL SYSTEMS, TECHNICAL DIGEST   534 - 540   1999

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    A novel axisymmetric jet nozzle equipped with a row of miniature electromagnetic flap actuators on its circular lip is developed for active flow control. Each of the flaps fabricated by photolithography is independently driven according to a control signal supplied by a PC. The spatio-temporal flow structures of the controlled jet are studied through flow visualization and laser Doppler velocimetry (LDV) measurement. It is found that artificial disturbances generated by the flaps can modify the formation and evolution of large scale vortical structures significantly. When each half cluster of these flaps are driven out of phase, the jet clearly bifurcates into two branches and its mixing is markedly enhanced in the plane of bifurcation.

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Books

  • 細胞のメカニクス

    David Boal著, 鈴木宏明訳( Role: Sole translator)

    2020 

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    メカノバイオロジーや再生医療に欠かせない、「細胞を対象とした力学」の解説書。 本書では、細胞の物理的・力学的性質に重点をおき、細胞の基本的な構成要素(ソフト材料)から全体(複合材料)へと解説を進めている。実験と理論の両面から平易に説明し、生物系や物理系、工学系いずれの読者にとっても、応用に役立つ結果がよくわかるよう配慮がなされている。特に、近年研究が進んできた「ゆらぎ」に対する応用は、ほぼすべてのトピックを通じて扱っている。 重要性が増している学際的な分野について、幅広い知識が見通しよくまとめられた、充実の書籍である。

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  • Dividing Small Numbers: The Discreteness and Distribution of Molecules in the Cell Membrane": Chap 10 in T. Nagai and Y. Togashi Ed., "Minorities and Small Numbers from Molecules to Organisms in Biology"

    H. Suzuki( Role: Contributor)

    Springer  2018  ( ISBN:9789811320835

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  • Spying minority in biological phenomena

    ( Role: Sole author第10章 少数を分ける)

    日本評論社  2017.3 

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  • 人工細胞の創生とその応用

    ( Role: Joint author2章1 人工細胞容器としてのリポソーム|rn|岡野太治,鈴木宏明)

    シーエムシー出版  2017.1 

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  • Self-assembly The science of things that put themselves together

    ( Role: Sole translator)

    森北出版株式会社  2015.2 

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  • The Minimal Cell: The Biophysics of Cell Compartment and the Origin of Cell Functionality

    T. Matsuura, ほか( Role: Joint author)

    Springer  2011 

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  • Cold Spring Harber Perspectives in Biology

    N. Ichihashi ほか( Role: Joint author)

    Cold Spring Harbor Lab. Press  2011 

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  • 細胞を創る・生命システムを創る|rn|実験医学増刷

    角南武志( Role: Joint author)

    羊土社  2011 

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  • Methods in molecular Biology

    T. Sunami, T. Matsuura, H. Suzuki, T. Yomo( Role: Joint author)

    Springer  2010 

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  • Methods in Enzymology

    T. Sunami, ほ( Role: Joint author)

    Elsevier  2009 

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  • 機械工学 最近10年のあゆみ

    ( Role: Joint author)

    社団法人日本機械学会  2007 

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MISC

  • Substrate Supply to Biochemical Reactions in Sub-picoliter Volumes

    33   1 - 5   2016.10

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  • Study of Selective Binding Method Using Hydrophilic/hydrophobic Patterning for Self-assembly

    33   1 - 5   2016.10

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  • 1C33 Volume Dependence of Cell-free Protein Synthesis Using a Glass Microchamber

    OKANO Taiji, MATSUURA Tomoaki, SUZUKI Hiroaki, YOMO Tetsuya

    2014 ( 26 )   91 - 92   2014.1

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  • J212013 Measurement of the protein synthesis from a single copy of DNA in the microwell

    SHIOMI Hideaki, TSUDA Soichiro, SUZUKI Hiroaki, YOMO Tetsuya

    Mechanical Engineering Congress, Japan   2013   "J212013 - 1"-"J212013-4"   2013.9

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    We report the utilization of giant unilamellar vesicles (GUVs) as a platform of handling chemical and biochemical reagents in femtoliter volumes. GUVs with diameter 5 to 10 μm containing chemical reagents together with inert polymers were subjected to electric pulses for fusion (electrofusion). After mixing of reagents, the fused GUV spontaneously deformed to a budding shape, separating the mixed solution into multiple sub-volumes^<(1)>. Here we utilized the microfluidic channel and the optical tweezers to select GUVs in interest, make them in contact, and fuse them together for mixing and dispensing chemical reagents. We also show that, by lowering the ambient temperature close to the phase transition temperature T_m of the lipid used, daughter GUVs completely detached (fission). This process completes all features for liquid handing used in biochemistry at femtoliter volumes, which have been unattained with the previous technologies.

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  • J026031 Measurement of the protein synthesis from a single copy of DNA in the microwell

    OKANO Taiji, MATSUlfRA Tomoaki, KAZUTA Yasuaki, SUZUKI Hiroaki, YOMO Tetsuya

    Mechanical Engineering Congress, Japan   2013   "J026031 - 1"-"J026031-4"   2013.9

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    We fabricated quartz microchambers for performing protein synthesis using a reconstituted in vitro transcription-translation system. By using glass microchambers, the yield of the green fluorescent protein (GFP) synthesis was significantly improved compared to that obtained in the widely-used poly(dimethylsiloxane) (PDMS)microchambers. Using this device, we demonstrated that GFP can be synthesized from a single copy of DNA. Quantized and distinctive signals from proteins synthesized from 0, 1, or 2 copies of genes were obtained. The microchamber presented here can be used for not only studying the effects of compartment volume on protein synthesis but also the comprehensive analysis of complex biochemical reactions in cell-mimetic environments.

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  • Protein Synthesis from a Single-molecule of DNA in a Glass Microchamber

    OKANO Taiji, MATSUURA Tomoaki, KAZUTA Yasuaki, SUZUKI Hiroaki, YOMO Tetsuya

    2012 ( 8 )   1 - 6   2012.6

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  • エッセイ:物理からのおおまかな生物観

    2012

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  • 解説記事:マイクロ技術による人工脂質膜の再構築と機能計測

    2009

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  • Microtechnologies for membrane protein studies

    Hiroaki Suzuki, Shoji Takeuchi

    ANALYTICAL AND BIOANALYTICAL CHEMISTRY   391 ( 8 )   2695 - 2702   2008.8

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    Despite the rapid and enormous progress in biotechnologies, the biochemical analysis of membrane proteins is still a difficult task. The presence of the large hydrophobic region buried in the lipid bilayer membrane (transmembrane domain) makes it difficult to analyze membrane proteins in standard assays developed for water-soluble proteins. To handle membrane proteins, the lipid bilayer membrane may be used as a platform to sustain their functionalities. Relatively slow progress in developing micro total analysis systems (mu TAS) for membrane protein analysis directly reflects the difficulty of handling lipid membranes, which is a common problem in bulk measurement technologies. Nonetheless, researchers are continuing to develop efficient and sensitive analytical microsystems for the study of membrane proteins. Here, we review the latest developments, which enable detection of events caused by membrane proteins, such as ion channel current, membrane transport, and receptor/ligand interaction, by utilizing microfabricated structures. High-throughput and highly sensitive detection systems for membrane proteins are now becoming a realistic goal. Although most of these systems are still in the early stages of development, we believe this field will become one of the most important applications of mu TAS for pharmaceutical and clinical screenings as well as for basic biochemical research.

    DOI: 10.1007/s00216-008-1916-0

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  • Three-Dimensional Micro Fluidic Chip for Membrane Protein Analysis Fabricated by Stereolithography

    SUZUKI Hiroaki, TAKEUCHI Shoji

    2007 ( 7 )   1 - 4   2007.7

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  • Lipid bilayer membrane array for in vitro assay of clathrin mediated endocytosis

    SUZUKI Hiroaki, PUCADYIL Thomas, RAMACHANDRAN Rajesh, TAKEUCHI Shoji, SCHMID Sandra L.

    2006 ( 15 )   23 - 26   2006.10

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  • Vesicle Formation Using Micro Pulsed Jet

    FUNAKOSHI Kei, SUZUKI Hiroaki, TAKEUCHI Shoji

    2006 ( 15 )   19 - 22   2006.10

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  • Vesicle Formation Using Micro Pulsed Jet

    FUNAKOSHI Kei, SUZUKI Hiroaki, TAKEUCHI Shoji

    2006 ( 1 )   9 - 12   2006.5

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  • Membrane Micro Chamber Array System for Measurement of Biomembrane Transport

    SUZUKI Hiroaki, TABATA Kazuhito, NOJI Hiroyuki, TAKEUCHI Shoji

    2006 ( 1 )   51 - 54   2006.5

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  • ニュースレター記事:マイクロTASのためのマイクロ混合器

    2004.12

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  • Active Control of Axisymmetric Jet by Using an Array of Electro-Magnetic Flap Actuators

    SUZUKI Hiroaki, KASAGI Nobuhide, SUZUKI Yuji

    11   287 - 290   1999.10

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  • Structure of the Axisymmetric Jet Controlled by the Intelligent Nozzle

    SHIMA Hiroki, SUZUKI Hiroaki, KASAGI Nobuhide, SUZUKI Yuji

    Journal of the Visualization Society of Japan   18   217 - 220   1998.7

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Presentations

  • Microfluidic technologies for artificial cell studies

    H. Suzuki

    MNC 2022  2022.11 

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  • Artificial cell studies in microfluidics

    H. Suzuki

    APCOT 2022  2022.4 

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  • Giant liposome-based dynamic bioreactor

    H. Suzuki

    4th Asian Symposium for Analytical Sciences (ASAS)  2018.9 

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  • Spontaneous enveloping of genome-size DNA into lipid membrane

    H. Suzuki, M. Tsugane, F. Sunaga, T. Okano

    BSJ Annual Meeting  2018.9 

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  • ジャイアントリポソームを用いたRNA検出系

    津金麻実子, 鈴木宏明

    H30 生体コモンスペース研究会  2018.7 

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  • Unique deformation modes and material|rn|encapsulation of giant unilamellar vesicles|rn|encapsulating biomacromolecules

    H. Suzuki

    International Symposium on Artificial Cell Reactor Science and Technology  2018.4 

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  • リポソーム内DNA封入法

    先端的バイオ計測研究会  2017.3 

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  • Liposome Technologies: Toward creation of artificial cell mimics

    1st Sino-Japan Seminar on Micro/Nano Systems for Biomedical Applications  2017.3 

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  • 人工細胞膜システムの細胞らしい挙動

    生体界面研究会  2017.2 

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  • 人工細胞膜システムの細胞らしい挙動

    東京理科大学研究推進機構総合研究院 イメージングフロンティアセンターシンポジウム  2016.12 

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  • 三次元マイクロ光造形鋳型を用いた樹脂転写とその力学解析

    iJSME2016  2016.11 

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  • 細胞の界面をつくる・制御する

    日本表面化学会関東支部第5回セミナー 東京理科大学ウォーターフロンティアサイエンスシンポジウム  2016.11 

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  • 人工細胞系構築の試み

    鈴木,岡野

    平成28年度生理研研究会 生体界面研究会  2016.7 

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  • 機械(メカ)屋からみた細胞~細胞はつくれるか?~

    ナノ茶論~Nano-Salon in Kawasaki~  2016.7 

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  • Distribution of genome-size DNA in the dividing model cell membrane

    H. Suzuki

    Pacifichem 2015  2016.1 

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  • 細胞膜と人工細胞膜のイメージングのためのマイクロ技術

    鈴木

    平成27年度生理研研究会 生体界面研究会  2015.7 

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  • 1細胞解析に向けた3次元マイクロチャンバー

    鈴木,三野, 池田,津金, 岡野,城口

    電気学会BMS研究会  2015.5 

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  • エントロピーから生まれる力

    新学術領域「少数性生物学」・さきがけ「細胞機能の構成的な理解と制御」合同シンポジウム  2015.1 

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  • 磁性粒子を利用したカオス混合器

    公益社団法人新化学技術推進協会(JACI) 電子情報技術部会・マイクロナノシステムと材料・加工分科会講演会  2014.11 

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  • バイオチップにおける マイクロ三次元加工技術への期待

    日本材料学会関東支部会 「新たな機能性材料開発のための3次元微細構造物の作成技術と応用」  2013.12 

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  • 細胞を模擬したソフト材料・システムの開発とその可能性

    鈴木宏明

    日本光学会年会学術講演会  2011.11 

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  • Modeling growth and division of cells

    H. Suzuki, T. Yomo

    Int. Symp. on Synthesizing Life and Biological Systems  2011.10 

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  • 蛍光セルソータを用いたリポソーム構造および内部反応のダイナミクス計測

    鈴木宏明

    日本生物物理学会  2008.10 

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  • 人工脂質膜マイクロチャンバーを用いた生体膜輸送計測システム

    鈴木宏明, 竹内昌治

    第14回化学とマイクロ・ナノシステム研究会  2006 

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  • Chaotic mixing of magnetic beads in micro cell separator

    H. Suzuki, N. Kasagi, C. M. Ho

    3rd Int. Symp. Turbulence and Shear Flow Phenomena  2003.9 

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  • A magnetic force driven chaotic micro-mixer

    H. Suzuki, C. M. Ho

    MEMS 2002/IEEE  2002.1 

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  • Active control of an axisymmetric jet with an intelligent nozzle

    H. Suzuki, N. Kasagi, Y. Suzuki

    1st Symp. Turbulent Shear Flow Phenomena  1999.9 

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  • Manipulation of a round jet with electromagnetic flap actuators

    H. Suzuki, N. Kasagi, Y. Suzuki, H. Shima

    MEMS 19991/IEEE  1999.1 

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Awards

  • Best Paper Award in 2023 International Syposium on Micro-NanoMechatronics and Human Science (MHS2023)

    2023.11   IEEE   Development of an Imaging-based Method for Evaluating Local Epithelial Paracellular Barrier Function

    Ryuta Kida, Mamiko Tsugane, Hiroaki Suzuki

  • CM Ho best paper in Micro/Nanofluidics

    2022.4   IEEE NEMS 2022   Detection of Nanoparticles in A Minute Sample Using the Vibration Induced Flow

    Kanji Kaneko, Mamiko Tsugane, Taku Sato, Takeshi Hayakawa, Yosuke Hasegawa, Hiroaki Suzuki

  • 日本生物物理学会若手奨励賞

    2006.11   日本生物物理学会   Membrane transport assay system for transporter proteins using artificial lipid bilayers

  • 日本機械学会奨励賞

    2000.4   日本機械学会   噴流制御のためのミニチュア・アクチュエータ群を備えたインテリジェントノズル・システムの構築の研究

Research Projects

  • ワイドレンジ周波数機械振動を用いたマルチスケール微細操作システムの創製

    Grant number:22H01454  2022.4 - 2025.3

    日本学術振興会  科学研究費助成事業  基盤研究(B)  中央大学

    早川 健, 鈴木 宏明, 工藤 謙一

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    Grant amount: \17810000 ( Direct Cost: \13700000 、 Indirect Cost: \4110000 )

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  • DNA Nanoscale Modality

    Grant number:20H05935  2020.11 - 2025.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Transformative Research Areas (A)  Tokyo Institute of Technology

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    Grant amount: \127530000 ( Direct Cost: \98100000 、 Indirect Cost: \29430000 )

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  • モデルベース設計を基盤とした指向性進化による高効率細胞プロセス創製の確立と展開

    2019.6 - 2024.3

    基盤研究S 

    清水浩

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    Grant type:Competitive

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  • 脂質二重膜バイオリアクタ形成デバイスの開発とバイオマーカ検出技術への応用

    2019.4 - 2023.3

    基盤研究B 

    鈴木宏明

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    Grant type:Competitive

    Grant amount: \17160000

    申請者らは最近,GUV内でRT-PCRを行うことで10分子程度の特定のmRNAを検出する方法を開発した.さらに,total RNAを含むGUVとRT-PCR試薬を含むGUVを電気的に膜融合させて内封物質を混合させ,特定のmRNAを増幅・検出可能であることを示した.以上の研究実績に基づき,本申請では,均一なリポソームリアクタを製造し,その応用として膜融合により生体膜内封物質を検出する技術開発を行う.細胞やオルガネラ,またエクソソーム等の小胞も脂質二重膜より構成されているため,これらに含まれる核酸等の直接増幅・検出に応用する.

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  • 分子演算システムによる腫瘍由来核酸の高速パターン診断

    2019.4 - 2023.3

    基盤研究A 

    川野竜司

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    Grant type:Competitive

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  • 振動誘起流れを用いた細胞スフェロイドの大量生産・品質評価

    2019.4 - 2022.3

    基盤研究B 

    早川健

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    Grant type:Competitive

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  • 人工細胞リアクタ生成法の開発

    2018.10 - 2022.3

    武田科学振興財団ライフサイエンス研究助成  民間助成金 

    鈴木宏明

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \2000000

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  • 振動誘起流れを用いた微量・局所溶液混合

    2017.4 - 2019.3

    中央大学 

    鈴木宏明

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \2700000

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  • 極微量の溶液混合を促進するポンプレスマイクロミキサーの開発

    2017.4 - 2018.3

    東京応化科学技術財団  民間助成金 

    鈴木宏明

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount: \1000000

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  • リポソーム内DNA封入法

    2016.10 - 2018.3

    受託研究費 

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    Grant type:Competitive

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  • 膜とDNAが協同して増幅する人工細胞の構築

    2015.7 - 2018.3

    文部科学省  科学研究費補助金(日本学術振興会・文部科学省)-基盤研究(C) 

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    Grant type:Competitive

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  • 接着細胞の垂直面高解像度イメージング法の開発

    2015.4 - 2018.3

    文部科学省  科学研究費補助金(日本学術振興会・文部科学省)-挑戦的萌芽研究 

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    Grant type:Competitive

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  • 超並列・高密度1細胞遺伝子発現解析へ向けたマイクロデバイス開発

    2014.4 - 2016.3

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    Grant type:Competitive

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  • 臨床応用に向けたがん細胞薬剤排出スクリーニングチップの開発

    2014.4 - 2015.3

    中谷医工計測技術振興財団助成金  民間助成金 

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    Grant type:Competitive

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  • リポソームを利用した極微量溶液操作系の構築

    2012.4 - 2014.3

    文部科学省  科学研究費補助金(日本学術振興会・文部科学省)-若手研究(B) 

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    Grant type:Competitive

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  • 脂質二重膜マイクロチャンバアレイを用いた生体膜輸送計測システム

    2007.4 - 2009.3

    文部科学省  科学研究費補助金(日本学術振興会・文部科学省)-基盤研究(B) 

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    Grant type:Competitive

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  • マイクロチャンバーによるABCトランスポーター基質測定系の開発

    Grant number:18657041  2006 - 2007

    日本学術振興会  科学研究費助成事業  萌芽研究  東京工業大学

    横山 謙, 鈴木 宏明

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    Grant amount: \3400000 ( Direct Cost: \3400000 )

    本研究の目標は、微細加工技術(MEMS)で作成したマイクロチャンバー上の人工膜にABCトランスポーターを再構成し、その輸送活性をリアルタイムで測定する系を確立することである。本研究では、ABCトランスポーターを平面膜に組み込む必要があるが、好熱菌のABCトランスポーターの大腸菌を宿主とした発現系の構築が必要である。先年度においてATPase活性を保持したヘテロオリゴマータイプのABCトランスポーターの発現および平面膜への膜タンパク質であるV-ATPaseの再構成の確認には成功している。
    ABCトランスポーターの平面膜への再構成効率を評価するために、蛍光色素を導入した。蛍光色素ラベルによりABCトランスポーターのATPase活性が消失することはなかった。1分子蛍光観察により平面膜への組み込みを調べた。効率は悪いものの、平面膜上に タンパク質由来のものと思われる輝点を多数確認した。蛍光を持つ輸送基質の取り込みをATP存在下で調べているが、背景に比べて明確なチャンバー内の蛍光強度の増加は確認されていない。その原因の一つとしてチャンバー自体の自然蛍光があり、現在チャンバーの素材の検討を行っている。

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  • マイクロ・ナノ加工技術を用いた膜タンパクアレイチップ

    2005.4 - 2006.3

    財団法人新世代研究所研究助成  民間助成金 

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    Grant type:Competitive

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  • 生体分子ナノアクチュエータを利用したナノ構造のハンドリングシステム

    2004.4 - 2006.3

    文部科学省  科学研究費補助金(日本学術振興会・文部科学省)-挑戦的萌芽研究 

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    Grant type:Competitive

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  • マイクロ・ナノ加工技術による膜タンパク質アレイチップ

    2004.4 - 2006.3

    文部科学省  科学研究費補助金(日本学術振興会・文部科学省)-基盤研究(B) 

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  • Microfluidic Devices for the analysis of protein functions with monodisperse liposomes

    Grant number:17360111  2005 - 2006

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)  The University of Tokyo

    TAKEUCHI Shoji, SUZUKI Hiroaki, NOJI Hiroyuki

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    Grant amount: \15600000 ( Direct Cost: \15600000 )

    In this research, we have studied a device for the efficient production of monodisperse liposomes. To prepare the liposomes, we have investigated two different methods : electroformation and conventional gentle-hydration method. We found that the liposomes produced by the conventional method were relatively small size, and several liposomes were enclosed inside another liposome. On the other hand, the liposomes formed by electroformation were mostly giant liposomes and did not enclose liposomes inside.
    Through these results, we have tried to make monodisperse liposomes by using microfluidic devices for emulsification, towards new technology of liposome production. It is general to change the wetting properties of the microfluidic channel for making W/O/W emulsions. Here, we have designed and fabricated axisymetric flow-focusing devices, and succeeded in making W/O/W emulsions. This method can be useful for making lipid bilayer vesicles by thinning the layer of organic solvent. Moreover, microfluidic devices are suitable for making monodisperse vesicles. We thus believe that this technology could be a route to the production of monodisperse liposomes.

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  • マイクロマシン技術の応用による噴流混合プロセスの知的制御システムの構築

    2000.4 - 2002.3

    文部科学省  科学研究費補助金(日本学術振興会・文部科学省) 

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Intellectual property rights

  • W/O/W液滴の製造方法、W/O/W液滴の製造装置およびW/O/W液滴

    牛山 諒太

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    Application no:特願2021-075103  Date applied:2021.4.27

    Announcement no:特開2022-169218  Date announced:2022.11.9

    Applicant (Organization):学校法人中央大学

  • マイクロウェルプレート、マイクロウェル装置、細胞解析方法及びマイクロウェルプレートの製造方法

    岡野 太治,城口 克之

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    Application no:特願2015-044934  Date applied:2015.3.6

    Announcement no:特開2016-163549  Date announced:2016.9.8

    Registration no:特許第6579465号  Date registered:2019.9.6 

    Applicant (Organization):学校法人中央大学、国立研究開発法人理化学研究所

  • 細胞縦断面観察用の顕微鏡観察用部材及び細胞観察方法

    津金 麻実子

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    Application no:特願2015-006085  Date applied:2015.1.15

    Announcement no:特開2016-131500  Date announced:2016.7.25

    Applicant (Organization):学校法人中央大学

  • 加速度センサ及び身体装着具

    小峯 力,檀 一平太

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    Application no:特願2014-014450  Date applied:2014.1.29

    Announcement no:特開2015-141120  Date announced:2015.8.3

    Applicant (Organization):学校法人中央大学

Committee Memberships

  • 2022.5 - Now

    化学とマイクロ・ナノシステム学会   理事  

  • 2021.4 - 2023.3

    日本機械学会   マイクロ・ナノ工学部門運営委員  

  • 2021 - 2023.3

    日本機械学会   マイクロ・ナノ工学部門表彰委員  

  • 2021.4 -  

    日本学術会議   機械工学委員会・総合工学委員会・土木工学・建築学委員会合同理論応用力学分科会 IUTAM・国際連携小委員会委員  

  • 2016.4 - 2017.3

    日本機械学会   日本機械学会学術誌校閲委員  

  • 2014.9 - 2016.8

    電気学会   マイクロ・ナノ医療デバイス調査専門委員  

  • 2014.6 - 2015.5

    電気学会   センサマイクロマシン部門論文委員  

  • 2014.6 - 2015.5

    電気学会   論文委員会(Eグループ) 委員  

  • 2013.4 - 2015.3

    日本機械学会   マイクロ・ナノ工学部門運営委員  

  • 2011.4 - 2013.3

    日本生物物理学会   若手奨励賞審査委員  

  • 2010 - 2011

    細胞を創る研究会   セッションオーガナイザー  

  • 2009.4 - 2010.3

    日本機械学会   熱工学部門 広報委員  

  • 2008 - 2010

    化学とマイクロ・ナノシステム学会   MicroTAS国際会議ポスター賞選考委員  

  • 2008 - 2010

    化学とマイクロ・ナノシステム学会   若手ポスター賞選考委員  

  • 2008 -  

    細胞を創る研究会   世話人  

  • 2007 -  

    細胞を創る研究会   発起人  

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